################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AgeI AhdI AocI AscI Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HpaII KasI KpnI KspI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 112 GTAGAC GT^[AC][GT]AC AflII 2068 CTTAAG C^TTAAG AflIII 641 ACATGT A^C[GA][CT]GT Alw44I 701 GTGCAC G^TGCAC AlwNI 1534 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1515 AGCGCT AGC^GCT ApaI 363 GGGCCC GGGCC^C ApaLI 701 GTGCAC G^TGCAC AseI 478 ATTAAT AT^TAAT AsnI 478 ATTAAT AT^TAAT AvaI 2020 CTCGAG C^[CT]CG[GA]G AvaII 253 GGTCC G^G[AT]CC BfrI 2068 CTTAAG C^TTAAG BglI 1266 GCCTTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 188 TACGTG [CT]AC^GT[GA] BsaBI 1207 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 902 CCATGG C^C[ACGT][ACGT]GG BsgI 445 CTGCAC GTGCAG(16/14) BsmI 2144 GCATTC GAATGC(1/-1) BsoBI 2020 CTCGAG C^[CT]CG[GA]G BspLU11I 641 ACATGT A^CATGT BspMI 697 GCAGGT ACCTGC(4/8) BsrDI 89 GCAATG GCAATG(2/0) BstEII 2060 GGTCACC G^GT[ACGT]ACC BstPI 2060 GGTCACC G^GT[ACGT]ACC CfoI 1516 GCGC GCG^C DsaI 902 CCATGG C^C[GA][CT]GG EarI 376 GAAGAG CTCTTC(1/4) Eco47III 1515 AGCGCT AGC^GCT EcoO65I 2060 GGTCACC G^GT[ACGT]ACC EcoRV 1695 GATATC GAT^ATC EcoT14I 902 CCATGG C^C[AT][AT]GG HaeII 1515 AGCGCT [GA]GCGC^[CT] HhaI 1516 GCGC GCG^C HinP1I 1516 GCGC G^CGC Ksp632I 376 GAAGAG CTCTTC(1/4) MamI 1207 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1431 CAATTG C^AATTG MunI 1431 CAATTG C^AATTG NcoI 902 CCATGG C^CATGG PaeR7I 2020 CTCGAG C^TCGAG PleI 2023 GAGTC GAGTC(4/5) PshAI 511 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 478 ATTAAT AT^TAAT PstI 650 CTGCAG CTGCA^G SacI 1148 GAGCTC GAGCT^C SapI 376 GAAGAGC GCTCTTC(1/4) SpeI 1876 ACTAGT A^CTAGT SphI 381 GCATGC GCATG^C StyI 902 CCATGG C^C[AT][AT]GG XcmI 1785 CCAGTTTGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2020 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Asp700I 736 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1975 GAAGGATTTC AspHI 701 GTGCAC G[AT]GC[AT]^C 1148 GAGCTC BamHI 534 GGATCC G^GATCC 1233 GGATCC BsiHKAI 701 GTGCAC G[AT]GC[AT]^C 1148 GAGCTC BsiYI 366 CCCTTTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 902 CCATGGATCGG BslI 366 CCCTTTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 902 CCATGGATCGG BsmAI 334 GAGAC GTCTC(1/5) 20 GTCTC Bsp1407I 750 TGTACA T^GTACA 1104 TGTACA BsrGI 750 TGTACA T^GTACA 1104 TGTACA Cac8I 381 GCATGC GC[ACGT]^[ACGT]GC 652 GCAGGC DraII 362 AGGGCCC [GA]G^G[ACGT]CC[CT] 363 GGGCCCT EcoO109I 362 AGGGCCC [GA]G^G[ACGT]CC[CT] 363 GGGCCCT HincII 912 GTTAAC GT[CT]^[GA]AC 1390 GTTAAC HindII 912 GTTAAC GT[CT]^[GA]AC 1390 GTTAAC HindIII 1411 AAGCTT A^AGCTT 1951 AAGCTT HpaI 912 GTTAAC GTT^AAC 1390 GTTAAC HphI 508 GGTGA GGTGA(8/7) 2062 TCACC MaeII 189 ACGT A^CGT 600 ACGT MslI 636 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1396 CATTACTATG SfcI 110 CTGTAG C^T[GA][CT]AG 650 CTGCAG SspBI 750 TGTACA T^GTACA 1104 TGTACA SspI 1199 AATATT AAT^ATT 1301 AATATT TaqI 858 TCGA T^CGA 2021 TCGA TthHB8I 858 TCGA T^CGA 2021 TCGA XmnI 736 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1975 GAAGGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII