################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AlwNI AocI Aor51HI AscI AseI AsnI AspI AviII BbeI BbrPI BclI BfrI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KspI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AhdI 2599 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 745 GTGCAC G^TGCAC ApaI 3536 GGGCCC GGGCC^C ApaLI 745 GTGCAC G^TGCAC AspEI 2599 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2247 CCTAGG C^CTAGG BamHI 600 GGATCC G^GATCC BglI 2087 GCCACTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2532 AGATCT A^GATCT BlnI 2247 CCTAGG C^CTAGG BspDI 1725 ATCGAT AT^CGAT BspMI 3616 GCAGGT ACCTGC(4/8) ClaI 1725 ATCGAT AT^CGAT DraI 2673 TTTAAA TTT^AAA DraII 3535 GGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 1945 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2599 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3474 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 3535 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoT22I 1114 ATGCAT ATGCA^T HindIII 2453 AAGCTT A^AGCTT MfeI 1947 CAATTG C^AATTG MluI 2566 ACGCGT A^CGCGT MunI 1947 CAATTG C^AATTG NsiI 1114 ATGCAT ATGCA^T SacI 335 GAGCTC GAGCT^C SmaI 1164 CCCGGG CCC^GGG SpeI 1263 ACTAGT A^CTAGT SspI 377 AATATT AAT^ATT XmaCI 1164 CCCGGG C^CCGGG XmaI 1164 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Acc65I 257 GGTACC G^GTACC 550 GGTACC AccI 688 GTAGAC GT^[AC][GT]AC 1465 GTCTAC AccII 804 CGCG CG^CG 2567 CGCG Asp718I 257 GGTACC G^GTACC 550 GGTACC BcgI 1717 GCAGTGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4452 GCAGAAATCTCG BpmI 1334 CTGGAG CTGGAG(16/14) 4016 CTGGAG BsaBI 795 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4110 GATAGGCATC BspLU11I 2603 ACATGT A^CATGT 3603 ACATGT BstUI 804 CGCG CG^CG 2567 CGCG DraIII 1793 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 3505 CACCCAGTG FauI 2342 CCCGC CCCGC(4/6) 805 GCGGG HgaI 3556 GACGC GACGC(5/10) 3030 GCGTC KpnI 257 GGTACC GGTAC^C 550 GGTACC MamI 795 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4110 GATAGGCATC MvnI 804 CGCG CG^CG 2567 CGCG PstI 2864 CTGCAG CTGCA^G 3262 CTGCAG StuI 1856 AGGCCT AGG^CCT 3406 AGGCCT XcmI 1871 CCAACCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4013 CCACTGGAGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BmyI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI