Restriction map for
pF1KB3092
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# NO CUT ENZYMES ( 111 enzymes) #
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Acc65I AccII AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BciVI BcnI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsiEI
BsiWI Bsp1407I BspDI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRV FseI FspI HgaI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 476 GACGTC GACGT^C
AccI 486 GTCTAC GT^[AC][GT]AC
AcyI 476 GACGTC G[GA]^CG[CT]C
AflII 180 CTTAAG C^TTAAG
AhdI 471 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 971 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 696 CCTAAGG CC^T[ACGT]AGG
Asp700I 649 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 471 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 424 TGGCCA TGG^CCA
BamHI 1042 GGATCC G^GATCC
BcgI 14 CGAGCACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1757 CTAG C^TAG
BfrI 180 CTTAAG C^TTAAG
BglI 1695 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 411 AGATCT A^GATCT
BsaHI 476 GACGTC G[GA]^CG[CT]C
BseRI 1810 GAGGAG GAGGAG(10/8)
BsgI 302 CTGCAC GTGCAG(16/14)
BsmBI 478 CGTCTC CGTCTC(1/5)
BsmI 1116 GAATGC GAATGC(1/-1)
BsrDI 1666 GCAATG GCAATG(2/0)
Bsu36I 696 CCTAAGG CC^T[ACGT]AGG
CfoI 523 GCGC GCG^C
DsaI 1696 CCACGG C^C[GA][CT]GG
EaeI 424 TGGCCA [CT]^GGCC[GA]
Eam1105I 471 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 696 CCTAAGG CC^T[ACGT]AGG
HaeII 522 AGCGCC [GA]GCGC^[CT]
HhaI 523 GCGC GCG^C
Hin1I 476 GACGTC G[GA]^CG[CT]C
HinP1I 523 GCGC G^CGC
MaeI 1757 CTAG C^TAG
MluNI 424 TGGCCA TGG^CCA
MscI 424 TGGCCA TGG^CCA
NdeI 2012 CATATG CA^TATG
PaeR7I 1433 CTCGAG C^TCGAG
PpuMI 185 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1715 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 806 CTGCAG CTGCA^G
SacI 389 GAGCTC GAGCT^C
SapI 391 GCTCTTC GCTCTTC(1/4)
ScaI 419 AGTACT AGT^ACT
Tsp45I 835 GTCAC ^GT[GC]AC
XcmI 1526 CCATGAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1433 CTCGAG C^TCGAG
XmnI 649 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 782 TCCGGA T^CCGGA
1453 TCCGGA
AflIII 846 ACATGT A^C[GA][CT]GT
1716 ACATGT
AspHI 15 GAGCAC G[AT]GC[AT]^C
389 GAGCTC
AvaII 186 GGACC G^G[AT]CC
964 GGACC
BanII 389 GAGCTC G[GA]GC[CT]^C
1760 GAGCCC
BclI 222 TGATCA T^GATCA
1404 TGATCA
BpmI 481 CTCCAG CTGGAG(16/14)
1537 CTGGAG
BsaWI 782 TCCGGA [AT]^CCGG[AT]
1453 TCCGGA
BseAI 782 TCCGGA T^CCGGA
1453 TCCGGA
BsiHKAI 15 GAGCAC G[AT]GC[AT]^C
389 GAGCTC
BspEI 782 TCCGGA T^CCGGA
1453 TCCGGA
BspHI 590 TCATGA T^CATGA
1623 TCATGA
BspLU11I 846 ACATGT A^CATGT
1716 ACATGT
BstEII 1521 GGTTACC G^GT[ACGT]ACC
1893 GGTTACC
BstPI 1521 GGTTACC G^GT[ACGT]ACC
1893 GGTTACC
Cac8I 1078 GCCAGC GC[ACGT]^[ACGT]GC
1770 GCTTGC
DraII 185 GGGACCT [GA]G^G[ACGT]CC[CT]
1566 GGGGCCT
EcoO109I 185 GGGACCT [GA]G^G[ACGT]CC[CT]
1566 GGGGCCT
EcoO65I 1521 GGTTACC G^GT[ACGT]ACC
1893 GGTTACC
EcoRI 895 GAATTC G^AATTC
950 GAATTC
EcoT14I 189 CCTTGG C^C[AT][AT]GG
1342 CCAAGG
EcoT22I 626 ATGCAT ATGCA^T
1183 ATGCAT
FauI 552 GCGGG CCCGC(4/6)
1006 GCGGG
FbaI 222 TGATCA T^GATCA
1404 TGATCA
HapII 783 CCGG C^CGG
1454 CCGG
HindIII 1918 AAGCTT A^AGCTT
2072 AAGCTT
HpaII 783 CCGG C^CGG
1454 CCGG
HphI 618 TCACC GGTGA(8/7)
836 TCACC
MroI 782 TCCGGA T^CCGGA
1453 TCCGGA
MslI 127 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
619 CACCAAGATG
MspI 783 CCGG C^CGG
1454 CCGG
NsiI 626 ATGCAT ATGCA^T
1183 ATGCAT
PflMI 970 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1705 CCAACACTTGG
PleI 2008 GACTC GAGTC(4/5)
48 GAGTC
RcaI 590 TCATGA T^CATGA
1623 TCATGA
SfcI 806 CTGCAG C^T[GA][CT]AG
1935 CTACAG
StyI 189 CCTTGG C^C[AT][AT]GG
1342 CCAAGG
Van91I 970 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1705 CCAACACTTGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI BbvI BmyI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII