Restriction map for
pF1KA1398
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AseI
AsnI Asp718I AspEI AvrII BamHI
BciVI BfrI BglII BlnI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoRV EcoT22I FseI
KpnI MfeI MluI MunI NheI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SbfI ScaI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 3370 GACGTC GACGT^C
AccI 84 GTCGAC GT^[AC][GT]AC
AccIII 2789 TCCGGA T^CCGGA
AcsI 471 GAATTC [GA]^AATT[CT]
AfaI 3840 GTAC GT^AC
AflIII 4040 ACACGT A^C[GA][CT]GT
ApoI 471 GAATTC [GA]^AATT[CT]
AscI 4541 GGCGCGCC GG^CGCGCC
Asp700I 3317 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3236 TGCGCA TGC^GCA
BbrPI 2755 CACGTG CAC^GTG
BclI 252 TGATCA T^GATCA
BlpI 2940 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2940 GCTCAGC GC^T[ACGT]AGC
BsaBI 253 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2789 TCCGGA [AT]^CCGG[AT]
BseAI 2789 TCCGGA T^CCGGA
BspEI 2789 TCCGGA T^CCGGA
BspHI 255 TCATGA T^CATGA
BsrFI 4453 GCCGGC [GA]^CCGG[CT]
BssHII 4542 GCGCGC G^CGCGC
BstBI 492 TTCGAA TT^CGAA
BstEII 2524 GGTGACC G^GT[ACGT]ACC
BstPI 2524 GGTGACC G^GT[ACGT]ACC
BstXI 4109 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2940 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4453 GCCGGC [GA]^CCGG[CT]
DraIII 3696 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2524 GGTGACC G^GT[ACGT]ACC
EcoRI 471 GAATTC G^AATTC
FbaI 252 TGATCA T^GATCA
FspI 3236 TGCGCA TGC^GCA
HindIII 2926 AAGCTT A^AGCTT
HpaI 4524 GTTAAC GTT^AAC
KspI 3758 CCGCGG CCGC^GG
MamI 253 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2789 TCCGGA T^CCGGA
MseI 4525 TTAA T^TAA
MslI 2715 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 4453 GCCGGC GCC^GGC
NdeI 110 CATATG CA^TATG
NgoMIV 4453 GCCGGC G^CCGGC
NotI 4078 GCGGCCGC GC^GGCCGC
NspV 492 TTCGAA TT^CGAA
PflMI 574 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2755 CACGTG CAC^GTG
PmlI 2755 CACGTG CAC^GTG
PshAI 2403 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 255 TCATGA T^CATGA
RsaI 3840 GTAC GT^AC
SacII 3758 CCGCGG CCGC^GG
SalI 84 GTCGAC G^TCGAC
SexAI 4025 ACCAGGT A^CC[AT]GGT
SfuI 492 TTCGAA TT^CGAA
SmlI 3415 CTCAAG C^T[CT][GA]AG
Tru9I 4525 TTAA T^TAA
Van91I 574 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3317 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspI 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3103 GACGAGGTC
AvaI 2699 CCCGGG C^[CT]CG[GA]G
3959 CCCGGG
BbeI 3931 GGCGCC GGCGC^C
4287 GGCGCC
BcgI 4194 CGATGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3402 GCAGCAGACTCG
BsaAI 2755 CACGTG [CT]AC^GT[GA]
4240 CACGTA
BsaI 3878 GAGACC GGTCTC(1/5)
2409 GGTCTC
BsmBI 3805 GAGACG CGTCTC(1/5)
3859 GAGACG
BsoBI 2699 CCCGGG C^[CT]CG[GA]G
3959 CCCGGG
BssSI 2886 CACGAG CACGAG(-5/-1)
4551 CACGAG
HincII 84 GTCGAC GT[CT]^[GA]AC
4524 GTTAAC
HindII 84 GTCGAC GT[CT]^[GA]AC
4524 GTTAAC
KasI 3931 GGCGCC G^GCGCC
4287 GGCGCC
NarI 3931 GGCGCC GG^CGCC
4287 GGCGCC
NcoI 3530 CCATGG C^CATGG
3638 CCATGG
PflFI 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3103 GACGAGGTC
SapI 3888 GAAGAGC GCTCTTC(1/4)
4233 GAAGAGC
SfoI 3931 GGCGCC GGC^GCC
4287 GGCGCC
SmaI 2699 CCCGGG CCC^GGG
3959 CCCGGG
SphI 2732 GCATGC GCATG^C
3869 GCATGC
StuI 3557 AGGCCT AGG^CCT
4484 AGGCCT
Tth111I 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3103 GACGAGGTC
XmaCI 2699 CCCGGG C^CCGGG
3959 CCCGGG
XmaI 2699 CCCGGG C^CCGGG
3959 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII