################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 1648 CGCG CG^CG AcyI 806 GGCGTC G[GA]^CG[CT]C AfaI 114 GTAC GT^AC AlwNI 623 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 1098 GAGCTC G[AT]GC[AT]^C AspI 1773 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 855 CTCGGG C^[CT]CG[GA]G BanI 1259 GGCACC G^G[CT][GA]CC BanII 1098 GAGCTC G[GA]GC[CT]^C BbsI 1807 GTCTTC GAAGAC(2/6) BcgI 1227 GCACAGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 356 AGATCT A^GATCT BpuAI 1807 GTCTTC GAAGAC(2/6) BsaHI 806 GGCGTC G[GA]^CG[CT]C BsaI 150 GGTCTC GGTCTC(1/5) BsgI 876 GTGCAG GTGCAG(16/14) BsiHKAI 1098 GAGCTC G[AT]GC[AT]^C BsmFI 660 GGGAC GGGAC(10/14) BsmI 715 GAATGC GAATGC(1/-1) BsoBI 855 CTCGGG C^[CT]CG[GA]G Bsp1407I 113 TGTACA T^GTACA BsrGI 113 TGTACA T^GTACA Bst1107I 903 GTATAC GTA^TAC BstUI 1648 CGCG CG^CG BstXI 517 CCAGTAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 356 AGATCT [GA]^GATC[CT] BstZ17I 903 GTATAC GTA^TAC DraIII 292 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 271 GAATTC G^AATTC EcoT14I 214 CCTTGG C^C[AT][AT]GG Hin1I 806 GGCGTC G[GA]^CG[CT]C MflI 356 AGATCT [GA]^GATC[CT] MvnI 1648 CGCG CG^CG NlaIII 703 CATG CATG^ PflFI 1773 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 291 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 106 AGGACCC [GA]G^G[AT]CC[CT] PshAI 244 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 193 CAGCTG CAG^CTG RsaI 114 GTAC GT^AC SacI 1098 GAGCTC GAGCT^C SmlI 1132 CTTGAG C^T[CT][GA]AG SnaBI 1543 TACGTA TAC^GTA SspBI 113 TGTACA T^GTACA StuI 824 AGGCCT AGG^CCT StyI 214 CCTTGG C^C[AT][AT]GG TaqI 188 TCGA T^CGA TspRI 1303 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 1773 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 188 TCGA T^CGA Van91I 291 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 356 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 903 GTATAC GT^[AC][GT]AC 1546 GTAGAC AcsI 271 GAATTC [GA]^AATT[CT] 479 GAATTT ApoI 271 GAATTC [GA]^AATT[CT] 479 GAATTT Asp700I 232 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1032 GAAGCCCTTC BalI 342 TGGCCA TGG^CCA 1572 TGGCCA BbvI 315 GCAGC GCAGC(8/12) 1155 GCAGC BsaAI 641 TACGTG [CT]AC^GT[GA] 1543 TACGTA Cac8I 311 GCTCGC GC[ACGT]^[ACGT]GC 1479 GCGTGC CfoI 334 GCGC GCG^C 1647 GCGC DpnII 357 GATC ^GATC 740 GATC EaeI 342 TGGCCA [CT]^GGCC[GA] 1572 TGGCCA FauI 719 GCGGG CCCGC(4/6) 1311 GCGGG HgaI 1017 GACGC GACGC(5/10) 807 GCGTC HhaI 334 GCGC GCG^C 1647 GCGC HinP1I 334 GCGC G^CGC 1647 GCGC MaeII 642 ACGT A^CGT 1544 ACGT MboI 357 GATC ^GATC 740 GATC MluNI 342 TGGCCA TGG^CCA 1572 TGGCCA MscI 342 TGGCCA TGG^CCA 1572 TGGCCA MslI 505 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 785 CATACCTGTG MspA1I 193 CAGCTG C[AC]G^C[GT]G 1684 CAGCGG NdeII 357 GATC ^GATC 740 GATC PleI 11 GACTC GAGTC(4/5) 579 GAGTC PstI 374 CTGCAG CTGCA^G 1716 CTGCAG Sau3AI 357 GATC ^GATC 740 GATC SexAI 293 ACCTGGT A^CC[AT]GGT 1178 ACCAGGT TfiI 625 GAATC G^A[AT]TC 933 GATTC TseI 315 GCAGC G^C[AT]GC 1155 GCAGC XmnI 232 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1032 GAAGCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI AvaII BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI Cfr13I DdeI DraII EcoO109I EcoO65I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboII MnlI MseI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI SfaNI SfcI Tru9I Tsp45I Tsp509I