Restriction map for
pF1KA1209
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BsaAI BsaHI BseAI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DraIII EagI EclXI
Eco52I EcoO65I EcoRI EcoT22I FseI
FspI Hin1I HindIII KasI KpnI
KspI MluI MroI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 1244 ACACGT A^C[GA][CT]GT
AhdI 3487 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3691 AGCGCT AGC^GCT
ApaI 3259 GGGCCC GGGCC^C
Asp700I 4297 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3487 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2149 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 2820 CCGGG CC^[GC]GG
BsaBI 1846 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1079 TCCGGT [AT]^CCGG[AT]
BsiEI 479 CGATCG CG[GA][CT]^CG
Bsp1407I 561 TGTACA T^GTACA
BsrDI 4136 CATTGC GCAATG(2/0)
BsrGI 561 TGTACA T^GTACA
CpoI 1076 CGGTCCG CG^G[AT]CCG
DrdI 196 GACCGACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3487 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3691 AGCGCT AGC^GCT
HpaI 4177 GTTAAC GTT^AAC
MamI 1846 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3541 CAATTG C^AATTG
MunI 3541 CAATTG C^AATTG
NaeI 3189 GCCGGC GCC^GGC
NciI 2820 CCGGG CC^[GC]GG
NdeI 1942 CATATG CA^TATG
NgoMIV 3189 GCCGGC G^CCGGC
NspI 2026 ACATGC [GA]CATG^[CT]
PflFI 2149 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 479 CGATCG CGAT^CG
RsrII 1076 CGGTCCG CG^G[AT]CCG
ScaI 3529 AGTACT AGT^ACT
SpeI 2047 ACTAGT A^CTAGT
SspBI 561 TGTACA T^GTACA
SspI 4276 AATATT AAT^ATT
Tth111I 2149 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1651 TCTAGA T^CTAGA
XmnI 4297 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BclI 1733 TGATCA T^GATCA
3740 TGATCA
BglI 290 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2781 GCCAGACAGGC
BglII 1884 AGATCT A^GATCT
3891 AGATCT
BsaI 1966 GAGACC GGTCTC(1/5)
2738 GAGACC
BsmBI 1691 CGTCTC CGTCTC(1/5)
2743 CGTCTC
BsmI 3432 GCATTC GAATGC(1/-1)
4140 GCATTC
BssSI 495 CACGAG CACGAG(-5/-1)
1066 CACGAG
BstXI 329 CCATAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3408 CCATGGTAGTGG
DraI 570 TTTAAA TTT^AAA
3156 TTTAAA
EcoRV 670 GATATC GAT^ATC
3500 GATATC
FbaI 1733 TGATCA T^GATCA
3740 TGATCA
HaeII 2831 GGCGCT [GA]GCGC^[CT]
3691 AGCGCT
HincII 2130 GTTGAC GT[CT]^[GA]AC
4177 GTTAAC
HindII 2130 GTTGAC GT[CT]^[GA]AC
4177 GTTAAC
NcoI 249 CCATGG C^CATGG
3408 CCATGG
PpuMI 901 GGGTCCT [GA]G^G[AT]CC[CT]
3715 AGGTCCC
SacI 181 GAGCTC GAGCT^C
1757 GAGCTC
SapI 2886 GAAGAGC GCTCTTC(1/4)
1197 GCTCTTC
TaqI 1208 TCGA T^CGA
1746 TCGA
TthHB8I 1208 TCGA T^CGA
1746 TCGA
XcmI 3399 CCAGGTCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3405 CCACCATGGTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII