Restriction map for
pF1KB0051
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# NO CUT ENZYMES ( 58 enzymes) #
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AclI AcsI AflII AgeI Aor51HI
ApoI AscI AseI AsnI AviII
AvrII BamHI BclI BfrI BlnI
BsaBI BsiWI BsmI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eco47III EcoRI EcoRV
FbaI FseI FspI MamI MfeI
MunI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I RcaI RsrII SalI
ScaI SexAI SfuI SnaBI SpeI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 2779 GACGTC GACGT^C
AccI 3712 GTCTAC GT^[AC][GT]AC
Alw44I 5235 GTGCAC G^TGCAC
ApaLI 5235 GTGCAC G^TGCAC
BbrPI 4294 CACGTG CAC^GTG
BcgI 2475 CGACCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3722 AGATCT A^GATCT
Bsp1407I 5120 TGTACA T^GTACA
BsrDI 1067 GCAATG GCAATG(2/0)
BsrGI 5120 TGTACA T^GTACA
BssSI 1288 CACGAG CACGAG(-5/-1)
DraIII 2905 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
HindIII 144 AAGCTT A^AGCTT
HpaI 112 GTTAAC GTT^AAC
KspI 5247 CCGCGG CCGC^GG
MluI 200 ACGCGT A^CGCGT
MseI 113 TTAA T^TAA
NcoI 4569 CCATGG C^CATGG
PaeR7I 2670 CTCGAG C^TCGAG
PflMI 1953 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4294 CACGTG CAC^GTG
PmlI 4294 CACGTG CAC^GTG
PvuI 5024 CGATCG CGAT^CG
PvuII 1865 CAGCTG CAG^CTG
SacII 5247 CCGCGG CCGC^GG
SapI 3118 GAAGAGC GCTCTTC(1/4)
SfiI 2958 GGCCGTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4327 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 5120 TGTACA T^GTACA
TfiI 922 GATTC G^A[AT]TC
Tru9I 113 TTAA T^TAA
Van91I 1953 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2670 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Acc65I 2174 GGTACC G^GTACC
5042 GGTACC
Asp700I 2940 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3118 GAAGAGCTTC
Asp718I 2174 GGTACC G^GTACC
5042 GGTACC
AspI 2776 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3480 GACCAAGTC
BbeI 4331 GGCGCC GGCGC^C
5102 GGCGCC
BciVI 262 GGATAC GTATCC(6/5)
3979 GGATAC
BglI 1426 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2959 GCCGTCCAGGC
BsaAI 529 TACGTG [CT]AC^GT[GA]
4294 CACGTG
BspLU11I 410 ACATGT A^CATGT
5117 ACATGT
EagI 2808 CGGCCG C^GGCCG
3758 CGGCCG
EclXI 2808 CGGCCG C^GGCCG
3758 CGGCCG
Eco52I 2808 CGGCCG C^GGCCG
3758 CGGCCG
KasI 4331 GGCGCC G^GCGCC
5102 GGCGCC
KpnI 2174 GGTACC GGTAC^C
5042 GGTACC
NarI 4331 GGCGCC GG^CGCC
5102 GGCGCC
PflFI 2776 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3480 GACCAAGTC
SacI 1363 GAGCTC GAGCT^C
4279 GAGCTC
SbfI 3321 CCTGCAGG CCTGCA^GG
4455 CCTGCAGG
SfoI 4331 GGCGCC GGC^GCC
5102 GGCGCC
SphI 249 GCATGC GCATG^C
3782 GCATGC
Sse8387I 3321 CCTGCAGG CCTGCA^GG
4455 CCTGCAGG
StuI 3216 AGGCCT AGG^CCT
3395 AGGCCT
Tth111I 2776 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3480 GACCAAGTC
XmnI 2940 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3118 GAAGAGCTTC
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# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NsiI
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI