Restriction map for
pF1KA1840
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# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AspEI AvrII BbrPI BlnI
BsiEI BsiWI BsmBI BspDI BsrBI
BssHII Bst1107I BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI MluI NotI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SbfI
SfiI SnaBI Sse8387I SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 6589 GGTACC G^GTACC
AccIII 6059 TCCGGA T^CCGGA
AflIII 731 ACATGT A^C[GA][CT]GT
Alw44I 3399 GTGCAC G^TGCAC
ApaI 243 GGGCCC GGGCC^C
ApaLI 3399 GTGCAC G^TGCAC
Asp700I 907 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 6589 GGTACC G^GTACC
AspI 7174 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 128 CCCGGG C^[CT]CG[GA]G
AviII 140 TGCGCA TGC^GCA
BbeI 5648 GGCGCC GGCGC^C
BbvCI 7012 GCTGAGG CCTCAGC(-5/-2)
BcgI 6705 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 5812 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5812 GCTGAGC GC^T[ACGT]AGC
BsaAI 2303 TACGTG [CT]AC^GT[GA]
BsaBI 1897 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 6059 TCCGGA [AT]^CCGG[AT]
BseAI 6059 TCCGGA T^CCGGA
BsoBI 128 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2007 TGTACA T^GTACA
BspEI 6059 TCCGGA T^CCGGA
BspLU11I 731 ACATGT A^CATGT
BspMI 7324 GCAGGT ACCTGC(4/8)
BsrGI 2007 TGTACA T^GTACA
BstBI 1949 TTCGAA TT^CGAA
BstEII 1123 GGTAACC G^GT[ACGT]ACC
BstPI 1123 GGTAACC G^GT[ACGT]ACC
CelII 5812 GCTGAGC GC^T[ACGT]AGC
EcoO65I 1123 GGTAACC G^GT[ACGT]ACC
EcoRI 632 GAATTC G^AATTC
EcoRV 436 GATATC GAT^ATC
FspI 140 TGCGCA TGC^GCA
HgaI 204 GACGC GACGC(5/10)
HpaI 955 GTTAAC GTT^AAC
KasI 5648 GGCGCC G^GCGCC
KpnI 6589 GGTACC GGTAC^C
KspI 56 CCGCGG CCGC^GG
MamI 1897 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 6059 TCCGGA T^CCGGA
NaeI 37 GCCGGC GCC^GGC
NarI 5648 GGCGCC GG^CGCC
NdeI 692 CATATG CA^TATG
NgoMIV 37 GCCGGC G^CCGGC
NruI 20 TCGCGA TCG^CGA
NspV 1949 TTCGAA TT^CGAA
PflFI 7174 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2441 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 6349 GAGCTC GAGCT^C
SacII 56 CCGCGG CCGC^GG
SexAI 3557 ACCTGGT A^CC[AT]GGT
SfoI 5648 GGCGCC GGC^GCC
SfuI 1949 TTCGAA TT^CGAA
SgrAI 36 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 128 CCCGGG CCC^GGG
SpeI 5482 ACTAGT A^CTAGT
SphI 1376 GCATGC GCATG^C
SspBI 2007 TGTACA T^GTACA
Tth111I 7174 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2441 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 128 CCCGGG C^CCGGG
XmaI 128 CCCGGG C^CCGGG
XmnI 907 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 21 CGCG CG^CG
57 CGCG
AcyI 204 GACGCC G[GA]^CG[CT]C
5648 GGCGCC
BamHI 3333 GGATCC G^GATCC
6579 GGATCC
BbsI 727 GAAGAC GAAGAC(2/6)
2863 GAAGAC
BciVI 2916 GGATAC GTATCC(6/5)
4950 GGATAC
BclI 1695 TGATCA T^GATCA
1704 TGATCA
BglI 236 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5266 GCCCATGTGGC
BpuAI 727 GAAGAC GAAGAC(2/6)
2863 GAAGAC
BsaHI 204 GACGCC G[GA]^CG[CT]C
5648 GGCGCC
BsaI 1366 GAGACC GGTCTC(1/5)
1427 GAGACC
BsmI 2872 GAATGC GAATGC(1/-1)
3977 GCATTC
BspHI 1514 TCATGA T^CATGA
1958 TCATGA
BsrFI 37 GCCGGC [GA]^CCGG[CT]
5693 GCCGGT
BssSI 6703 CACGAG CACGAG(-5/-1)
6154 CTCGTG
BstUI 21 CGCG CG^CG
57 CGCG
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
5693 GCCGGT
DraIII 1398 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
6261 CACTCTGTG
EcoT22I 1174 ATGCAT ATGCA^T
3521 ATGCAT
FbaI 1695 TGATCA T^GATCA
1704 TGATCA
Hin1I 204 GACGCC G[GA]^CG[CT]C
5648 GGCGCC
MfeI 1825 CAATTG C^AATTG
2263 CAATTG
MunI 1825 CAATTG C^AATTG
2263 CAATTG
MvnI 21 CGCG CG^CG
57 CGCG
NheI 3166 GCTAGC G^CTAGC
7320 GCTAGC
NsiI 1174 ATGCAT ATGCA^T
3521 ATGCAT
RcaI 1514 TCATGA T^CATGA
1958 TCATGA
SalI 3119 GTCGAC G^TCGAC
5972 GTCGAC
ScaI 558 AGTACT AGT^ACT
4178 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaII BalI
BanI BanII BbvI BcnI BfaI
BfrI BglII BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII