################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BglI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspMI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FseI FspI HgaI Hin1I KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NgoMIV NheI NotI NruI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AclI 1887 AACGTT AA^CGTT BciVI 2187 GGATAC GTATCC(6/5) BcnI 5799 CCGGG CC^[GC]GG BsaAI 5047 TACGTA [CT]AC^GT[GA] BseRI 59 CTCCTC GAGGAG(10/8) BsgI 663 CTGCAC GTGCAG(16/14) BsmFI 5476 GGGAC GGGAC(10/14) BsmI 3516 GAATGC GAATGC(1/-1) BsrFI 4188 GCCGGT [GA]^CCGG[CT] BstXI 5081 CCAAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 4188 GCCGGT [GA]^CCGG[CT] DraIII 2909 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1868 CCATGG C^C[GA][CT]GG HpaI 3982 GTTAAC GTT^AAC MfeI 2300 CAATTG C^AATTG MunI 2300 CAATTG C^AATTG NciI 5799 CCGGG CC^[GC]GG NcoI 1868 CCATGG C^CATGG NdeI 1811 CATATG CA^TATG PacI 3235 TTAATTAA TTAAT^TAA PpuMI 1655 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1887 AACGTT AA^CGTT SacI 4845 GAGCTC GAGCT^C SnaBI 5047 TACGTA TAC^GTA StuI 5347 AGGCCT AGG^CCT SwaI 5150 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1003 GTAGAC GT^[AC][GT]AC 4233 GTCTAC Aor51HI 127 AGCGCT AGC^GCT 3752 AGCGCT AseI 2107 ATTAAT AT^TAAT 3183 ATTAAT AsnI 2107 ATTAAT AT^TAAT 3183 ATTAAT Asp700I 5029 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5507 GAAACATTTC BanII 2014 GAGCCC G[GA]GC[CT]^C 4845 GAGCTC BspHI 2505 TCATGA T^CATGA 4833 TCATGA BsrDI 4184 CATTGC GCAATG(2/0) 1615 GCAATG BstAPI 4283 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5710 GCAGCACTTGC BstBI 437 TTCGAA TT^CGAA 906 TTCGAA CfoI 128 GCGC GCG^C 3753 GCGC DraII 1655 AGGACCT [GA]G^G[ACGT]CC[CT] 5645 AGGCCCC Eco47III 127 AGCGCT AGC^GCT 3752 AGCGCT EcoO109I 1655 AGGACCT [GA]G^G[ACGT]CC[CT] 5645 AGGCCCC EcoRI 2778 GAATTC G^AATTC 5095 GAATTC HaeII 127 AGCGCT [GA]GCGC^[CT] 3752 AGCGCT HapII 4189 CCGG C^CGG 5799 CCGG HhaI 128 GCGC GCG^C 3753 GCGC HinP1I 128 GCGC G^CGC 3753 GCGC HindIII 3433 AAGCTT A^AGCTT 3465 AAGCTT HpaII 4189 CCGG C^CGG 5799 CCGG MspI 4189 CCGG C^CGG 5799 CCGG NspV 437 TTCGAA TT^CGAA 906 TTCGAA PflMI 2469 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2908 CCACGACGTGG PshBI 2107 ATTAAT AT^TAAT 3183 ATTAAT RcaI 2505 TCATGA T^CATGA 4833 TCATGA SfuI 437 TTCGAA TT^CGAA 906 TTCGAA SphI 3837 GCATGC GCATG^C 4587 GCATGC Van91I 2469 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2908 CCACGACGTGG XmnI 5029 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5507 GAAACATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AspHI AvaII BbsI BbvI BclI BfaI BfrI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I Bsp1407I BspLU11I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII