################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AlwNI 1403 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 549 GGACC G^G[AT]CC BalI 962 TGGCCA TGG^CCA BbrPI 1749 CACGTG CAC^GTG BsaAI 1749 CACGTG [CT]AC^GT[GA] BsgI 367 CTGCAC GTGCAG(16/14) BsrBI 394 CCGCTC CCGCTC(-3/-3) BstAPI 1822 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 655 TTCGAA TT^CGAA DraIII 1559 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 294 CGGCCG C^GGCCG EclXI 294 CGGCCG C^GGCCG Eco52I 294 CGGCCG C^GGCCG EcoRI 925 GAATTC G^AATTC HinfI 992 GACTC G^A[ACGT]TC KspI 261 CCGCGG CCGC^GG MluNI 962 TGGCCA TGG^CCA MscI 962 TGGCCA TGG^CCA NotI 293 GCGGCCGC GC^GGCCGC NspV 655 TTCGAA TT^CGAA PleI 992 GACTC GAGTC(4/5) PmaCI 1749 CACGTG CAC^GTG PmlI 1749 CACGTG CAC^GTG PpuMI 548 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1567 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1220 GAGCTC GAGCT^C SacII 261 CCGCGG CCGC^GG SapI 396 GCTCTTC GCTCTTC(1/4) ScaI 1132 AGTACT AGT^ACT SfuI 655 TTCGAA TT^CGAA SmlI 76 CTCAAG C^T[CT][GA]AG SphI 1349 GCATGC GCATG^C SspI 763 AATATT AAT^ATT XcmI 1301 CCATGACTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 325 GGCGTC G[GA]^CG[CT]C 481 GACGCC AocI 1038 CCTCAGG CC^T[ACGT]AGG 1501 CCTCAGG ApaI 597 GGGCCC GGGCC^C 647 GGGCCC BamHI 834 GGATCC G^GATCC 1061 GGATCC BanI 46 GGCACC G^G[CT][GA]CC 317 GGCACC BbsI 658 GAAGAC GAAGAC(2/6) 328 GTCTTC BglI 484 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 599 GCCCCGACGGC BpuAI 658 GAAGAC GAAGAC(2/6) 328 GTCTTC BsaHI 325 GGCGTC G[GA]^CG[CT]C 481 GACGCC BsaI 433 GAGACC GGTCTC(1/5) 508 GAGACC BsiEI 294 CGGCCG CG[GA][CT]^CG 312 CGACCG BsmFI 990 GGGAC GGGAC(10/14) 210 GTCCC BspLU11I 1570 ACATGT A^CATGT 1720 ACATGT BsrDI 1447 GCAATG GCAATG(2/0) 1826 GCAATG BstXI 282 CCATCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 560 CCAAGAGGCTGG BstYI 834 GGATCC [GA]^GATC[CT] 1061 GGATCC Bsu36I 1038 CCTCAGG CC^T[ACGT]AGG 1501 CCTCAGG DsaI 143 CCACGG C^C[GA][CT]GG 261 CCGCGG Eco81I 1038 CCTCAGG CC^T[ACGT]AGG 1501 CCTCAGG EcoT14I 1442 CCTTGG C^C[AT][AT]GG 1468 CCAAGG Hin1I 325 GGCGTC G[GA]^CG[CT]C 481 GACGCC HindIII 755 AAGCTT A^AGCTT 1797 AAGCTT MflI 834 GGATCC [GA]^GATC[CT] 1061 GGATCC SfaNI 425 GCATC GCATC(5/9) 572 GCATC SmaI 214 CCCGGG CCC^GGG 321 CCCGGG StyI 1442 CCTTGG C^C[AT][AT]GG 1468 CCAAGG XhoII 834 GGATCC [GA]^GATC[CT] 1061 GGATCC XmaCI 214 CCCGGG C^CCGGG 321 CCCGGG XmaI 214 CCCGGG C^CCGGG 321 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PstI RsaI Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I