Restriction map for
pF1KB3783
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# NO CUT ENZYMES ( 100 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflIII AgeI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BbvCI BciVI
BclI BfaI BglI BlnI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HpaI
KpnI MaeI MfeI MluI MluNI
MroI MscI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflII 352 CTTAAG C^TTAAG
ApaI 94 GGGCCC GGGCC^C
BanI 753 GGCGCC G^G[CT][GA]CC
BbeI 753 GGCGCC GGCGC^C
BbrPI 2035 CACGTG CAC^GTG
BbsI 106 GAAGAC GAAGAC(2/6)
BcgI 2063 CGATGCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 352 CTTAAG C^TTAAG
BpuAI 106 GAAGAC GAAGAC(2/6)
BsaAI 2035 CACGTG [CT]AC^GT[GA]
BsaWI 1055 ACCGGA [AT]^CCGG[AT]
BsiEI 740 CGATCG CG[GA][CT]^CG
BspHI 1778 TCATGA T^CATGA
BssHII 1758 GCGCGC G^CGCGC
BstAPI 1242 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2140 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1995 GATATC GAT^ATC
HincII 2092 GTCAAC GT[CT]^[GA]AC
HindII 2092 GTCAAC GT[CT]^[GA]AC
HindIII 442 AAGCTT A^AGCTT
KasI 753 GGCGCC G^GCGCC
KspI 2200 CCGCGG CCGC^GG
MslI 1335 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 753 GGCGCC GG^CGCC
PmaCI 2035 CACGTG CAC^GTG
PmlI 2035 CACGTG CAC^GTG
PshAI 2255 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 113 CTGCAG CTGCA^G
PvuI 740 CGATCG CGAT^CG
RcaI 1778 TCATGA T^CATGA
SacII 2200 CCGCGG CCGC^GG
SfoI 753 GGCGCC GGC^GCC
StuI 1096 AGGCCT AGG^CCT
XcmI 1302 CCACTTCACCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AatII 1606 GACGTC GACGT^C
2014 GACGTC
AhdI 796 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2014 GACGTCGTGTC
Alw44I 1614 GTGCAC G^TGCAC
2137 GTGCAC
AlwNI 13 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2050 CAGCGCCTG
ApaLI 1614 GTGCAC G^TGCAC
2137 GTGCAC
AspEI 796 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2014 GACGTCGTGTC
BcnI 518 CCCGG CC^[GC]GG
1812 CCCGG
BglII 624 AGATCT A^GATCT
1245 AGATCT
BpmI 1857 CTGGAG CTGGAG(16/14)
2206 CTGGAG
BsaBI 195 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1443 GATTGAGATC
BsgI 1249 CTGCAC GTGCAG(16/14)
1276 GTGCAG
Bsp1407I 1691 TGTACA T^GTACA
1877 TGTACA
BsrFI 770 ACCGGC [GA]^CCGG[CT]
974 ACCGGC
BsrGI 1691 TGTACA T^GTACA
1877 TGTACA
BstXI 1625 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1799 CCAATTTCGTGG
Cfr10I 770 ACCGGC [GA]^CCGG[CT]
974 ACCGGC
Eam1105I 796 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2014 GACGTCGTGTC
MamI 195 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1443 GATTGAGATC
NciI 518 CCCGG CC^[GC]GG
1812 CCCGG
NcoI 875 CCATGG C^CATGG
1631 CCATGG
PflMI 600 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1034 CCATAGACTGG
PvuII 137 CAGCTG CAG^CTG
1753 CAGCTG
SacI 947 GAGCTC GAGCT^C
1552 GAGCTC
SexAI 1523 ACCTGGT A^CC[AT]GGT
2141 ACCTGGT
SfcI 113 CTGCAG C^T[GA][CT]AG
618 CTACAG
SspBI 1691 TGTACA T^GTACA
1877 TGTACA
Tsp509I 1008 AATT ^AATT
1801 AATT
Van91I 600 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1034 CCATAGACTGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI AspHI AvaI AvaII
BamHI BanII BbvI BlpI BmyI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII