Restriction map for
pF1KA0800
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI Aor51HI AscI AspEI AvrII
BbeI BbrPI BcgI BciVI BfrI
BlnI BsaAI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI HpaI KasI KspI
MluI MroI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfoI SgrAI SmaI SmlI
SnaBI Sse8387I StuI SwaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 93 GGTACC G^GTACC
AccII 3892 CGCG CG^CG
AcyI 3721 GGCGTC G[GA]^CG[CT]C
AgeI 1231 ACCGGT A^CCGGT
Alw44I 1505 GTGCAC G^TGCAC
AocI 775 CCTGAGG CC^T[ACGT]AGG
ApaLI 1505 GTGCAC G^TGCAC
AseI 3823 ATTAAT AT^TAAT
AsnI 3823 ATTAAT AT^TAAT
Asp700I 390 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 93 GGTACC G^GTACC
AspI 2401 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 407 CCCGAG C^[CT]CG[GA]G
BamHI 156 GGATCC G^GATCC
BglI 406 GCCCGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 34 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 34 GCTGAGC GC^T[ACGT]AGC
BsaHI 3721 GGCGTC G[GA]^CG[CT]C
BsaI 683 GAGACC GGTCTC(1/5)
BsaWI 1231 ACCGGT [AT]^CCGG[AT]
BsoBI 407 CCCGAG C^[CT]CG[GA]G
Bsp1407I 4072 TGTACA T^GTACA
BspLU11I 18 ACATGT A^CATGT
BsrFI 1231 ACCGGT [GA]^CCGG[CT]
BsrGI 4072 TGTACA T^GTACA
BstUI 3892 CGCG CG^CG
Bsu36I 775 CCTGAGG CC^T[ACGT]AGG
CelII 34 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1231 ACCGGT [GA]^CCGG[CT]
DraI 300 TTTAAA TTT^AAA
DrdI 179 GACATCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 775 CCTGAGG CC^T[ACGT]AGG
EcoNI 4389 CCTTCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 3874 CCCGC CCCGC(4/6)
HaeII 3135 AGCGCC [GA]GCGC^[CT]
Hin1I 3721 GGCGTC G[GA]^CG[CT]C
HincII 3633 GTTGAC GT[CT]^[GA]AC
HindII 3633 GTTGAC GT[CT]^[GA]AC
HindIII 695 AAGCTT A^AGCTT
KpnI 93 GGTACC GGTAC^C
MvnI 3892 CGCG CG^CG
NdeI 272 CATATG CA^TATG
PflFI 2401 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1231 ACCGGT A^CCGGT
PshBI 3823 ATTAAT AT^TAAT
ScaI 1471 AGTACT AGT^ACT
SfiI 405 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 745 ACTAGT A^CTAGT
SspBI 4072 TGTACA T^GTACA
Tth111I 2401 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4392 TCTAGA T^CTAGA
XcmI 842 CCAAGCAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 390 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 18 ACATGT A^C[GA][CT]GT
1934 ACGTGT
ApaI 404 GGGCCC GGGCC^C
2264 GGGCCC
AviII 1176 TGCGCA TGC^GCA
1571 TGCGCA
BalI 1595 TGGCCA TGG^CCA
2165 TGGCCA
BbvCI 2791 CCTCAGC CCTCAGC(-5/-2)
1996 GCTGAGG
BclI 1466 TGATCA T^GATCA
3818 TGATCA
BglII 713 AGATCT A^GATCT
3651 AGATCT
BspHI 251 TCATGA T^CATGA
1692 TCATGA
BstBI 527 TTCGAA TT^CGAA
3845 TTCGAA
BstXI 1598 CCATTAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3798 CCATCCAAATGG
EaeI 1595 TGGCCA [CT]^GGCC[GA]
2165 TGGCCA
EcoT14I 1277 CCATGG C^C[AT][AT]GG
2940 CCATGG
EcoT22I 269 ATGCAT ATGCA^T
1294 ATGCAT
FbaI 1466 TGATCA T^GATCA
3818 TGATCA
FspI 1176 TGCGCA TGC^GCA
1571 TGCGCA
MaeII 1934 ACGT A^CGT
3151 ACGT
MluNI 1595 TGGCCA TGG^CCA
2165 TGGCCA
MscI 1595 TGGCCA TGG^CCA
2165 TGGCCA
NcoI 1277 CCATGG C^CATGG
2940 CCATGG
NsiI 269 ATGCAT ATGCA^T
1294 ATGCAT
NspV 527 TTCGAA TT^CGAA
3845 TTCGAA
PpuMI 3439 GGGTCCT [GA]G^G[AT]CC[CT]
3839 GGGACCT
RcaI 251 TCATGA T^CATGA
1692 TCATGA
SacI 37 GAGCTC GAGCT^C
1423 GAGCTC
SfuI 527 TTCGAA TT^CGAA
3845 TTCGAA
SphI 1292 GCATGC GCATG^C
1678 GCATGC
SspI 995 AATATT AAT^ATT
1527 AATATT
StyI 1277 CCATGG C^C[AT][AT]GG
2940 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MamI MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NheI NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII