Restriction map for
pF1KB3206
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI Asp718I AspEI AvrII
BalI BamHI BbvCI BcgI BfrI
BglI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoRV EcoT22I FseI HindIII HpaI
KpnI KspI MamI MflI MluI
MluNI MroI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflMI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Van91I XbaI XhoII
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1327 GACGTC GACGT^C
AccI 2062 GTCTAC GT^[AC][GT]AC
AlwNI 1082 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2187 GGGCCC GGGCC^C
Asp700I 856 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1871 TGCGCA TGC^GCA
BbeI 1594 GGCGCC GGCGC^C
BbrPI 1975 CACGTG CAC^GTG
BciVI 1106 GGATAC GTATCC(6/5)
BclI 381 TGATCA T^GATCA
BfaI 268 CTAG C^TAG
BlpI 1887 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1887 GCTGAGC GC^T[ACGT]AGC
BsaAI 1975 CACGTG [CT]AC^GT[GA]
BsmBI 789 CGTCTC CGTCTC(1/5)
BsrBI 120 GAGCGG CCGCTC(-3/-3)
BstAPI 36 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1755 GGTGACC G^GT[ACGT]ACC
BstPI 1755 GGTGACC G^GT[ACGT]ACC
BstXI 1760 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1887 GCTGAGC GC^T[ACGT]AGC
DpnII 382 GATC ^GATC
EagI 1736 CGGCCG C^GGCCG
EclXI 1736 CGGCCG C^GGCCG
Eco52I 1736 CGGCCG C^GGCCG
EcoNI 677 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1755 GGTGACC G^GT[ACGT]ACC
EcoRI 1001 GAATTC G^AATTC
FbaI 381 TGATCA T^GATCA
FspI 1871 TGCGCA TGC^GCA
HincII 1456 GTCAAC GT[CT]^[GA]AC
HindII 1456 GTCAAC GT[CT]^[GA]AC
KasI 1594 GGCGCC G^GCGCC
MaeI 268 CTAG C^TAG
MboI 382 GATC ^GATC
MfeI 932 CAATTG C^AATTG
MslI 1308 CATAACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 932 CAATTG C^AATTG
NarI 1594 GGCGCC GG^CGCC
NdeII 382 GATC ^GATC
NlaIII 847 CATG CATG^
PaeR7I 160 CTCGAG C^TCGAG
PflFI 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1975 CACGTG CAC^GTG
PmlI 1975 CACGTG CAC^GTG
Sau3AI 382 GATC ^GATC
SbfI 1569 CCTGCAGG CCTGCA^GG
SfoI 1594 GGCGCC GGC^GCC
SmaI 1834 CCCGGG CCC^GGG
Sse8387I 1569 CCTGCAGG CCTGCA^GG
Tth111I 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1907 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 160 CTCGAG C^TCGAG
XmaCI 1834 CCCGGG C^CCGGG
XmaI 1834 CCCGGG C^CCGGG
XmnI 856 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 1545 CCTCAGG CC^T[ACGT]AGG
2182 CCTGAGG
AseI 703 ATTAAT AT^TAAT
880 ATTAAT
AsnI 703 ATTAAT AT^TAAT
880 ATTAAT
BbsI 177 GTCTTC GAAGAC(2/6)
394 GTCTTC
BpuAI 177 GTCTTC GAAGAC(2/6)
394 GTCTTC
BseRI 277 GAGGAG GAGGAG(10/8)
2145 GAGGAG
BspMI 1490 ACCTGC ACCTGC(4/8)
2009 ACCTGC
Bsu36I 1545 CCTCAGG CC^T[ACGT]AGG
2182 CCTGAGG
DsaI 1605 CCACGG C^C[GA][CT]GG
1826 CCACGG
Eco81I 1545 CCTCAGG CC^T[ACGT]AGG
2182 CCTGAGG
HgaI 1657 GACGC GACGC(5/10)
1900 GACGC
PshBI 703 ATTAAT AT^TAAT
880 ATTAAT
PvuII 226 CAGCTG CAG^CTG
1813 CAGCTG
SapI 607 GAAGAGC GCTCTTC(1/4)
2097 GCTCTTC
SexAI 1751 ACCTGGT A^CC[AT]GGT
2066 ACCTGGT
SfaNI 764 GCATC GCATC(5/9)
1415 GCATC
SmlI 160 CTCGAG C^T[CT][GA]AG
1819 CTCAAG
TaqI 161 TCGA T^CGA
2348 TCGA
TfiI 488 GAATC G^A[AT]TC
750 GATTC
TthHB8I 161 TCGA T^CGA
2348 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV PleI
PpuMI PstI RsaI SacI Sau96I
ScrFI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI