################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AseI AsnI Asp718I AspEI AvrII BamHI BciVI BfrI BglII BlnI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoRV EcoT22I FseI KpnI MfeI MluI MunI NheI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SbfI ScaI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 3370 GACGTC GACGT^C AccI 84 GTCGAC GT^[AC][GT]AC AccIII 2789 TCCGGA T^CCGGA AcsI 471 GAATTC [GA]^AATT[CT] AfaI 3840 GTAC GT^AC AflIII 4040 ACACGT A^C[GA][CT]GT ApoI 471 GAATTC [GA]^AATT[CT] AscI 4541 GGCGCGCC GG^CGCGCC Asp700I 3317 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 3236 TGCGCA TGC^GCA BbrPI 2755 CACGTG CAC^GTG BclI 252 TGATCA T^GATCA BlpI 2940 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2940 GCTCAGC GC^T[ACGT]AGC BsaBI 253 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2789 TCCGGA [AT]^CCGG[AT] BseAI 2789 TCCGGA T^CCGGA BspEI 2789 TCCGGA T^CCGGA BspHI 255 TCATGA T^CATGA BsrFI 4453 GCCGGC [GA]^CCGG[CT] BssHII 4542 GCGCGC G^CGCGC BstBI 492 TTCGAA TT^CGAA BstEII 2524 GGTGACC G^GT[ACGT]ACC BstPI 2524 GGTGACC G^GT[ACGT]ACC BstXI 4109 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2940 GCTCAGC GC^T[ACGT]AGC Cfr10I 4453 GCCGGC [GA]^CCGG[CT] DraIII 3696 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 2524 GGTGACC G^GT[ACGT]ACC EcoRI 471 GAATTC G^AATTC FbaI 252 TGATCA T^GATCA FspI 3236 TGCGCA TGC^GCA HindIII 2926 AAGCTT A^AGCTT HpaI 4524 GTTAAC GTT^AAC KspI 3758 CCGCGG CCGC^GG MamI 253 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2789 TCCGGA T^CCGGA MseI 4525 TTAA T^TAA MslI 2715 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 4453 GCCGGC GCC^GGC NdeI 110 CATATG CA^TATG NgoMIV 4453 GCCGGC G^CCGGC NotI 4078 GCGGCCGC GC^GGCCGC NspV 492 TTCGAA TT^CGAA PflMI 574 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2755 CACGTG CAC^GTG PmlI 2755 CACGTG CAC^GTG PshAI 2403 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 255 TCATGA T^CATGA RsaI 3840 GTAC GT^AC SacII 3758 CCGCGG CCGC^GG SalI 84 GTCGAC G^TCGAC SexAI 4025 ACCAGGT A^CC[AT]GGT SfuI 492 TTCGAA TT^CGAA SmlI 3415 CTCAAG C^T[CT][GA]AG Tru9I 4525 TTAA T^TAA Van91I 574 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3317 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspI 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3103 GACGAGGTC AvaI 2699 CCCGGG C^[CT]CG[GA]G 3959 CCCGGG BbeI 3931 GGCGCC GGCGC^C 4287 GGCGCC BcgI 4194 CGATGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3402 GCAGCAGACTCG BsaAI 2755 CACGTG [CT]AC^GT[GA] 4240 CACGTA BsaI 3878 GAGACC GGTCTC(1/5) 2409 GGTCTC BsmBI 3805 GAGACG CGTCTC(1/5) 3859 GAGACG BsoBI 2699 CCCGGG C^[CT]CG[GA]G 3959 CCCGGG BssSI 2886 CACGAG CACGAG(-5/-1) 4551 CACGAG HincII 84 GTCGAC GT[CT]^[GA]AC 4524 GTTAAC HindII 84 GTCGAC GT[CT]^[GA]AC 4524 GTTAAC KasI 3931 GGCGCC G^GCGCC 4287 GGCGCC NarI 3931 GGCGCC GG^CGCC 4287 GGCGCC NcoI 3530 CCATGG C^CATGG 3638 CCATGG PflFI 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3103 GACGAGGTC SapI 3888 GAAGAGC GCTCTTC(1/4) 4233 GAAGAGC SfoI 3931 GGCGCC GGC^GCC 4287 GGCGCC SmaI 2699 CCCGGG CCC^GGG 3959 CCCGGG SphI 2732 GCATGC GCATG^C 3869 GCATGC StuI 3557 AGGCCT AGG^CCT 4484 AGGCCT Tth111I 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3103 GACGAGGTC XmaCI 2699 CCCGGG C^CCGGG 3959 CCCGGG XmaI 2699 CCCGGG C^CCGGG 3959 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI ApaI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII