Restriction map for
pF1KA0896
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# NO CUT ENZYMES ( 56 enzymes) #
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AccIII AclI AflII Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AviII AvrII BcgI BfrI BglI
BlnI BseAI BsiEI BsiWI BspDI
BspEI BssHII BstBI ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRV FseI FspI KspI
MluI MroI NotI NruI NspV
PacI PmeI PshBI Psp1406I PvuI
RsrII SacII SexAI SfiI SfuI
SgrAI SmaI SnaBI SwaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AgeI 3163 ACCGGT A^CCGGT
AhdI 8309 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 380 CCTTAGG CC^T[ACGT]AGG
AspEI 8309 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 7607 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 6566 GGCGCC GGCGC^C
BbrPI 1845 CACGTG CAC^GTG
BbvCI 4786 GCTGAGG CCTCAGC(-5/-2)
BsaWI 3163 ACCGGT [AT]^CCGG[AT]
BspLU11I 7653 ACATGT A^CATGT
BsrBI 1411 GAGCGG CCGCTC(-3/-3)
BstEII 835 GGTTACC G^GT[ACGT]ACC
BstPI 835 GGTTACC G^GT[ACGT]ACC
Bsu36I 380 CCTTAGG CC^T[ACGT]AGG
DraI 99 TTTAAA TTT^AAA
Eam1105I 8309 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 380 CCTTAGG CC^T[ACGT]AGG
EcoNI 428 CCTCTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 835 GGTTACC G^GT[ACGT]ACC
EcoRI 5628 GAATTC G^AATTC
EcoT22I 1856 ATGCAT ATGCA^T
HpaI 1254 GTTAAC GTT^AAC
KasI 6566 GGCGCC G^GCGCC
NaeI 464 GCCGGC GCC^GGC
NarI 6566 GGCGCC GG^CGCC
NdeI 5843 CATATG CA^TATG
NgoMIV 464 GCCGGC G^CCGGC
NsiI 1856 ATGCAT ATGCA^T
PflFI 7607 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3163 ACCGGT A^CCGGT
PmaCI 1845 CACGTG CAC^GTG
PmlI 1845 CACGTG CAC^GTG
PshAI 1898 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 449 GAGCTC GAGCT^C
SalI 2663 GTCGAC G^TCGAC
SbfI 2112 CCTGCAGG CCTGCA^GG
ScaI 4501 AGTACT AGT^ACT
SfoI 6566 GGCGCC GGC^GCC
SpeI 4212 ACTAGT A^CTAGT
SphI 2769 GCATGC GCATG^C
Sse8387I 2112 CCTGCAGG CCTGCA^GG
Tth111I 7607 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6933 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AatII 3 GACGTC GACGT^C
300 GACGTC
AflIII 1846 ACGTGT A^C[GA][CT]GT
7653 ACATGT
Asp700I 7575 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7843 GAACTCATTC
BalI 2465 TGGCCA TGG^CCA
6048 TGGCCA
BanII 449 GAGCTC G[GA]GC[CT]^C
1124 GGGCTC
BsaAI 950 TACGTG [CT]AC^GT[GA]
1845 CACGTG
BsmBI 7751 CGTCTC CGTCTC(1/5)
2976 GAGACG
BstAPI 4933 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5293 GCAGCAGCTGC
BstXI 5106 CCATGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6140 CCATCCCCTTGG
HgaI 5928 GACGC GACGC(5/10)
7750 GCGTC
MluNI 2465 TGGCCA TGG^CCA
6048 TGGCCA
MscI 2465 TGGCCA TGG^CCA
6048 TGGCCA
PflMI 12 CCATTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7543 CCAAGGCCTGG
PpuMI 1821 GGGTCCT [GA]G^G[AT]CC[CT]
3201 AGGTCCT
SapI 1942 GAAGAGC GCTCTTC(1/4)
4096 GAAGAGC
SspI 2235 AATATT AAT^ATT
2761 AATATT
Van91I 12 CCATTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7543 CCAAGGCCTGG
XmnI 7575 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7843 GAACTCATTC
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# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
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Acc65I AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI Asp718I AspHI AvaI AvaII
BamHI BanI BbsI BbvI BciVI
BclI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI Bst1107I BstF5I BstNI
BstUI BstYI BstZ17I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NcoI
NdeII NheI NlaIII NlaIV NspI
PaeR7I PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoI
XhoII