Restriction map for
pF1KA2004
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BseRI BsiEI BsiWI BsoBI Bsp1407I
BspDI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MluI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 3118 CGCG CG^CG
AccIII 2702 TCCGGA T^CCGGA
AflIII 478 ACATGT A^C[GA][CT]GT
Alw44I 1732 GTGCAC G^TGCAC
ApaLI 1732 GTGCAC G^TGCAC
BanI 2881 GGCACC G^G[CT][GA]CC
BbvI 1123 GCAGC GCAGC(8/12)
BclI 2657 TGATCA T^GATCA
BglII 1761 AGATCT A^GATCT
BsaBI 52 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2702 TCCGGA [AT]^CCGG[AT]
BseAI 2702 TCCGGA T^CCGGA
BsgI 2162 GTGCAG GTGCAG(16/14)
BsmBI 1432 GAGACG CGTCTC(1/5)
BspEI 2702 TCCGGA T^CCGGA
BspLU11I 478 ACATGT A^CATGT
BspMI 1487 GCAGGT ACCTGC(4/8)
BstEII 2328 GGTAACC G^GT[ACGT]ACC
BstPI 2328 GGTAACC G^GT[ACGT]ACC
BstUI 3118 CGCG CG^CG
BstXI 1528 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2807 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 820 GACCATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 729 CCATGG C^C[GA][CT]GG
EcoNI 547 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2328 GGTAACC G^GT[ACGT]ACC
EcoRV 1939 GATATC GAT^ATC
FauI 4663 CCCGC CCCGC(4/6)
FbaI 2657 TGATCA T^GATCA
Fnu4HI 1123 GCAGC GC^[ACGT]GC
HapII 2703 CCGG C^CGG
HincII 4676 GTCAAC GT[CT]^[GA]AC
HindII 4676 GTCAAC GT[CT]^[GA]AC
HindIII 1358 AAGCTT A^AGCTT
HpaII 2703 CCGG C^CGG
ItaI 1123 GCAGC GC^[ACGT]GC
MamI 52 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2702 TCCGGA T^CCGGA
MspA1I 1467 CAGCTG C[AC]G^C[GT]G
MspI 2703 CCGG C^CGG
MvnI 3118 CGCG CG^CG
NcoI 729 CCATGG C^CATGG
PstI 2428 CTGCAG CTGCA^G
PvuII 1467 CAGCTG CAG^CTG
SacI 421 GAGCTC GAGCT^C
SapI 2764 GCTCTTC GCTCTTC(1/4)
SnaBI 2371 TACGTA TAC^GTA
TseI 1123 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 539 GTCTAC GT^[AC][GT]AC
3376 GTCTAC
AseI 1779 ATTAAT AT^TAAT
4522 ATTAAT
AsnI 1779 ATTAAT AT^TAAT
4522 ATTAAT
AspHI 421 GAGCTC G[AT]GC[AT]^C
1732 GTGCAC
BalI 4169 TGGCCA TGG^CCA
4318 TGGCCA
BamHI 2537 GGATCC G^GATCC
3720 GGATCC
BanII 421 GAGCTC G[GA]GC[CT]^C
4036 GGGCTC
BpmI 3631 CTCCAG CTGGAG(16/14)
4203 CTCCAG
BsaAI 686 CACGTA [CT]AC^GT[GA]
2371 TACGTA
BsiHKAI 421 GAGCTC G[AT]GC[AT]^C
1732 GTGCAC
BspHI 1943 TCATGA T^CATGA
2660 TCATGA
BstAPI 3084 GCAAGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3466 GCAGAACATGC
EaeI 4169 TGGCCA [CT]^GGCC[GA]
4318 TGGCCA
EcoT22I 3242 ATGCAT ATGCA^T
4411 ATGCAT
MfeI 4430 CAATTG C^AATTG
4608 CAATTG
MluNI 4169 TGGCCA TGG^CCA
4318 TGGCCA
MscI 4169 TGGCCA TGG^CCA
4318 TGGCCA
MunI 4430 CAATTG C^AATTG
4608 CAATTG
NdeI 2777 CATATG CA^TATG
4408 CATATG
NsiI 3242 ATGCAT ATGCA^T
4411 ATGCAT
NspI 478 ACATGT [GA]CATG^[CT]
3471 ACATGC
PshBI 1779 ATTAAT AT^TAAT
4522 ATTAAT
RcaI 1943 TCATGA T^CATGA
2660 TCATGA
ScaI 889 AGTACT AGT^ACT
2432 AGTACT
SmlI 3477 CTCAAG C^T[CT][GA]AG
4370 CTCAAG
SspI 2725 AATATT AAT^ATT
3847 AATATT
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbsI BciVI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I FokI
HaeIII HinfI HphI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI