Restriction map for
pF1KB3951
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanII BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FokI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfiI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 38 CGCG CG^CG
AcyI 3 GGCGCC G[GA]^CG[CT]C
AflIII 876 ACGTGT A^C[GA][CT]GT
Alw44I 718 GTGCAC G^TGCAC
AlwNI 590 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 718 GTGCAC G^TGCAC
AvaI 626 CCCGAG C^[CT]CG[GA]G
BanI 3 GGCGCC G^G[CT][GA]CC
BbeI 3 GGCGCC GGCGC^C
BbsI 817 GTCTTC GAAGAC(2/6)
BbvI 454 GCAGC GCAGC(8/12)
BciVI 606 GGATAC GTATCC(6/5)
BcnI 855 CCGGG CC^[GC]GG
BpuAI 817 GTCTTC GAAGAC(2/6)
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsaI 271 GAGACC GGTCTC(1/5)
BsmAI 271 GAGAC GTCTC(1/5)
BsmFI 623 GTCCC GGGAC(10/14)
BsoBI 626 CCCGAG C^[CT]CG[GA]G
BspHI 339 TCATGA T^CATGA
BstBI 527 TTCGAA TT^CGAA
BstUI 38 CGCG CG^CG
BstYI 591 AGATCC [GA]^GATC[CT]
DdeI 105 CTTAG C^T[ACGT]AG
DrdI 759 GACAGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 109 GAAGAG CTCTTC(1/4)
EcoNI 556 CCTCATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 3 GGCGCC [GA]GCGC^[CT]
HaeIII 308 GGCC GG^CC
HapII 855 CCGG C^CGG
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HpaII 855 CCGG C^CGG
KasI 3 GGCGCC G^GCGCC
Ksp632I 109 GAAGAG CTCTTC(1/4)
MflI 591 AGATCC [GA]^GATC[CT]
MslI 228 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 855 CCGG C^CGG
MvnI 38 CGCG CG^CG
MwoI 292 GCATTAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 3 GGCGCC GG^CGCC
NciI 855 CCGGG CC^[GC]GG
NspI 440 ACATGC [GA]CATG^[CT]
NspV 527 TTCGAA TT^CGAA
PleI 112 GAGTC GAGTC(4/5)
PstI 699 CTGCAG CTGCA^G
RcaI 339 TCATGA T^CATGA
SfoI 3 GGCGCC GGC^GCC
SfuI 527 TTCGAA TT^CGAA
SpeI 714 ACTAGT A^CTAGT
SspI 799 AATATT AAT^ATT
TseI 454 GCAGC G^C[AT]GC
XcmI 387 CCAAACCAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 591 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 247 GTAGAC GT^[AC][GT]AC
347 GTAGAC
AfaI 51 GTAC GT^AC
425 GTAC
AluI 456 AGCT AG^CT
752 AGCT
AlwI 592 GATCC GGATC(4/5)
519 GGATC
AspHI 704 GAGCAC G[AT]GC[AT]^C
718 GTGCAC
BfaI 10 CTAG C^TAG
715 CTAG
BmyI 704 GAGCAC G[AGT]GC[ACT]^C
718 GTGCAC
BpmI 200 CTGGAG CTGGAG(16/14)
414 CTGGAG
BseRI 100 CTCCTC GAGGAG(10/8)
629 GAGGAG
BsiHKAI 704 GAGCAC G[AT]GC[AT]^C
718 GTGCAC
BsiYI 533 CCATCGAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
556 CCTCATAAAGG
BslI 533 CCATCGAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
556 CCTCATAAAGG
Bsp1286I 704 GAGCAC G[AGT]GC[ACT]^C
718 GTGCAC
BspMI 53 ACCTGC ACCTGC(4/8)
634 GCAGGT
CfoI 4 GCGC GCG^C
37 GCGC
DsaI 162 CCATGG C^C[GA][CT]GG
486 CCATGG
EcoRV 157 GATATC GAT^ATC
505 GATATC
EcoT14I 162 CCATGG C^C[AT][AT]GG
486 CCATGG
HhaI 4 GCGC GCG^C
37 GCGC
HinP1I 4 GCGC G^CGC
37 GCGC
HphI 238 GGTGA GGTGA(8/7)
756 GGTGA
MaeI 10 CTAG C^TAG
715 CTAG
MaeII 89 ACGT A^CGT
876 ACGT
NcoI 162 CCATGG C^CATGG
486 CCATGG
NlaIV 3 GGCGCC GG[ACGT]^[ACGT]CC
210 GGTTCC
RsaI 51 GTAC GT^AC
425 GTAC
SfcI 261 CTATAG C^T[GA][CT]AG
699 CTGCAG
StyI 162 CCATGG C^C[AT][AT]GG
486 CCATGG
TfiI 543 GAATC G^A[AT]TC
868 GATTC
TspRI 146 CAGTG CA[GC]TG[ACGT][ACGT]^
515 CACTG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AcsI ApoI BsaJI BsrI
BssKI BstNI DpnII EcoRII Fnu4HI
HinfI ItaI MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
Sau3AI ScrFI TaqI Tru9I Tsp45I
Tsp509I TthHB8I