Restriction map for
pF1KB3300
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp718I
AspI AvrII BbrPI BbsI BcgI
BciVI BclI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsaI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 556 GTCTAC GT^[AC][GT]AC
AhdI 1486 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1520 AGCGCT AGC^GCT
Asp700I 750 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1486 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 815 GGCGCC GGCGC^C
BbvCI 1530 CCTCAGC CCTCAGC(-5/-2)
BfaI 256 CTAG C^TAG
BglII 258 AGATCT A^GATCT
BsgI 2008 GTGCAG GTGCAG(16/14)
BsmBI 1836 CGTCTC CGTCTC(1/5)
BssSI 1858 CACGAG CACGAG(-5/-1)
Eam1105I 1486 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1520 AGCGCT AGC^GCT
EcoT22I 1399 ATGCAT ATGCA^T
KasI 815 GGCGCC G^GCGCC
KspI 565 CCGCGG CCGC^GG
MaeI 256 CTAG C^TAG
MslI 1203 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 583 GCCGGC GCC^GGC
NarI 815 GGCGCC GG^CGCC
NcoI 551 CCATGG C^CATGG
NgoMIV 583 GCCGGC G^CCGGC
NsiI 1399 ATGCAT ATGCA^T
NspI 151 ACATGC [GA]CATG^[CT]
PaeR7I 802 CTCGAG C^TCGAG
PflMI 1716 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1817 GGGACCT [GA]G^G[AT]CC[CT]
SacII 565 CCGCGG CCGC^GG
SbfI 1338 CCTGCAGG CCTGCA^GG
ScaI 650 AGTACT AGT^ACT
SfoI 815 GGCGCC GGC^GCC
Sse8387I 1338 CCTGCAGG CCTGCA^GG
Van91I 1716 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 255 TCTAGA T^CTAGA
XhoI 802 CTCGAG C^TCGAG
XmnI 750 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 566 CGCG CG^CG
1350 CGCG
AccIII 14 TCCGGA T^CCGGA
1868 TCCGGA
ApaI 709 GGGCCC GGGCC^C
1526 GGGCCC
AvaI 692 CTCGGG C^[CT]CG[GA]G
802 CTCGAG
AviII 1770 TGCGCA TGC^GCA
2108 TGCGCA
BalI 1823 TGGCCA TGG^CCA
2070 TGGCCA
BamHI 1013 GGATCC G^GATCC
1901 GGATCC
BanI 580 GGTGCC G^G[CT][GA]CC
815 GGCGCC
BcnI 911 CCCGG CC^[GC]GG
1587 CCGGG
BsaWI 14 TCCGGA [AT]^CCGG[AT]
1868 TCCGGA
BseAI 14 TCCGGA T^CCGGA
1868 TCCGGA
BsoBI 692 CTCGGG C^[CT]CG[GA]G
802 CTCGAG
BspEI 14 TCCGGA T^CCGGA
1868 TCCGGA
BsrFI 583 GCCGGC [GA]^CCGG[CT]
1196 ACCGGC
BstAPI 1167 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1752 GCACTACCTGC
BstBI 446 TTCGAA TT^CGAA
515 TTCGAA
BstUI 566 CGCG CG^CG
1350 CGCG
Cfr10I 583 GCCGGC [GA]^CCGG[CT]
1196 ACCGGC
EcoNI 1728 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1821 CCTGGCCAAGG
EcoT14I 551 CCATGG C^C[AT][AT]GG
1826 CCAAGG
FspI 1770 TGCGCA TGC^GCA
2108 TGCGCA
MluNI 1823 TGGCCA TGG^CCA
2070 TGGCCA
MroI 14 TCCGGA T^CCGGA
1868 TCCGGA
MscI 1823 TGGCCA TGG^CCA
2070 TGGCCA
MvnI 566 CGCG CG^CG
1350 CGCG
NciI 911 CCCGG CC^[GC]GG
1587 CCGGG
NspV 446 TTCGAA TT^CGAA
515 TTCGAA
SacI 118 GAGCTC GAGCT^C
1582 GAGCTC
SapI 1579 GAAGAGC GCTCTTC(1/4)
6 GCTCTTC
SfuI 446 TTCGAA TT^CGAA
515 TTCGAA
SmlI 726 CTCAAG C^T[CT][GA]AG
802 CTCGAG
StuI 1247 AGGCCT AGG^CCT
2012 AGGCCT
StyI 551 CCATGG C^C[AT][AT]GG
1826 CCAAGG
Tsp509I 663 AATT ^AATT
1636 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I XhoII