Restriction map for
pF1KB3698
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BciVI
BclI BfaI BfrI BglII BlnI
BsaHI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BsrDI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MfeI MflI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI StyI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoII
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 1029 AAATTT [GA]^AATT[CT]
AflIII 419 ACATGT A^C[GA][CT]GT
ApoI 1029 AAATTT [GA]^AATT[CT]
AvaII 312 GGACC G^G[AT]CC
BbvCI 1002 GCTGAGG CCTCAGC(-5/-2)
BcgI 776 GCATGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 525 GCTGAGC GC^T[ACGT]AGC
BpmI 34 CTGGAG CTGGAG(16/14)
Bpu1102I 525 GCTGAGC GC^T[ACGT]AGC
BsaAI 163 TACGTG [CT]AC^GT[GA]
BsaBI 270 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 985 GAGACC GGTCTC(1/5)
BsiEI 459 CGACCG CG[GA][CT]^CG
BsmAI 985 GAGAC GTCTC(1/5)
BspLU11I 419 ACATGT A^CATGT
BstAPI 843 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 525 GCTGAGC GC^T[ACGT]AGC
DrdI 373 GACATCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 741 CCGTGG C^C[GA][CT]GG
EcoNI 336 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 105 CCCGC CCCGC(4/6)
HaeII 768 GGCGCT [GA]GCGC^[CT]
HinfI 331 GACTC G^A[ACGT]TC
MamI 270 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1035 GCCGGC GCC^GGC
NgoMIV 1035 GCCGGC G^CCGGC
NspI 419 ACATGT [GA]CATG^[CT]
PaeR7I 649 CTCGAG C^TCGAG
PleI 331 GACTC GAGTC(4/5)
PvuII 948 CAGCTG CAG^CTG
SapI 402 GCTCTTC GCTCTTC(1/4)
SbfI 408 CCTGCAGG CCTGCA^GG
SfaNI 550 GCATC GCATC(5/9)
SmaI 20 CCCGGG CCC^GGG
SmlI 649 CTCGAG C^T[CT][GA]AG
Sse8387I 408 CCTGCAGG CCTGCA^GG
StuI 608 AGGCCT AGG^CCT
Tsp509I 1030 AATT ^AATT
XcmI 212 CCATCGGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 649 CTCGAG C^TCGAG
XmaCI 20 CCCGGG C^CCGGG
XmaI 20 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 237 GATCC GGATC(4/5)
780 GATCC
Asp700I 825 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1044 GAACCGCTTC
BalI 125 TGGCCA TGG^CCA
476 TGGCCA
BanII 88 GAGCTC G[GA]GC[CT]^C
142 GAGCTC
BglI 753 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1063 GCCCTGAAGGC
BsgI 355 CTGCAC GTGCAG(16/14)
640 GTGCAG
Bsp1407I 422 TGTACA T^GTACA
1010 TGTACA
BsrFI 734 ACCGGC [GA]^CCGG[CT]
1035 GCCGGC
BsrGI 422 TGTACA T^GTACA
1010 TGTACA
BssSI 358 CACGAG CACGAG(-5/-1)
183 CTCGTG
Cfr10I 734 ACCGGC [GA]^CCGG[CT]
1035 GCCGGC
DdeI 526 CTGAG C^T[ACGT]AG
1003 CTGAG
EarI 205 CTCTTC CTCTTC(1/4)
403 CTCTTC
HgaI 858 GACGC GACGC(5/10)
431 GCGTC
HincII 415 GTCAAC GT[CT]^[GA]AC
925 GTCAAC
HindII 415 GTCAAC GT[CT]^[GA]AC
925 GTCAAC
Ksp632I 205 CTCTTC CTCTTC(1/4)
403 CTCTTC
MaeII 164 ACGT A^CGT
827 ACGT
MluNI 125 TGGCCA TGG^CCA
476 TGGCCA
MscI 125 TGGCCA TGG^CCA
476 TGGCCA
MspA1I 853 CAGCGG C[AC]G^C[GT]G
948 CAGCTG
SacI 88 GAGCTC GAGCT^C
142 GAGCTC
SspBI 422 TGTACA T^GTACA
1010 TGTACA
TaqI 458 TCGA T^CGA
650 TCGA
TspRI 643 CAGTG CA[GC]TG[ACGT][ACGT]^
1116 CAGTG
TthHB8I 458 TCGA T^CGA
650 TCGA
XmnI 825 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1044 GAACCGCTTC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
ApaLI AspHI AvaI BanI BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TseI Tsp45I