Restriction map for
pF1KB3423
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BfrI
BglII BlnI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsiEI BsiHKAI
BsiWI BsmAI BsmBI Bsp1286I Bsp1407I
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluI MluNI MscI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 89 TCCGGA T^CCGGA
Asp700I 105 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 71 CTCGGG C^[CT]CG[GA]G
BbvCI 547 CCTCAGC CCTCAGC(-5/-2)
BcnI 717 CCCGG CC^[GC]GG
BfaI 29 CTAG C^TAG
BglI 634 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 89 TCCGGA [AT]^CCGG[AT]
BseAI 89 TCCGGA T^CCGGA
BseRI 388 GAGGAG GAGGAG(10/8)
BsmFI 792 GGGAC GGGAC(10/14)
BsmI 729 GAATGC GAATGC(1/-1)
BsoBI 71 CTCGGG C^[CT]CG[GA]G
BspDI 343 ATCGAT AT^CGAT
BspEI 89 TCCGGA T^CCGGA
BstAPI 669 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 990 GGTGACC G^GT[ACGT]ACC
BstPI 990 GGTGACC G^GT[ACGT]ACC
ClaI 343 ATCGAT AT^CGAT
DsaI 305 CCATGG C^C[GA][CT]GG
EaeI 735 CGGCCA [CT]^GGCC[GA]
EarI 242 CTCTTC CTCTTC(1/4)
EcoNI 766 CCTGTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 990 GGTGACC G^GT[ACGT]ACC
EcoRV 298 GATATC GAT^ATC
FauI 919 GCGGG CCCGC(4/6)
HgaI 381 GCGTC GACGC(5/10)
HincII 237 GTTGAC GT[CT]^[GA]AC
HindII 237 GTTGAC GT[CT]^[GA]AC
Ksp632I 242 CTCTTC CTCTTC(1/4)
MaeI 29 CTAG C^TAG
MroI 89 TCCGGA T^CCGGA
NaeI 733 GCCGGC GCC^GGC
NciI 717 CCCGG CC^[GC]GG
NcoI 305 CCATGG C^CATGG
NgoMIV 733 GCCGGC G^CCGGC
NlaIV 981 GGCCCC GG[ACGT]^[ACGT]CC
PpuMI 926 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 837 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 742 CTGCAG CTGCA^G
ScaI 428 AGTACT AGT^ACT
SmlI 779 CTCAAG C^T[CT][GA]AG
TfiI 255 GATTC G^A[AT]TC
XmnI 105 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 93 GAATTT [GA]^AATT[CT]
319 AAATTT
ApoI 93 GAATTT [GA]^AATT[CT]
319 AAATTT
AvaII 843 GGTCC G^G[AT]CC
927 GGACC
BclI 179 TGATCA T^GATCA
324 TGATCA
BsaBI 222 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
531 GATCATCATC
BsrFI 733 GCCGGC [GA]^CCGG[CT]
994 ACCGGC
Cfr10I 733 GCCGGC [GA]^CCGG[CT]
994 ACCGGC
DraII 926 AGGACCT [GA]G^G[ACGT]CC[CT]
980 AGGCCCC
EcoO109I 926 AGGACCT [GA]G^G[ACGT]CC[CT]
980 AGGCCCC
EcoT14I 209 CCAAGG C^C[AT][AT]GG
305 CCATGG
FbaI 179 TGATCA T^GATCA
324 TGATCA
HaeII 192 AGCGCC [GA]GCGC^[CT]
275 AGCGCC
MamI 222 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
531 GATCATCATC
MseI 97 TTAA T^TAA
143 TTAA
MslI 564 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
666 CATGCAGGTG
PleI 240 GACTC GAGTC(4/5)
282 GACTC
PvuII 724 CAGCTG CAG^CTG
739 CAGCTG
SfaNI 40 GATGC GCATC(5/9)
675 GATGC
StyI 209 CCAAGG C^C[AT][AT]GG
305 CCATGG
TaqI 246 TCGA T^CGA
344 TCGA
Tru9I 97 TTAA T^TAA
143 TTAA
TthHB8I 246 TCGA T^CGA
344 TCGA
XcmI 645 CCAGAGTGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
870 CCAGTTCACAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BlpI Bpu1102I BsaJI BsgI BsiYI
BslI BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfcI
TseI Tsp45I Tsp509I TspRI XhoII