################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 1239 GTCTAC GT^[AC][GT]AC AciI 1149 CCGC CCGC(-3/-1) AlwNI 67 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 447 ATTAAT AT^TAAT AsnI 447 ATTAAT AT^TAAT AvaI 1316 CTCGGG C^[CT]CG[GA]G BanI 1583 GGCACC G^G[CT][GA]CC BanII 842 GGGCTC G[GA]GC[CT]^C BciVI 552 GGATAC GTATCC(6/5) BpmI 243 CTGGAG CTGGAG(16/14) BsgI 1084 CTGCAC GTGCAG(16/14) BsmAI 412 GTCTC GTCTC(1/5) BsoBI 1316 CTCGGG C^[CT]CG[GA]G BsrDI 8 CATTGC GCAATG(2/0) BsrI 19 CCAGT ACTGG(1/-1) BstXI 235 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 300 GCAAGC GC[ACGT]^[ACGT]GC DraI 1401 TTTAAA TTT^AAA DraII 1111 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 196 CCGTGG C^C[GA][CT]GG EcoO109I 1111 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 1148 CCCGC CCCGC(4/6) HincII 224 GTCAAC GT[CT]^[GA]AC HindII 224 GTCAAC GT[CT]^[GA]AC HindIII 1195 AAGCTT A^AGCTT MaeII 164 ACGT A^CGT MspA1I 70 CAGCTG C[AC]G^C[GT]G PshBI 447 ATTAAT AT^TAAT PvuII 70 CAGCTG CAG^CTG SspI 577 AATATT AAT^ATT SwaI 1400 ATTTAAAT ATTT^AAAT Tsp45I 1166 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaII 75 GGACC G^G[AT]CC 715 GGACC BbsI 283 GAAGAC GAAGAC(2/6) 1891 GTCTTC BfaI 130 CTAG C^TAG 473 CTAG BmyI 842 GGGCTC G[AGT]GC[ACT]^C 1582 GGGCAC BpuAI 283 GAAGAC GAAGAC(2/6) 1891 GTCTTC BsaJI 53 CCCAGG C^C[ACGT][ACGT]GG 196 CCGTGG BsmI 625 GAATGC GAATGC(1/-1) 784 GAATGC Bsp1286I 842 GGGCTC G[AGT]GC[ACT]^C 1582 GGGCAC Bsp1407I 915 TGTACA T^GTACA 2001 TGTACA BsrGI 915 TGTACA T^GTACA 2001 TGTACA BssKI 54 CCAGG ^CC[ACGT]GG 242 CCTGG BstNI 54 CCAGG CC^[AT]GG 242 CCTGG EcoRI 1844 GAATTC G^AATTC 2096 GAATTC EcoRII 54 CCAGG ^CC[AT]GG 242 CCTGG EcoT22I 627 ATGCAT ATGCA^T 746 ATGCAT HaeIII 17 GGCC GG^CC 1112 GGCC MaeI 130 CTAG C^TAG 473 CTAG MslI 403 CATAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 726 CATACAGATG MvaI 54 CCAGG CC^[AT]GG 242 CCTGG NsiI 627 ATGCAT ATGCA^T 746 ATGCAT ScrFI 54 CCAGG CC^[ACGT]GG 242 CCTGG SspBI 915 TGTACA T^GTACA 2001 TGTACA TaqI 1577 TCGA T^CGA 1842 TCGA TthHB8I 1577 TCGA T^CGA 1842 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I BbvI BseRI BsiYI BslI BstF5I Cfr13I DdeI DpnII EarI Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I SfcI TfiI Tru9I TseI Tsp509I TspRI XmnI