Restriction map for
pF1KA1159
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BamHI BbeI
BbrPI BbsI BciVI BclI BfrI
BglI BpuAI BsaAI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MfeI MluI MroI MseI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I TspRI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 661 GTCTAC GT^[AC][GT]AC
AspHI 615 GAGCTC G[AT]GC[AT]^C
AvrII 195 CCTAGG C^CTAGG
BanI 135 GGTGCC G^G[CT][GA]CC
BanII 615 GAGCTC G[GA]GC[CT]^C
BbvCI 275 CCTCAGC CCTCAGC(-5/-2)
BcgI 3 GCAACTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 196 CTAG C^TAG
BlnI 195 CCTAGG C^CTAGG
BlpI 617 GCTCAGC GC^T[ACGT]AGC
BpmI 627 CTGGAG CTGGAG(16/14)
Bpu1102I 617 GCTCAGC GC^T[ACGT]AGC
BsaBI 486 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 602 GAGACC GGTCTC(1/5)
BsgI 34 GTGCAG GTGCAG(16/14)
BsiHKAI 615 GAGCTC G[AT]GC[AT]^C
BsmAI 602 GAGAC GTCTC(1/5)
BsmI 230 GCATTC GAATGC(1/-1)
BspMI 344 ACCTGC ACCTGC(4/8)
BsrDI 377 GCAATG GCAATG(2/0)
BsrI 452 CCAGT ACTGG(1/-1)
CelII 617 GCTCAGC GC^T[ACGT]AGC
EcoNI 30 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 392 GCGTC GACGC(5/10)
HinfI 9 GACTC G^A[ACGT]TC
MaeI 196 CTAG C^TAG
MaeIII 352 GTCAC ^GT[ACGT]AC
MamI 486 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 373 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 9 GACTC GAGTC(4/5)
SacI 615 GAGCTC GAGCT^C
SmaI 131 CCCGGG CCC^GGG
StuI 503 AGGCCT AGG^CCT
TaqI 494 TCGA T^CGA
Tsp45I 352 GTCAC ^GT[GC]AC
Tsp509I 184 AATT ^AATT
TthHB8I 494 TCGA T^CGA
XmaCI 131 CCCGGG C^CCGGG
XmaI 131 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaI 131 CCCGGG C^[CT]CG[GA]G
599 CCCGAG
BalI 63 TGGCCA TGG^CCA
179 TGGCCA
BglII 485 AGATCT A^GATCT
590 AGATCT
BsmFI 382 GGGAC GGGAC(10/14)
168 GTCCC
BsoBI 131 CCCGGG C^[CT]CG[GA]G
599 CCCGAG
BssSI 112 CACGAG CACGAG(-5/-1)
442 CTCGTG
BstF5I 69 GGATG GGATG(2/0)
527 GGATG
BstXI 66 CCAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
372 CCATGGCAATGG
BstYI 485 AGATCT [GA]^GATC[CT]
590 AGATCT
Cac8I 119 GCCAGC GC[ACGT]^[ACGT]GC
204 GCTGGC
DpnII 486 GATC ^GATC
591 GATC
DraII 92 AGGACCC [GA]G^G[ACGT]CC[CT]
382 GGGACCT
DsaI 176 CCATGG C^C[GA][CT]GG
372 CCATGG
EaeI 63 TGGCCA [CT]^GGCC[GA]
179 TGGCCA
EcoO109I 92 AGGACCC [GA]G^G[ACGT]CC[CT]
382 GGGACCT
FokI 69 GGATG GGATG(9/13)
527 GGATG
HphI 285 GGTGA GGTGA(8/7)
160 TCACC
MboI 486 GATC ^GATC
591 GATC
MflI 485 AGATCT [GA]^GATC[CT]
590 AGATCT
MluNI 63 TGGCCA TGG^CCA
179 TGGCCA
MscI 63 TGGCCA TGG^CCA
179 TGGCCA
NcoI 176 CCATGG C^CATGG
372 CCATGG
NdeII 486 GATC ^GATC
591 GATC
NlaIII 177 CATG CATG^
373 CATG
PpuMI 92 AGGACCC [GA]G^G[AT]CC[CT]
382 GGGACCT
Sau3AI 486 GATC ^GATC
591 GATC
SfcI 461 CTGTAG C^T[GA][CT]AG
675 CTACAG
XhoII 485 AGATCT [GA]^GATC[CT]
590 AGATCT
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI Cfr13I DdeI EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HpaII ItaI MboII MnlI MspI
MvaI MwoI NciI NlaIV Sau96I
ScrFI StyI TseI