Restriction map for
pF1KB3247
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanII BbeI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HindIII KasI KpnI
Ksp632I KspI MfeI MluI MluNI
MroI MscI MunI MvnI MwoI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 794 AAATTC [GA]^AATT[CT]
AlwNI 624 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 794 AAATTC [GA]^AATT[CT]
BamHI 269 GGATCC G^GATCC
BanI 454 GGCACC G^G[CT][GA]CC
BcnI 266 CCCGG CC^[GC]GG
BfaI 901 CTAG C^TAG
BlpI 48 GCTGAGC GC^T[ACGT]AGC
BmyI 250 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
BsaBI 716 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 543 CTCCTC GAGGAG(10/8)
BsgI 290 GTGCAG GTGCAG(16/14)
Bsp1286I 250 GGGCAC G[AGT]GC[ACT]^C
BspDI 64 ATCGAT AT^CGAT
BssSI 40 CTCGTG CACGAG(-5/-1)
BstXI 684 CCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 48 GCTGAGC GC^T[ACGT]AGC
CfoI 58 GCGC GCG^C
ClaI 64 ATCGAT AT^CGAT
DraII 697 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 253 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 153 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 697 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 833 CCTTGG C^C[AT][AT]GG
FauI 458 CCCGC CCCGC(4/6)
HapII 267 CCGG C^CGG
HhaI 58 GCGC GCG^C
HinP1I 58 GCGC G^CGC
HpaI 442 GTTAAC GTT^AAC
HpaII 267 CCGG C^CGG
MaeI 901 CTAG C^TAG
MamI 716 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 443 TTAA T^TAA
MspI 267 CCGG C^CGG
NciI 266 CCCGG CC^[GC]GG
PpuMI 697 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1034 CAGCTG CAG^CTG
SbfI 227 CCTGCAGG CCTGCA^GG
Sse8387I 227 CCTGCAGG CCTGCA^GG
StyI 833 CCTTGG C^C[AT][AT]GG
Tru9I 443 TTAA T^TAA
Tsp45I 394 GTCAC ^GT[GC]AC
XcmI 702 CCATACCACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbrPI 103 CACGTG CAC^GTG
253 CACGTG
BbsI 496 GAAGAC GAAGAC(2/6)
619 GAAGAC
BclI 809 TGATCA T^GATCA
905 TGATCA
BpmI 546 CTCCAG CTGGAG(16/14)
1016 CTCCAG
BpuAI 496 GAAGAC GAAGAC(2/6)
619 GAAGAC
BsaAI 103 CACGTG [CT]AC^GT[GA]
253 CACGTG
BsaI 223 GAGACC GGTCTC(1/5)
668 GAGACC
BsrDI 858 CATTGC GCAATG(2/0)
917 GCAATG
BstYI 269 GGATCC [GA]^GATC[CT]
715 GGATCT
Cac8I 18 GCACGC GC[ACGT]^[ACGT]GC
964 GCCAGC
FbaI 809 TGATCA T^GATCA
905 TGATCA
HincII 442 GTTAAC GT[CT]^[GA]AC
865 GTCAAC
HindII 442 GTTAAC GT[CT]^[GA]AC
865 GTCAAC
MaeIII 394 GTCAC ^GT[ACGT]AC
575 GTTAC
MflI 269 GGATCC [GA]^GATC[CT]
715 GGATCT
PmaCI 103 CACGTG CAC^GTG
253 CACGTG
PmlI 103 CACGTG CAC^GTG
253 CACGTG
PstI 228 CTGCAG CTGCA^G
1054 CTGCAG
TaqI 65 TCGA T^CGA
873 TCGA
Tsp509I 426 AATT ^AATT
795 AATT
TthHB8I 65 TCGA T^CGA
873 TCGA
XhoII 269 GGATCC [GA]^GATC[CT]
715 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MboI
MboII MnlI MslI MspA1I MvaI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI TfiI TseI TspRI