################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp718I AspI AvrII BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SnaBI SpeI Sse8387I SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 615 GACGTC GACGT^C AflIII 218 ACGTGT A^C[GA][CT]GT AhdI 949 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 40 GTGCAC G^TGCAC AocI 968 CCTCAGG CC^T[ACGT]AGG Aor51HI 1510 AGCGCT AGC^GCT ApaLI 40 GTGCAC G^TGCAC Asp700I 1488 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 949 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1574 TGCGCA TGC^GCA BalI 716 TGGCCA TGG^CCA BamHI 925 GGATCC G^GATCC BbrPI 217 CACGTG CAC^GTG BbsI 1711 GTCTTC GAAGAC(2/6) BbvCI 1424 CCTCAGC CCTCAGC(-5/-2) BpuAI 1711 GTCTTC GAAGAC(2/6) BsaAI 217 CACGTG [CT]AC^GT[GA] BsgI 725 GTGCAG GTGCAG(16/14) BsiEI 336 CGACCG CG[GA][CT]^CG BsmBI 1546 GAGACG CGTCTC(1/5) Bsp1407I 221 TGTACA T^GTACA BsrGI 221 TGTACA T^GTACA Bsu36I 968 CCTCAGG CC^T[ACGT]AGG DraIII 1163 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 716 TGGCCA [CT]^GGCC[GA] Eam1105I 949 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1510 AGCGCT AGC^GCT Eco81I 968 CCTCAGG CC^T[ACGT]AGG FspI 1574 TGCGCA TGC^GCA KspI 1774 CCGCGG CCGC^GG MaeIII 573 GTCAC ^GT[ACGT]AC MluNI 716 TGGCCA TGG^CCA MscI 716 TGGCCA TGG^CCA MslI 168 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1587 GCCGGC GCC^GGC NgoMIV 1587 GCCGGC G^CCGGC NspI 173 GCATGC [GA]CATG^[CT] PmaCI 217 CACGTG CAC^GTG PmlI 217 CACGTG CAC^GTG SacI 1761 GAGCTC GAGCT^C SacII 1774 CCGCGG CCGC^GG SexAI 1164 ACCTGGT A^CC[AT]GGT SgrAI 1586 CGCCGGCG C[GA]^CCGG[CT]G SmaI 536 CCCGGG CCC^GGG SphI 173 GCATGC GCATG^C SspBI 221 TGTACA T^GTACA TfiI 1207 GAATC G^A[AT]TC Tsp45I 573 GTCAC ^GT[GC]AC XmaCI 536 CCCGGG C^CCGGG XmaI 536 CCCGGG C^CCGGG XmnI 1488 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 1237 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1741 CAGCCGCTG AspHI 40 GTGCAC G[AT]GC[AT]^C 1761 GAGCTC BbeI 20 GGCGCC GGCGC^C 1620 GGCGCC BfaI 107 CTAG C^TAG 1354 CTAG BlpI 1665 GCTCAGC GC^T[ACGT]AGC 1763 GCTCAGC Bpu1102I 1665 GCTCAGC GC^T[ACGT]AGC 1763 GCTCAGC BsiHKAI 40 GTGCAC G[AT]GC[AT]^C 1761 GAGCTC BsrDI 236 CATTGC GCAATG(2/0) 86 GCAATG BstAPI 1236 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1740 GCAGCCGCTGC BstYI 925 GGATCC [GA]^GATC[CT] 1002 GGATCT CelII 1665 GCTCAGC GC^T[ACGT]AGC 1763 GCTCAGC HincII 361 GTCAAC GT[CT]^[GA]AC 1441 GTCAAC HindII 361 GTCAAC GT[CT]^[GA]AC 1441 GTCAAC KasI 20 GGCGCC G^GCGCC 1620 GGCGCC MaeI 107 CTAG C^TAG 1354 CTAG MflI 925 GGATCC [GA]^GATC[CT] 1002 GGATCT NarI 20 GGCGCC GG^CGCC 1620 GGCGCC NcoI 713 CCATGG C^CATGG 883 CCATGG SfoI 20 GGCGCC GGC^GCC 1620 GGCGCC TaqI 335 TCGA T^CGA 1177 TCGA TthHB8I 335 TCGA T^CGA 1177 TCGA XhoII 925 GGATCC [GA]^GATC[CT] 1002 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TseI TspRI XcmI