################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AccIII AclI AflII AscI AseI AsnI AvrII BclI BfrI BlnI BsaI BseAI BspEI BspHI BspLU11I Bst1107I BstXI BstZ17I CpoI DraI DrdI EcoRV EcoT22I FbaI HincII HindII HpaI MfeI MluI MroI MunI NdeI NheI NruI NsiI PacI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SnaBI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AflIII 1655 ACGTGT A^C[GA][CT]GT AgeI 1812 ACCGGT A^CCGGT BbrPI 2332 CACGTG CAC^GTG BciVI 3519 GGATAC GTATCC(6/5) BsaBI 4728 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 2766 CGTACG C^GTACG BspDI 5322 ATCGAT AT^CGAT BssHII 5580 GCGCGC G^CGCGC BstBI 3289 TTCGAA TT^CGAA ClaI 5322 ATCGAT AT^CGAT FseI 1576 GGCCGGCC GGCCGG^CC HindIII 5434 AAGCTT A^AGCTT MamI 4728 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1379 TTAA T^TAA NotI 4867 GCGGCCGC GC^GGCCGC NspV 3289 TTCGAA TT^CGAA PaeR7I 4900 CTCGAG C^TCGAG PinAI 1812 ACCGGT A^CCGGT PmaCI 2332 CACGTG CAC^GTG PmlI 2332 CACGTG CAC^GTG SfuI 3289 TTCGAA TT^CGAA SgrAI 1811 CACCGGTG C[GA]^CCGG[CT]G Tru9I 1379 TTAA T^TAA XhoI 4900 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 3845 GACGTC GACGT^C 5389 GACGTC Acc65I 3622 GGTACC G^GTACC 3979 GGTACC AccI 1819 GTCTAC GT^[AC][GT]AC 5116 GTCTAC Aor51HI 1219 AGCGCT AGC^GCT 3809 AGCGCT Asp718I 3622 GGTACC G^GTACC 3979 GGTACC AviII 1700 TGCGCA TGC^GCA 3119 TGCGCA BamHI 293 GGATCC G^GATCC 1973 GGATCC BbvCI 3547 GCTGAGG CCTCAGC(-5/-2) 4606 GCTGAGG BglII 872 AGATCT A^GATCT 5342 AGATCT BsmBI 479 GAGACG CGTCTC(1/5) 5686 GAGACG BsmI 271 GAATGC GAATGC(1/-1) 3382 GCATTC BsrBI 966 CCGCTC CCGCTC(-3/-3) 622 GAGCGG DraIII 2329 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 3157 CACTACGTG Eco47III 1219 AGCGCT AGC^GCT 3809 AGCGCT EcoRI 229 GAATTC G^AATTC 3127 GAATTC FspI 1700 TGCGCA TGC^GCA 3119 TGCGCA KpnI 3622 GGTACC GGTAC^C 3979 GGTACC NcoI 3644 CCATGG C^CATGG 5084 CCATGG SexAI 221 ACCTGGT A^CC[AT]GGT 4792 ACCTGGT SphI 1172 GCATGC GCATG^C 2087 GCATGC TfiI 1593 GATTC G^A[AT]TC 4038 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 160 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AhdI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp700I AspEI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspBI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI