##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
StyI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1239 GTCTAC GT^[AC][GT]AC
AciI 1149 CCGC CCGC(-3/-1)
AlwNI 67 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 447 ATTAAT AT^TAAT
AsnI 447 ATTAAT AT^TAAT
AvaI 1316 CTCGGG C^[CT]CG[GA]G
BanI 1583 GGCACC G^G[CT][GA]CC
BanII 842 GGGCTC G[GA]GC[CT]^C
BciVI 552 GGATAC GTATCC(6/5)
BpmI 243 CTGGAG CTGGAG(16/14)
BsgI 1084 CTGCAC GTGCAG(16/14)
BsmAI 412 GTCTC GTCTC(1/5)
BsoBI 1316 CTCGGG C^[CT]CG[GA]G
BsrDI 8 CATTGC GCAATG(2/0)
BsrI 19 CCAGT ACTGG(1/-1)
BstXI 235 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 300 GCAAGC GC[ACGT]^[ACGT]GC
DraI 1401 TTTAAA TTT^AAA
DraII 1111 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 196 CCGTGG C^C[GA][CT]GG
EcoO109I 1111 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 1148 CCCGC CCCGC(4/6)
HincII 224 GTCAAC GT[CT]^[GA]AC
HindII 224 GTCAAC GT[CT]^[GA]AC
HindIII 1195 AAGCTT A^AGCTT
MaeII 164 ACGT A^CGT
MspA1I 70 CAGCTG C[AC]G^C[GT]G
PshBI 447 ATTAAT AT^TAAT
PvuII 70 CAGCTG CAG^CTG
SspI 577 AATATT AAT^ATT
SwaI 1400 ATTTAAAT ATTT^AAAT
Tsp45I 1166 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 75 GGACC G^G[AT]CC
715 GGACC
BbsI 283 GAAGAC GAAGAC(2/6)
1891 GTCTTC
BfaI 130 CTAG C^TAG
473 CTAG
BmyI 842 GGGCTC G[AGT]GC[ACT]^C
1582 GGGCAC
BpuAI 283 GAAGAC GAAGAC(2/6)
1891 GTCTTC
BsaJI 53 CCCAGG C^C[ACGT][ACGT]GG
196 CCGTGG
BsmI 625 GAATGC GAATGC(1/-1)
784 GAATGC
Bsp1286I 842 GGGCTC G[AGT]GC[ACT]^C
1582 GGGCAC
Bsp1407I 915 TGTACA T^GTACA
2001 TGTACA
BsrGI 915 TGTACA T^GTACA
2001 TGTACA
BssKI 54 CCAGG ^CC[ACGT]GG
242 CCTGG
BstNI 54 CCAGG CC^[AT]GG
242 CCTGG
EcoRI 1844 GAATTC G^AATTC
2096 GAATTC
EcoRII 54 CCAGG ^CC[AT]GG
242 CCTGG
EcoT22I 627 ATGCAT ATGCA^T
746 ATGCAT
HaeIII 17 GGCC GG^CC
1112 GGCC
MaeI 130 CTAG C^TAG
473 CTAG
MslI 403 CATAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
726 CATACAGATG
MvaI 54 CCAGG CC^[AT]GG
242 CCTGG
NsiI 627 ATGCAT ATGCA^T
746 ATGCAT
ScrFI 54 CCAGG CC^[ACGT]GG
242 CCTGG
SspBI 915 TGTACA T^GTACA
2001 TGTACA
TaqI 1577 TCGA T^CGA
1842 TCGA
TthHB8I 1577 TCGA T^CGA
1842 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbvI BseRI BsiYI BslI BstF5I
Cfr13I DdeI DpnII EarI Fnu4HI
FokI HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I SfcI TfiI Tru9I TseI
Tsp509I TspRI XmnI