Restriction map for
pF1KA0847
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AviII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspEI
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I CpoI DraIII
EagI EclXI Eco47III Eco52I FseI
FspI HaeII KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 526 GACGTC GACGT^C
AccI 365 GTAGAC GT^[AC][GT]AC
AhdI 622 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1838 CCTCAGG CC^T[ACGT]AGG
AspEI 622 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2929 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3291 CCTAGG C^CTAGG
BalI 1224 TGGCCA TGG^CCA
BamHI 3652 GGATCC G^GATCC
BlnI 3291 CCTAGG C^CTAGG
BlpI 1053 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1053 GCTTAGC GC^T[ACGT]AGC
BsgI 1436 GTGCAG GTGCAG(16/14)
BsmBI 3209 CGTCTC CGTCTC(1/5)
BsmI 392 GCATTC GAATGC(1/-1)
BspDI 260 ATCGAT AT^CGAT
BsrBI 1278 CCGCTC CCGCTC(-3/-3)
BssSI 3249 CACGAG CACGAG(-5/-1)
BstAPI 1855 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1262 GGTCACC G^GT[ACGT]ACC
BstPI 1262 GGTCACC G^GT[ACGT]ACC
Bsu36I 1838 CCTCAGG CC^T[ACGT]AGG
CelII 1053 GCTTAGC GC^T[ACGT]AGC
CfoI 1972 GCGC GCG^C
ClaI 260 ATCGAT AT^CGAT
DraI 1824 TTTAAA TTT^AAA
DrdI 1118 GACACCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 622 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1838 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1262 GGTCACC G^GT[ACGT]ACC
EcoRI 760 GAATTC G^AATTC
EcoT22I 575 ATGCAT ATGCA^T
FauI 3326 CCCGC CCCGC(4/6)
HgaI 1950 GACGC GACGC(5/10)
HhaI 1972 GCGC GCG^C
HinP1I 1972 GCGC G^CGC
HpaI 1612 GTTAAC GTT^AAC
MfeI 974 CAATTG C^AATTG
MluNI 1224 TGGCCA TGG^CCA
MscI 1224 TGGCCA TGG^CCA
MunI 974 CAATTG C^AATTG
NsiI 575 ATGCAT ATGCA^T
PaeR7I 539 CTCGAG C^TCGAG
PflFI 2929 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1862 CTGCAG CTGCA^G
SacI 1620 GAGCTC GAGCT^C
ScaI 1752 AGTACT AGT^ACT
StuI 3253 AGGCCT AGG^CCT
Tth111I 2929 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 539 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AciI 1278 CCGC CCGC(-3/-1)
3327 CCGC
AcyI 526 GACGTC G[GA]^CG[CT]C
1950 GACGCC
AlwNI 1382 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
2882 CAGTTTCTG
AvaI 326 CCCGAG C^[CT]CG[GA]G
539 CTCGAG
BanI 2499 GGTGCC G^G[CT][GA]CC
2776 GGTGCC
BanII 1620 GAGCTC G[GA]GC[CT]^C
2095 GAGCCC
BclI 1818 TGATCA T^GATCA
2280 TGATCA
BcnI 2786 CCCGG CC^[GC]GG
3365 CCCGG
BsaHI 526 GACGTC G[GA]^CG[CT]C
1950 GACGCC
BsaI 1402 GAGACC GGTCTC(1/5)
2681 GAGACC
BsoBI 326 CCCGAG C^[CT]CG[GA]G
539 CTCGAG
BspHI 2214 TCATGA T^CATGA
2651 TCATGA
BspMI 1405 ACCTGC ACCTGC(4/8)
550 GCAGGT
BsrDI 3622 CATTGC GCAATG(2/0)
1595 GCAATG
BstXI 1227 CCAGGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3166 CCAGTTTCATGG
DsaI 1221 CCATGG C^C[GA][CT]GG
2090 CCATGG
EaeI 1224 TGGCCA [CT]^GGCC[GA]
3226 CGGCCA
EcoRV 25 GATATC GAT^ATC
2631 GATATC
FbaI 1818 TGATCA T^GATCA
2280 TGATCA
Hin1I 526 GACGTC G[GA]^CG[CT]C
1950 GACGCC
MslI 642 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1516 CACTATGATG
MspA1I 667 CAGCTG C[AC]G^C[GT]G
3104 CAGCTG
NciI 2786 CCCGG CC^[GC]GG
3365 CCCGG
NcoI 1221 CCATGG C^CATGG
2090 CCATGG
PflMI 1252 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1646 CCAAAGAATGG
PvuII 667 CAGCTG CAG^CTG
3104 CAGCTG
RcaI 2214 TCATGA T^CATGA
2651 TCATGA
SfcI 1706 CTGTAG C^T[GA][CT]AG
1862 CTGCAG
Van91I 1252 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1646 CCAAAGAATGG
XbaI 774 TCTAGA T^CTAGA
3142 TCTAGA
XcmI 350 CCACTCTCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1955 CCACAAGTCTAATGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI Asp700I AspHI AvaII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI