################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BbeI BbrPI BciVI BcnI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KspI MamI MluI MroI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 2015 GGTACC G^GTACC AflIII 1772 ACATGT A^C[GA][CT]GT Asp718I 2015 GGTACC G^GTACC BglI 793 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1448 CTGGAG CTGGAG(16/14) BsaI 3116 GAGACC GGTCTC(1/5) BsiEI 205 CGGTCG CG[GA][CT]^CG BsmFI 3201 GTCCC GGGAC(10/14) BsmI 181 GAATGC GAATGC(1/-1) BspLU11I 1772 ACATGT A^CATGT BssSI 3172 CACGAG CACGAG(-5/-1) BstBI 2402 TTCGAA TT^CGAA DraII 1610 AGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 2296 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2690 CCACGG C^C[GA][CT]GG EcoO109I 1610 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 1871 CCAAGG C^C[AT][AT]GG HindIII 1424 AAGCTT A^AGCTT KpnI 2015 GGTACC GGTAC^C NdeI 2465 CATATG CA^TATG NspV 2402 TTCGAA TT^CGAA PshAI 1570 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2674 GAGCTC GAGCT^C SalI 2916 GTCGAC G^TCGAC SfuI 2402 TTCGAA TT^CGAA SpeI 2575 ACTAGT A^CTAGT StyI 1871 CCAAGG C^C[AT][AT]GG XbaI 3429 TCTAGA T^CTAGA XcmI 2690 CCACGGCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccI 715 GTAGAC GT^[AC][GT]AC 2916 GTCGAC AlwNI 3007 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG 3187 CAGCAGCTG AspHI 2674 GAGCTC G[AT]GC[AT]^C 3230 GAGCAC BalI 2 TGGCCA TGG^CCA 2346 TGGCCA BamHI 225 GGATCC G^GATCC 2354 GGATCC BanI 2015 GGTACC G^G[CT][GA]CC 2155 GGCACC BanII 598 GAGCCC G[GA]GC[CT]^C 2674 GAGCTC BbvCI 3126 CCTCAGC CCTCAGC(-5/-2) 3097 GCTGAGG BclI 1703 TGATCA T^GATCA 3080 TGATCA BsaAI 2161 TACGTA [CT]AC^GT[GA] 2201 TACGTA BsiHKAI 2674 GAGCTC G[AT]GC[AT]^C 3230 GAGCAC Bsp1407I 1034 TGTACA T^GTACA 1138 TGTACA BspHI 2036 TCATGA T^CATGA 2922 TCATGA BspMI 3119 ACCTGC ACCTGC(4/8) 2115 GCAGGT BsrGI 1034 TGTACA T^GTACA 1138 TGTACA BstXI 230 CCAAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3389 CCAAAGAAATGG DraI 2793 TTTAAA TTT^AAA 2823 TTTAAA EaeI 2 TGGCCA [CT]^GGCC[GA] 2346 TGGCCA EcoNI 1149 CCTTTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2934 CCTAGAGAAGG FbaI 1703 TGATCA T^GATCA 3080 TGATCA HapII 2293 CCGG C^CGG 2381 CCGG HgaI 3009 GACGC GACGC(5/10) 1601 GCGTC HincII 2881 GTTGAC GT[CT]^[GA]AC 2916 GTCGAC HindII 2881 GTTGAC GT[CT]^[GA]AC 2916 GTCGAC HpaII 2293 CCGG C^CGG 2381 CCGG MfeI 1823 CAATTG C^AATTG 1975 CAATTG MluNI 2 TGGCCA TGG^CCA 2346 TGGCCA MscI 2 TGGCCA TGG^CCA 2346 TGGCCA MslI 813 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2223 CATGGTTATG MspA1I 1417 CAGCTG C[AC]G^C[GT]G 3190 CAGCTG MspI 2293 CCGG C^CGG 2381 CCGG MunI 1823 CAATTG C^AATTG 1975 CAATTG PflMI 936 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 948 CCATCCTCTGG PstI 300 CTGCAG CTGCA^G 1233 CTGCAG PvuII 1417 CAGCTG CAG^CTG 3190 CAGCTG RcaI 2036 TCATGA T^CATGA 2922 TCATGA SnaBI 2161 TACGTA TAC^GTA 2201 TACGTA SspBI 1034 TGTACA T^GTACA 1138 TGTACA StuI 381 AGGCCT AGG^CCT 852 AGGCCT Van91I 936 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 948 CCATCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbsI BbvI BcgI BfaI BglII BmyI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRI EcoRII EcoRV FauI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII