Restriction map for
pF1KA1286
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AspEI AvrII
BalI BbrPI BbvCI BcgI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI DrdI Eam1105I Eco47III
EcoO65I FseI HpaI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 2749 GGTACC G^GTACC
AcsI 1882 GAATTC [GA]^AATT[CT]
AflIII 1051 ACATGT A^C[GA][CT]GT
Alw44I 1055 GTGCAC G^TGCAC
AocI 748 CCTGAGG CC^T[ACGT]AGG
ApaLI 1055 GTGCAC G^TGCAC
ApoI 1882 GAATTC [GA]^AATT[CT]
AseI 2584 ATTAAT AT^TAAT
AsnI 2584 ATTAAT AT^TAAT
Asp700I 3393 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2749 GGTACC G^GTACC
AviII 1130 TGCGCA TGC^GCA
BbeI 829 GGCGCC GGCGC^C
BciVI 2389 GTATCC GTATCC(6/5)
BclI 3278 TGATCA T^GATCA
BglII 1457 AGATCT A^GATCT
BlpI 1597 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1597 GCTGAGC GC^T[ACGT]AGC
BsiEI 2744 CGGCCG CG[GA][CT]^CG
Bsp1407I 714 TGTACA T^GTACA
BspHI 1076 TCATGA T^CATGA
BspLU11I 1051 ACATGT A^CATGT
BsrBI 1678 GAGCGG CCGCTC(-3/-3)
BsrDI 677 GCAATG GCAATG(2/0)
BsrGI 714 TGTACA T^GTACA
Bsu36I 748 CCTGAGG CC^T[ACGT]AGG
CelII 1597 GCTGAGC GC^T[ACGT]AGC
EagI 2744 CGGCCG C^GGCCG
EclXI 2744 CGGCCG C^GGCCG
Eco52I 2744 CGGCCG C^GGCCG
Eco81I 748 CCTGAGG CC^T[ACGT]AGG
EcoRI 1882 GAATTC G^AATTC
EcoRV 208 GATATC GAT^ATC
EcoT22I 317 ATGCAT ATGCA^T
FbaI 3278 TGATCA T^GATCA
FspI 1130 TGCGCA TGC^GCA
HincII 1852 GTCAAC GT[CT]^[GA]AC
HindII 1852 GTCAAC GT[CT]^[GA]AC
HindIII 3074 AAGCTT A^AGCTT
KasI 829 GGCGCC G^GCGCC
KpnI 2749 GGTACC GGTAC^C
KspI 3210 CCGCGG CCGC^GG
NaeI 41 GCCGGC GCC^GGC
NarI 829 GGCGCC GG^CGCC
NdeI 98 CATATG CA^TATG
NgoMIV 41 GCCGGC G^CCGGC
NsiI 317 ATGCAT ATGCA^T
PaeR7I 3128 CTCGAG C^TCGAG
PshBI 2584 ATTAAT AT^TAAT
RcaI 1076 TCATGA T^CATGA
SacI 2641 GAGCTC GAGCT^C
SacII 3210 CCGCGG CCGC^GG
SfoI 829 GGCGCC GGC^GCC
SphI 315 GCATGC GCATG^C
SspBI 714 TGTACA T^GTACA
StuI 2444 AGGCCT AGG^CCT
XhoI 3128 CTCGAG C^TCGAG
XmnI 3393 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 127 GTAGAC GT^[AC][GT]AC
562 GTAGAC
AspI 2833 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3451 GACAAAGTC
BamHI 884 GGATCC G^GATCC
1824 GGATCC
BspDI 625 ATCGAT AT^CGAT
3283 ATCGAT
ClaI 625 ATCGAT AT^CGAT
3283 ATCGAT
DraIII 1163 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2756 CACTTAGTG
MaeII 2177 ACGT A^CGT
3350 ACGT
NcoI 1757 CCATGG C^CATGG
2859 CCATGG
NspI 315 GCATGC [GA]CATG^[CT]
1051 ACATGT
PflFI 2833 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3451 GACAAAGTC
PflMI 2755 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2859 CCATGGTGTGG
SexAI 1164 ACCAGGT A^CC[AT]GGT
2258 ACCTGGT
SmaI 83 CCCGGG CCC^GGG
3299 CCCGGG
Tth111I 2833 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3451 GACAAAGTC
Van91I 2755 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2859 CCATGGTGTGG
XcmI 350 CCAGCTTCCGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
720 CCAGGAGTGCTATGG
XmaCI 83 CCCGGG C^CCGGG
3299 CCCGGG
XmaI 83 CCCGGG C^CCGGG
3299 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII