Restriction map for
pF1KB3224
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspHI AvaI AvrII
BanI BbeI BbrPI BbsI BcnI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BssHII BssSI BstAPI
BstBI Bsu36I ClaI CpoI DraII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI FauI
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccI 201 GTATAC GT^[AC][GT]AC
AccII 133 CGCG CG^CG
AflII 1129 CTTAAG C^TTAAG
AflIII 1054 ACATGT A^C[GA][CT]GT
AhdI 1210 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1210 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 560 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 750 GGACC G^G[AT]CC
AviII 1590 TGCGCA TGC^GCA
BalI 732 TGGCCA TGG^CCA
BamHI 2007 GGATCC G^GATCC
BanII 852 GGGCTC G[GA]GC[CT]^C
BbvCI 1261 GCTGAGG CCTCAGC(-5/-2)
BcgI 709 CGAATTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1129 CTTAAG C^TTAAG
BglI 131 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1516 GCTGAGC GC^T[ACGT]AGC
BmyI 852 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 1516 GCTGAGC GC^T[ACGT]AGC
BsmFI 970 GTCCC GGGAC(10/14)
BsmI 715 GAATGC GAATGC(1/-1)
Bsp1286I 852 GGGCTC G[AGT]GC[ACT]^C
BspHI 1308 TCATGA T^CATGA
BspLU11I 1054 ACATGT A^CATGT
BspMI 1521 GCAGGT ACCTGC(4/8)
BsrFI 960 GCCGGT [GA]^CCGG[CT]
Bst1107I 201 GTATAC GTA^TAC
BstEII 75 GGTGACC G^GT[ACGT]ACC
BstPI 75 GGTGACC G^GT[ACGT]ACC
BstUI 133 CGCG CG^CG
BstXI 1871 CCAACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 201 GTATAC GTA^TAC
CelII 1516 GCTGAGC GC^T[ACGT]AGC
CfoI 1591 GCGC GCG^C
Cfr10I 960 GCCGGT [GA]^CCGG[CT]
DraI 243 TTTAAA TTT^AAA
EaeI 732 TGGCCA [CT]^GGCC[GA]
Eam1105I 1210 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 75 GGTGACC G^GT[ACGT]ACC
EcoRV 897 GATATC GAT^ATC
EcoT22I 836 ATGCAT ATGCA^T
FspI 1590 TGCGCA TGC^GCA
HapII 961 CCGG C^CGG
HhaI 1591 GCGC GCG^C
HinP1I 1591 GCGC G^CGC
HincII 1765 GTCAAC GT[CT]^[GA]AC
HindII 1765 GTCAAC GT[CT]^[GA]AC
HpaII 961 CCGG C^CGG
KspI 132 CCGCGG CCGC^GG
MluNI 732 TGGCCA TGG^CCA
MscI 732 TGGCCA TGG^CCA
MslI 69 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 961 CCGG C^CGG
MvnI 133 CGCG CG^CG
NcoI 782 CCATGG C^CATGG
NdeI 1709 CATATG CA^TATG
NsiI 836 ATGCAT ATGCA^T
PflFI 560 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1308 TCATGA T^CATGA
SacII 132 CCGCGG CCGC^GG
SapI 976 GAAGAGC GCTCTTC(1/4)
SphI 834 GCATGC GCATG^C
SspI 2162 AATATT AAT^ATT
TaqI 878 TCGA T^CGA
Tth111I 560 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 878 TCGA T^CGA
XbaI 289 TCTAGA T^CTAGA
XcmI 912 CCACTATAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Asp700I 715 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1363 GAAACATTTC
BciVI 1050 GGATAC GTATCC(6/5)
1655 GTATCC
BclI 165 TGATCA T^GATCA
948 TGATCA
BsaI 558 GAGACC GGTCTC(1/5)
1989 GAGACC
BseRI 497 CTCCTC GAGGAG(10/8)
555 GAGGAG
Bsp1407I 457 TGTACA T^GTACA
1564 TGTACA
BsrGI 457 TGTACA T^GTACA
1564 TGTACA
BstYI 756 GGATCT [GA]^GATC[CT]
2007 GGATCC
Cfr13I 60 GGGCC G^G[ACGT]CC
750 GGACC
DraIII 216 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
341 CACCCAGTG
DsaI 132 CCGCGG C^C[GA][CT]GG
782 CCATGG
EarI 976 GAAGAG CTCTTC(1/4)
1681 GAAGAG
FbaI 165 TGATCA T^GATCA
948 TGATCA
Ksp632I 976 GAAGAG CTCTTC(1/4)
1681 GAAGAG
MaeII 1098 ACGT A^CGT
2106 ACGT
MflI 756 GGATCT [GA]^GATC[CT]
2007 GGATCC
PflMI 645 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1816 CCATTTTATGG
Sau96I 60 GGGCC G^G[ACGT]CC
750 GGACC
ScaI 269 AGTACT AGT^ACT
1289 AGTACT
SfcI 410 CTACAG C^T[GA][CT]AG
1085 CTGTAG
SmlI 1129 CTTAAG C^T[CT][GA]AG
1915 CTTGAG
SspBI 457 TGTACA T^GTACA
1564 TGTACA
Van91I 645 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1816 CCATTTTATGG
XhoII 756 GGATCT [GA]^GATC[CT]
2007 GGATCC
XmnI 715 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1363 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BfaI BpmI BsaJI BsiYI
BslI BsmAI BsrDI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI