Restriction map for
pF1KA2014
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbrPI
BclI BfrI BlnI BsaAI BsaBI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssSI Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KspI MamI MfeI MluI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 667 GACGTC GACGT^C
Acc65I 2971 GGTACC G^GTACC
AccII 587 CGCG CG^CG
AcsI 379 GAATTT [GA]^AATT[CT]
AflIII 1130 ACGTGT A^C[GA][CT]GT
AocI 1534 CCTGAGG CC^T[ACGT]AGG
ApoI 379 GAATTT [GA]^AATT[CT]
Asp700I 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2971 GGTACC G^GTACC
BbeI 2927 GGCGCC GGCGC^C
BcgI 515 GCAGGTCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 123 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC
BsaI 1165 GAGACC GGTCTC(1/5)
BspHI 701 TCATGA T^CATGA
BsrDI 2250 CATTGC GCAATG(2/0)
BsrFI 2945 ACCGGC [GA]^CCGG[CT]
BssHII 586 GCGCGC G^CGCGC
BstBI 1676 TTCGAA TT^CGAA
BstUI 587 CGCG CG^CG
Bsu36I 1534 CCTGAGG CC^T[ACGT]AGG
CelII 123 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2945 ACCGGC [GA]^CCGG[CT]
Eco81I 1534 CCTGAGG CC^T[ACGT]AGG
HindIII 1231 AAGCTT A^AGCTT
KasI 2927 GGCGCC G^GCGCC
KpnI 2971 GGTACC GGTAC^C
MvnI 587 CGCG CG^CG
NarI 2927 GGCGCC GG^CGCC
NdeI 1328 CATATG CA^TATG
NspV 1676 TTCGAA TT^CGAA
PshAI 1603 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 701 TCATGA T^CATGA
SacI 126 GAGCTC GAGCT^C
SapI 2145 GCTCTTC GCTCTTC(1/4)
ScaI 324 AGTACT AGT^ACT
SfoI 2927 GGCGCC GGC^GCC
SfuI 1676 TTCGAA TT^CGAA
SmaI 30 CCCGGG CCC^GGG
SspI 946 AATATT AAT^ATT
XcmI 2172 CCAGCGAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
XmnI 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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AccIII 929 TCCGGA T^CCGGA
2609 TCCGGA
Alw44I 991 GTGCAC G^TGCAC
1351 GTGCAC
ApaI 629 GGGCCC GGGCC^C
1845 GGGCCC
ApaLI 991 GTGCAC G^TGCAC
1351 GTGCAC
AvaI 30 CCCGGG C^[CT]CG[GA]G
3062 CTCGGG
BalI 1457 TGGCCA TGG^CCA
1940 TGGCCA
BanI 2927 GGCGCC G^G[CT][GA]CC
2971 GGTACC
BciVI 2391 GGATAC GTATCC(6/5)
680 GTATCC
BglI 547 GCCCTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2662 GCCAAGACGGC
BseAI 929 TCCGGA T^CCGGA
2609 TCCGGA
BsoBI 30 CCCGGG C^[CT]CG[GA]G
3062 CTCGGG
BspEI 929 TCCGGA T^CCGGA
2609 TCCGGA
BstAPI 1353 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2481 GCATGAGCTGC
DraIII 994 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1345 CACCAGGTG
DrdI 667 GACGTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2596 GACAACAGCGTC
EcoT14I 787 CCAAGG C^C[AT][AT]GG
2936 CCAAGG
MluNI 1457 TGGCCA TGG^CCA
1940 TGGCCA
MroI 929 TCCGGA T^CCGGA
2609 TCCGGA
MscI 1457 TGGCCA TGG^CCA
1940 TGGCCA
MslI 739 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
984 CATCGTGGTG
SexAI 725 ACCTGGT A^CC[AT]GGT
1346 ACCAGGT
SphI 968 GCATGC GCATG^C
1490 GCATGC
StyI 787 CCAAGG C^C[AT][AT]GG
2936 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII