Restriction map for
pF1KA1419
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# NO CUT ENZYMES ( 47 enzymes) #
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Acc65I AclI AflII AgeI Asp718I
BbeI BcgI BfrI BsaBI BsiEI
BsiWI BspHI DraI EagI EclXI
Eco52I FseI HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NdeI NotI NruI PacI
PinAI PmeI PshAI Psp1406I PvuI
RcaI SacII SbfI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 5825 ACATGT A^C[GA][CT]GT
Alw44I 52 GTGCAC G^TGCAC
ApaLI 52 GTGCAC G^TGCAC
AscI 6980 GGCGCGCC GG^CGCGCC
AseI 5140 ATTAAT AT^TAAT
AsnI 5140 ATTAAT AT^TAAT
AspI 5233 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 6749 TGCGCA TGC^GCA
AvrII 3711 CCTAGG C^CTAGG
BclI 3401 TGATCA T^GATCA
BlnI 3711 CCTAGG C^CTAGG
Bsp1407I 2027 TGTACA T^GTACA
BspDI 6877 ATCGAT AT^CGAT
BspLU11I 5825 ACATGT A^CATGT
BsrGI 2027 TGTACA T^GTACA
BssHII 6981 GCGCGC G^CGCGC
Bst1107I 4482 GTATAC GTA^TAC
BstBI 1306 TTCGAA TT^CGAA
BstZ17I 4482 GTATAC GTA^TAC
ClaI 6877 ATCGAT AT^CGAT
CpoI 2623 CGGACCG CG^G[AT]CCG
EcoRV 5590 GATATC GAT^ATC
EcoT22I 5517 ATGCAT ATGCA^T
FbaI 3401 TGATCA T^GATCA
FspI 6749 TGCGCA TGC^GCA
HindIII 5354 AAGCTT A^AGCTT
NsiI 5517 ATGCAT ATGCA^T
NspV 1306 TTCGAA TT^CGAA
PflFI 5233 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5140 ATTAAT AT^TAAT
RsrII 2623 CGGACCG CG^G[AT]CCG
SalI 6037 GTCGAC G^TCGAC
ScaI 4411 AGTACT AGT^ACT
SexAI 3726 ACCAGGT A^CC[AT]GGT
SfuI 1306 TTCGAA TT^CGAA
SspBI 2027 TGTACA T^GTACA
Tth111I 5233 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 2524 GACGTC GACGT^C
6838 GACGTC
AccI 4482 GTATAC GT^[AC][GT]AC
6037 GTCGAC
AhdI 4015 GACTCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6409 GACCATAAGTC
Aor51HI 3263 AGCGCT AGC^GCT
6254 AGCGCT
AspEI 4015 GACTCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6409 GACCATAAGTC
BalI 3395 TGGCCA TGG^CCA
6329 TGGCCA
BamHI 4837 GGATCC G^GATCC
6996 GGATCC
BbrPI 76 CACGTG CAC^GTG
6523 CACGTG
BglII 266 AGATCT A^GATCT
6542 AGATCT
BsaAI 76 CACGTG [CT]AC^GT[GA]
6523 CACGTG
BsmI 5515 GAATGC GAATGC(1/-1)
5848 GAATGC
BssSI 922 CACGAG CACGAG(-5/-1)
7051 CACGAG
BstEII 4006 GGTAACC G^GT[ACGT]ACC
7162 GGTGACC
BstPI 4006 GGTAACC G^GT[ACGT]ACC
7162 GGTGACC
DraIII 4987 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
6520 CACCACGTG
Eam1105I 4015 GACTCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6409 GACCATAAGTC
Eco47III 3263 AGCGCT AGC^GCT
6254 AGCGCT
EcoO65I 4006 GGTAACC G^GT[ACGT]ACC
7162 GGTGACC
MluNI 3395 TGGCCA TGG^CCA
6329 TGGCCA
MscI 3395 TGGCCA TGG^CCA
6329 TGGCCA
NheI 4098 GCTAGC G^CTAGC
4390 GCTAGC
PaeR7I 2417 CTCGAG C^TCGAG
6891 CTCGAG
PmaCI 76 CACGTG CAC^GTG
6523 CACGTG
PmlI 76 CACGTG CAC^GTG
6523 CACGTG
SphI 1340 GCATGC GCATG^C
1712 GCATGC
XhoI 2417 CTCGAG C^TCGAG
6891 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI