Restriction map for
pF1KB3567
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI MamI MfeI MluI MunI
NarI NcoI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 189 GGTACC G^GTACC
AcyI 1066 GACGCC G[GA]^CG[CT]C
AhdI 1234 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 532 GTGCAC G^TGCAC
AlwNI 196 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 532 GTGCAC G^TGCAC
Asp718I 189 GGTACC G^GTACC
AspEI 1234 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 481 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 680 TGCGCA TGC^GCA
BglI 1295 GCCGCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1066 GACGCC G[GA]^CG[CT]C
BsaI 2047 GAGACC GGTCTC(1/5)
BsmI 96 GAATGC GAATGC(1/-1)
BsrBI 727 GAGCGG CCGCTC(-3/-3)
BssHII 1414 GCGCGC G^CGCGC
BstEII 660 GGTTACC G^GT[ACGT]ACC
BstPI 660 GGTTACC G^GT[ACGT]ACC
BstXI 68 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 394 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1999 GACCTGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1234 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 660 GGTTACC G^GT[ACGT]ACC
FspI 680 TGCGCA TGC^GCA
Hin1I 1066 GACGCC G[GA]^CG[CT]C
HincII 2008 GTCGAC GT[CT]^[GA]AC
HindII 2008 GTCGAC GT[CT]^[GA]AC
KpnI 189 GGTACC GGTAC^C
KspI 1139 CCGCGG CCGC^GG
MseI 2114 TTAA T^TAA
NdeI 956 CATATG CA^TATG
PflFI 481 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 214 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1969 GAGCTC GAGCT^C
SacII 1139 CCGCGG CCGC^GG
SalI 2008 GTCGAC G^TCGAC
SgrAI 20 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1471 CCCGGG CCC^GGG
Tru9I 2114 TTAA T^TAA
Tth111I 481 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 771 TCTAGA T^CTAGA
XmaCI 1471 CCCGGG C^CCGGG
XmaI 1471 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1333 GTCTAC GT^[AC][GT]AC
2008 GTCGAC
AvaI 1409 CTCGGG C^[CT]CG[GA]G
1471 CCCGGG
BalI 1397 TGGCCA TGG^CCA
1925 TGGCCA
BglII 1046 AGATCT A^GATCT
2082 AGATCT
BsmBI 1406 CGTCTC CGTCTC(1/5)
1207 GAGACG
BsoBI 1409 CTCGGG C^[CT]CG[GA]G
1471 CCCGGG
BsrDI 360 CATTGC GCAATG(2/0)
1802 GCAATG
DsaI 263 CCACGG C^C[GA][CT]GG
1139 CCGCGG
EagI 1302 CGGCCG C^GGCCG
1367 CGGCCG
EclXI 1302 CGGCCG C^GGCCG
1367 CGGCCG
Eco52I 1302 CGGCCG C^GGCCG
1367 CGGCCG
EcoNI 111 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1437 CCTGCGAGAGG
EcoT14I 587 CCAAGG C^C[AT][AT]GG
1745 CCAAGG
MaeII 482 ACGT A^CGT
947 ACGT
MluNI 1397 TGGCCA TGG^CCA
1925 TGGCCA
MscI 1397 TGGCCA TGG^CCA
1925 TGGCCA
NaeI 436 GCCGGC GCC^GGC
1365 GCCGGC
NgoMIV 436 GCCGGC G^CCGGC
1365 GCCGGC
PflMI 195 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1166 CCACCTTCTGG
PstI 148 CTGCAG CTGCA^G
1248 CTGCAG
SfcI 148 CTGCAG C^T[GA][CT]AG
1248 CTGCAG
StuI 176 AGGCCT AGG^CCT
578 AGGCCT
StyI 587 CCAAGG C^C[AT][AT]GG
1745 CCAAGG
TaqI 1502 TCGA T^CGA
2009 TCGA
TthHB8I 1502 TCGA T^CGA
2009 TCGA
Van91I 195 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1166 CCACCTTCTGG
XcmI 101 CCATCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1162 CCAGCCACCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspEI BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MroI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TseI Tsp45I Tsp509I
TspRI XhoII