Restriction map for
pF1KA1287
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI ApaI AscI AseI AsnI
Asp700I Asp718I AvaI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglII BlnI BsaAI
BsiWI BsoBI BspDI BspHI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI DraI
DrdI EagI EclXI Eco52I EcoO65I
FauI FseI FspI KasI KpnI
KspI MluI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 1411 GGCGTC G[GA]^CG[CT]C
AhdI 1245 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2201 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2580 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2917 AGCGCT AGC^GCT
AspEI 1245 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1489 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 644 TGGCCA TGG^CCA
BanI 619 GGTGCC G^G[CT][GA]CC
BclI 2589 TGATCA T^GATCA
BglI 2472 GCCTAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1411 GGCGTC G[GA]^CG[CT]C
BsgI 2807 GTGCAG GTGCAG(16/14)
BsiEI 3083 CGGTCG CG[GA][CT]^CG
BsrBI 2795 CCGCTC CCGCTC(-3/-3)
BstXI 3469 CCAAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2580 CCTAAGG CC^T[ACGT]AGG
Eam1105I 1245 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2917 AGCGCT AGC^GCT
Eco81I 2580 CCTAAGG CC^T[ACGT]AGG
EcoNI 1538 CCTTGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2648 GATATC GAT^ATC
FbaI 2589 TGATCA T^GATCA
HaeII 2917 AGCGCT [GA]GCGC^[CT]
Hin1I 1411 GGCGTC G[GA]^CG[CT]C
MfeI 2726 CAATTG C^AATTG
MluNI 644 TGGCCA TGG^CCA
MscI 644 TGGCCA TGG^CCA
MunI 2726 CAATTG C^AATTG
NdeI 2363 CATATG CA^TATG
PflFI 1489 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 823 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1610 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 559 GAGCTC GAGCT^C
ScaI 1402 AGTACT AGT^ACT
SspI 1876 AATATT AAT^ATT
Tth111I 1489 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 823 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3114 TCTAGA T^CTAGA
XcmI 1884 CCAAGGAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccI 2541 GTATAC GT^[AC][GT]AC
3194 GTATAC
AccIII 938 TCCGGA T^CCGGA
2754 TCCGGA
Alw44I 164 GTGCAC G^TGCAC
1157 GTGCAC
ApaLI 164 GTGCAC G^TGCAC
1157 GTGCAC
AvaII 789 GGTCC G^G[AT]CC
1611 GGTCC
BamHI 3514 GGATCC G^GATCC
3537 GGATCC
BpmI 227 CTGGAG CTGGAG(16/14)
1033 CTGGAG
BsaBI 1185 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2058 GATTCCTATC
BsaWI 938 TCCGGA [AT]^CCGG[AT]
2754 TCCGGA
BseAI 938 TCCGGA T^CCGGA
2754 TCCGGA
BseRI 329 GAGGAG GAGGAG(10/8)
2851 GAGGAG
BsmBI 1413 CGTCTC CGTCTC(1/5)
2134 CGTCTC
BspEI 938 TCCGGA T^CCGGA
2754 TCCGGA
BspLU11I 606 ACATGT A^CATGT
2636 ACATGT
Bst1107I 2541 GTATAC GTA^TAC
3194 GTATAC
BstAPI 157 GCACTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1227 GCAATTACTGC
BstZ17I 2541 GTATAC GTA^TAC
3194 GTATAC
CfoI 732 GCGC GCG^C
2918 GCGC
DsaI 266 CCGTGG C^C[GA][CT]GG
2416 CCACGG
EaeI 644 TGGCCA [CT]^GGCC[GA]
2792 TGGCCG
EcoRI 2403 GAATTC G^AATTC
2423 GAATTC
EcoT14I 828 CCTTGG C^C[AT][AT]GG
1884 CCAAGG
EcoT22I 2783 ATGCAT ATGCA^T
3203 ATGCAT
HapII 939 CCGG C^CGG
2755 CCGG
HhaI 732 GCGC GCG^C
2918 GCGC
HinP1I 732 GCGC G^CGC
2918 GCGC
HpaI 2376 GTTAAC GTT^AAC
3261 GTTAAC
HpaII 939 CCGG C^CGG
2755 CCGG
MamI 1185 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2058 GATTCCTATC
MroI 938 TCCGGA T^CCGGA
2754 TCCGGA
MspI 939 CCGG C^CGG
2755 CCGG
NsiI 2783 ATGCAT ATGCA^T
3203 ATGCAT
SapI 1135 GAAGAGC GCTCTTC(1/4)
1981 GCTCTTC
StuI 825 AGGCCT AGG^CCT
2707 AGGCCT
StyI 828 CCTTGG C^C[AT][AT]GG
1884 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI BanII BbsI
BbvI BfaI BlpI BmyI Bpu1102I
BpuAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraII
DraIII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HgaI HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII