Restriction map for
pF1KB3288
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BbrPI
BbsI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BseAI BsiEI BsiHKAI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI Ksp632I KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfuI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 906 GGTACC G^GTACC
AccI 1978 GTCTAC GT^[AC][GT]AC
AcyI 285 GGCGCC G[GA]^CG[CT]C
AgeI 1881 ACCGGT A^CCGGT
AocI 1441 CCTAAGG CC^T[ACGT]AGG
Asp718I 906 GGTACC G^GTACC
BalI 188 TGGCCA TGG^CCA
BbeI 285 GGCGCC GGCGC^C
BfaI 1514 CTAG C^TAG
BsaAI 2061 CACGTA [CT]AC^GT[GA]
BsaHI 285 GGCGCC G[GA]^CG[CT]C
BsaI 2132 GAGACC GGTCTC(1/5)
BsaWI 1881 ACCGGT [AT]^CCGG[AT]
BsgI 2108 GTGCAG GTGCAG(16/14)
BsiWI 2063 CGTACG C^GTACG
BsmI 491 GAATGC GAATGC(1/-1)
Bsp1407I 2057 TGTACA T^GTACA
BsrGI 2057 TGTACA T^GTACA
BstAPI 1005 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2094 CCAGGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1441 CCTAAGG CC^T[ACGT]AGG
EaeI 188 TGGCCA [CT]^GGCC[GA]
Eco81I 1441 CCTAAGG CC^T[ACGT]AGG
EcoRV 458 GATATC GAT^ATC
FauI 1941 CCCGC CCCGC(4/6)
HaeII 285 GGCGCC [GA]GCGC^[CT]
Hin1I 285 GGCGCC G[GA]^CG[CT]C
KasI 285 GGCGCC G^GCGCC
KpnI 906 GGTACC GGTAC^C
MaeI 1514 CTAG C^TAG
MboII 1955 TCTTC GAAGA(8/7)
MluNI 188 TGGCCA TGG^CCA
MscI 188 TGGCCA TGG^CCA
MslI 513 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 285 GGCGCC GG^CGCC
PinAI 1881 ACCGGT A^CCGGT
PleI 1412 GACTC GAGTC(4/5)
SexAI 1272 ACCAGGT A^CC[AT]GGT
SfoI 285 GGCGCC GGC^GCC
SgrAI 1880 CACCGGTG C[GA]^CCGG[CT]G
SphI 518 GCATGC GCATG^C
SspBI 2057 TGTACA T^GTACA
TaqI 672 TCGA T^CGA
TthHB8I 672 TCGA T^CGA
XcmI 225 CCACTTGCAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 2018 ACGTGT A^C[GA][CT]GT
2060 ACACGT
AlwNI 25 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
154 CAGCCCCTG
BamHI 572 GGATCC G^GATCC
965 GGATCC
BseRI 122 CTCCTC GAGGAG(10/8)
2199 CTCCTC
BsmAI 722 GAGAC GTCTC(1/5)
2132 GAGAC
BsrFI 282 ACCGGC [GA]^CCGG[CT]
1881 ACCGGT
BsrI 341 CCAGT ACTGG(1/-1)
1894 CCAGT
BstEII 641 GGTTACC G^GT[ACGT]ACC
1078 GGTGACC
BstPI 641 GGTTACC G^GT[ACGT]ACC
1078 GGTGACC
BstYI 572 GGATCC [GA]^GATC[CT]
965 GGATCC
CfoI 286 GCGC GCG^C
748 GCGC
Cfr10I 282 ACCGGC [GA]^CCGG[CT]
1881 ACCGGT
EcoNI 1588 CCTGGTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2088 CCTGGACCAGG
EcoO65I 641 GGTTACC G^GT[ACGT]ACC
1078 GGTGACC
HhaI 286 GCGC GCG^C
748 GCGC
HinP1I 286 GCGC G^CGC
748 GCGC
MflI 572 GGATCC [GA]^GATC[CT]
965 GGATCC
MseI 434 TTAA T^TAA
1904 TTAA
NcoI 206 CCATGG C^CATGG
512 CCATGG
NspI 179 ACATGC [GA]CATG^[CT]
518 GCATGC
PstI 22 CTGCAG CTGCA^G
2168 CTGCAG
PvuII 25 CAGCTG CAG^CTG
1712 CAGCTG
SfcI 22 CTGCAG C^T[GA][CT]AG
2168 CTGCAG
SmlI 698 CTCAAG C^T[CT][GA]AG
1655 CTCAAG
Tru9I 434 TTAA T^TAA
1904 TTAA
Tsp45I 826 GTGAC ^GT[GC]AC
1079 GTGAC
XhoII 572 GGATCC [GA]^GATC[CT]
965 GGATCC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AvaII BanI BanII
BbvI BciVI BcnI BglI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfiI StuI StyI TfiI TseI
Tsp509I TspRI Van91I