Restriction map for
pF1KB3114
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbvCI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 148 GTCGAC GT^[AC][GT]AC
AciI 175 GCGG CCGC(-3/-1)
AflIII 992 ACATGT A^C[GA][CT]GT
AlwI 951 GGATC GGATC(4/5)
AocI 334 CCTGAGG CC^T[ACGT]AGG
Asp700I 1491 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 238 GAGCTC G[AT]GC[AT]^C
BanII 238 GAGCTC G[GA]GC[CT]^C
BbrPI 874 CACGTG CAC^GTG
BcgI 486 CGATGTATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 225 GGATAC GTATCC(6/5)
BcnI 279 CCGGG CC^[GC]GG
BmyI 238 GAGCTC G[AGT]GC[ACT]^C
BpmI 945 CTGGAG CTGGAG(16/14)
BsaAI 874 CACGTG [CT]AC^GT[GA]
BseRI 1156 GAGGAG GAGGAG(10/8)
BsiHKAI 238 GAGCTC G[AT]GC[AT]^C
BsmAI 1624 GAGAC GTCTC(1/5)
Bsp1286I 238 GAGCTC G[AGT]GC[ACT]^C
BspHI 774 TCATGA T^CATGA
BspLU11I 992 ACATGT A^CATGT
BstXI 1382 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 951 GGATCT [GA]^GATC[CT]
Bsu36I 334 CCTGAGG CC^T[ACGT]AGG
CfoI 87 GCGC GCG^C
DraII 1450 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 334 CCTGAGG CC^T[ACGT]AGG
EcoNI 1149 CCTAATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1450 GGGTCCT [GA]G^G[ACGT]CC[CT]
HaeII 86 AGCGCC [GA]GCGC^[CT]
HapII 279 CCGG C^CGG
HhaI 87 GCGC GCG^C
HinP1I 87 GCGC G^CGC
HindIII 614 AAGCTT A^AGCTT
HpaI 139 GTTAAC GTT^AAC
HpaII 279 CCGG C^CGG
MflI 951 GGATCT [GA]^GATC[CT]
MspI 279 CCGG C^CGG
NciI 279 CCGGG CC^[GC]GG
NdeI 418 CATATG CA^TATG
PflMI 1112 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 874 CACGTG CAC^GTG
PmlI 874 CACGTG CAC^GTG
PpuMI 1450 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1168 CTGCAG CTGCA^G
RcaI 774 TCATGA T^CATGA
SacI 238 GAGCTC GAGCT^C
SalI 148 GTCGAC G^TCGAC
SbfI 1167 CCTGCAGG CCTGCA^GG
SexAI 1508 ACCTGGT A^CC[AT]GGT
SmlI 862 CTTGAG C^T[CT][GA]AG
SphI 366 GCATGC GCATG^C
Sse8387I 1167 CCTGCAGG CCTGCA^GG
SspI 163 AATATT AAT^ATT
Van91I 1112 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 951 GGATCT [GA]^GATC[CT]
XmnI 1491 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 402 GTAC GT^AC
639 GTAC
AlwNI 85 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
440 CAGCAGCTG
AspI 259 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
819 GACAAGGTC
BbsI 215 GAAGAC GAAGAC(2/6)
1591 GAAGAC
BfaI 8 CTAG C^TAG
964 CTAG
BpuAI 215 GAAGAC GAAGAC(2/6)
1591 GAAGAC
BsaJI 1142 CCATGG C^C[ACGT][ACGT]GG
1232 CCATGG
BsgI 1556 CTGCAC GTGCAG(16/14)
881 GTGCAG
BspDI 202 ATCGAT AT^CGAT
1261 ATCGAT
BsrDI 1401 CATTGC GCAATG(2/0)
751 GCAATG
BstF5I 317 GGATG GGATG(2/0)
465 GGATG
BstNI 1118 CCTGG CC^[AT]GG
1509 CCTGG
Cac8I 366 GCATGC GC[ACGT]^[ACGT]GC
1369 GCAAGC
ClaI 202 ATCGAT AT^CGAT
1261 ATCGAT
DsaI 1142 CCATGG C^C[GA][CT]GG
1232 CCATGG
EarI 1289 CTCTTC CTCTTC(1/4)
1491 GAAGAG
EcoRII 1118 CCTGG ^CC[AT]GG
1509 CCTGG
EcoT14I 1142 CCATGG C^C[AT][AT]GG
1232 CCATGG
FokI 317 GGATG GGATG(9/13)
465 GGATG
HaeIII 1146 GGCC GG^CC
1239 GGCC
Ksp632I 1289 CTCTTC CTCTTC(1/4)
1491 GAAGAG
MaeI 8 CTAG C^TAG
964 CTAG
MaeII 143 ACGT A^CGT
875 ACGT
MvaI 1118 CCTGG CC^[AT]GG
1509 CCTGG
NcoI 1142 CCATGG C^CATGG
1232 CCATGG
NspI 366 GCATGC [GA]CATG^[CT]
992 ACATGT
PflFI 259 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
819 GACAAGGTC
PleI 259 GACTC GAGTC(4/5)
1300 GACTC
RsaI 402 GTAC GT^AC
639 GTAC
SfcI 986 CTACAG C^T[GA][CT]AG
1168 CTGCAG
StyI 1142 CCATGG C^C[AT][AT]GG
1232 CCATGG
Tth111I 259 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
819 GACAAGGTC
XcmI 1108 CCAGCCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1142 CCATGGCCCTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BsiYI BslI BsmFI BsrI BssKI
Cfr13I DdeI DpnII DraIII Fnu4HI
HincII HindII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MwoI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I