################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AccIII AclI AflII Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AviII AvrII BcgI BfrI BglI BlnI BseAI BsiEI BsiWI BspDI BspEI BssHII BstBI ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRV FseI FspI KspI MluI MroI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SacII SexAI SfiI SfuI SgrAI SmaI SnaBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AgeI 3163 ACCGGT A^CCGGT AhdI 8309 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 380 CCTTAGG CC^T[ACGT]AGG AspEI 8309 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 7607 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 6566 GGCGCC GGCGC^C BbrPI 1845 CACGTG CAC^GTG BbvCI 4786 GCTGAGG CCTCAGC(-5/-2) BsaWI 3163 ACCGGT [AT]^CCGG[AT] BspLU11I 7653 ACATGT A^CATGT BsrBI 1411 GAGCGG CCGCTC(-3/-3) BstEII 835 GGTTACC G^GT[ACGT]ACC BstPI 835 GGTTACC G^GT[ACGT]ACC Bsu36I 380 CCTTAGG CC^T[ACGT]AGG DraI 99 TTTAAA TTT^AAA Eam1105I 8309 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 380 CCTTAGG CC^T[ACGT]AGG EcoNI 428 CCTCTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 835 GGTTACC G^GT[ACGT]ACC EcoRI 5628 GAATTC G^AATTC EcoT22I 1856 ATGCAT ATGCA^T HpaI 1254 GTTAAC GTT^AAC KasI 6566 GGCGCC G^GCGCC NaeI 464 GCCGGC GCC^GGC NarI 6566 GGCGCC GG^CGCC NdeI 5843 CATATG CA^TATG NgoMIV 464 GCCGGC G^CCGGC NsiI 1856 ATGCAT ATGCA^T PflFI 7607 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3163 ACCGGT A^CCGGT PmaCI 1845 CACGTG CAC^GTG PmlI 1845 CACGTG CAC^GTG PshAI 1898 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 449 GAGCTC GAGCT^C SalI 2663 GTCGAC G^TCGAC SbfI 2112 CCTGCAGG CCTGCA^GG ScaI 4501 AGTACT AGT^ACT SfoI 6566 GGCGCC GGC^GCC SpeI 4212 ACTAGT A^CTAGT SphI 2769 GCATGC GCATG^C Sse8387I 2112 CCTGCAGG CCTGCA^GG Tth111I 7607 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 6933 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AatII 3 GACGTC GACGT^C 300 GACGTC AflIII 1846 ACGTGT A^C[GA][CT]GT 7653 ACATGT Asp700I 7575 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 7843 GAACTCATTC BalI 2465 TGGCCA TGG^CCA 6048 TGGCCA BanII 449 GAGCTC G[GA]GC[CT]^C 1124 GGGCTC BsaAI 950 TACGTG [CT]AC^GT[GA] 1845 CACGTG BsmBI 7751 CGTCTC CGTCTC(1/5) 2976 GAGACG BstAPI 4933 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5293 GCAGCAGCTGC BstXI 5106 CCATGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6140 CCATCCCCTTGG HgaI 5928 GACGC GACGC(5/10) 7750 GCGTC MluNI 2465 TGGCCA TGG^CCA 6048 TGGCCA MscI 2465 TGGCCA TGG^CCA 6048 TGGCCA PflMI 12 CCATTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 7543 CCAAGGCCTGG PpuMI 1821 GGGTCCT [GA]G^G[AT]CC[CT] 3201 AGGTCCT SapI 1942 GAAGAGC GCTCTTC(1/4) 4096 GAAGAGC SspI 2235 AATATT AAT^ATT 2761 AATATT Van91I 12 CCATTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 7543 CCAAGGCCTGG XmnI 7575 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 7843 GAACTCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BamHI BanI BbsI BbvI BciVI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI Bst1107I BstF5I BstNI BstUI BstYI BstZ17I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PaeR7I PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII