################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BbeI BbrPI BbsI BciVI BclI BfrI BglI BpuAI BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MfeI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I TspRI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 661 GTCTAC GT^[AC][GT]AC AspHI 615 GAGCTC G[AT]GC[AT]^C AvrII 195 CCTAGG C^CTAGG BanI 135 GGTGCC G^G[CT][GA]CC BanII 615 GAGCTC G[GA]GC[CT]^C BbvCI 275 CCTCAGC CCTCAGC(-5/-2) BcgI 3 GCAACTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 196 CTAG C^TAG BlnI 195 CCTAGG C^CTAGG BlpI 617 GCTCAGC GC^T[ACGT]AGC BpmI 627 CTGGAG CTGGAG(16/14) Bpu1102I 617 GCTCAGC GC^T[ACGT]AGC BsaBI 486 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 602 GAGACC GGTCTC(1/5) BsgI 34 GTGCAG GTGCAG(16/14) BsiHKAI 615 GAGCTC G[AT]GC[AT]^C BsmAI 602 GAGAC GTCTC(1/5) BsmI 230 GCATTC GAATGC(1/-1) BspMI 344 ACCTGC ACCTGC(4/8) BsrDI 377 GCAATG GCAATG(2/0) BsrI 452 CCAGT ACTGG(1/-1) CelII 617 GCTCAGC GC^T[ACGT]AGC EcoNI 30 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 392 GCGTC GACGC(5/10) HinfI 9 GACTC G^A[ACGT]TC MaeI 196 CTAG C^TAG MaeIII 352 GTCAC ^GT[ACGT]AC MamI 486 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 373 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PleI 9 GACTC GAGTC(4/5) SacI 615 GAGCTC GAGCT^C SmaI 131 CCCGGG CCC^GGG StuI 503 AGGCCT AGG^CCT TaqI 494 TCGA T^CGA Tsp45I 352 GTCAC ^GT[GC]AC Tsp509I 184 AATT ^AATT TthHB8I 494 TCGA T^CGA XmaCI 131 CCCGGG C^CCGGG XmaI 131 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AvaI 131 CCCGGG C^[CT]CG[GA]G 599 CCCGAG BalI 63 TGGCCA TGG^CCA 179 TGGCCA BglII 485 AGATCT A^GATCT 590 AGATCT BsmFI 382 GGGAC GGGAC(10/14) 168 GTCCC BsoBI 131 CCCGGG C^[CT]CG[GA]G 599 CCCGAG BssSI 112 CACGAG CACGAG(-5/-1) 442 CTCGTG BstF5I 69 GGATG GGATG(2/0) 527 GGATG BstXI 66 CCAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 372 CCATGGCAATGG BstYI 485 AGATCT [GA]^GATC[CT] 590 AGATCT Cac8I 119 GCCAGC GC[ACGT]^[ACGT]GC 204 GCTGGC DpnII 486 GATC ^GATC 591 GATC DraII 92 AGGACCC [GA]G^G[ACGT]CC[CT] 382 GGGACCT DsaI 176 CCATGG C^C[GA][CT]GG 372 CCATGG EaeI 63 TGGCCA [CT]^GGCC[GA] 179 TGGCCA EcoO109I 92 AGGACCC [GA]G^G[ACGT]CC[CT] 382 GGGACCT FokI 69 GGATG GGATG(9/13) 527 GGATG HphI 285 GGTGA GGTGA(8/7) 160 TCACC MboI 486 GATC ^GATC 591 GATC MflI 485 AGATCT [GA]^GATC[CT] 590 AGATCT MluNI 63 TGGCCA TGG^CCA 179 TGGCCA MscI 63 TGGCCA TGG^CCA 179 TGGCCA NcoI 176 CCATGG C^CATGG 372 CCATGG NdeII 486 GATC ^GATC 591 GATC NlaIII 177 CATG CATG^ 373 CATG PpuMI 92 AGGACCC [GA]G^G[AT]CC[CT] 382 GGGACCT Sau3AI 486 GATC ^GATC 591 GATC SfcI 461 CTGTAG C^T[GA][CT]AG 675 CTACAG XhoII 485 AGATCT [GA]^GATC[CT] 590 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DdeI EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI StyI TseI