Restriction map for
pF1KB3956
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbeI
BbvCI BcgI BciVI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FseI HaeII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 1055 GGCGTC G[GA]^CG[CT]C
AlwNI 761 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 699 GGGCCC GGGCC^C
AvaI 740 CCCGGG C^[CT]CG[GA]G
BbrPI 373 CACGTG CAC^GTG
BbsI 813 GAAGAC GAAGAC(2/6)
BclI 980 TGATCA T^GATCA
BpuAI 813 GAAGAC GAAGAC(2/6)
BsaHI 1055 GGCGTC G[GA]^CG[CT]C
BseRI 63 CTCCTC GAGGAG(10/8)
BsgI 1048 CTGCAC GTGCAG(16/14)
BsmI 836 GCATTC GAATGC(1/-1)
BsoBI 740 CCCGGG C^[CT]CG[GA]G
BspMI 398 ACCTGC ACCTGC(4/8)
BstEII 708 GGTCACC G^GT[ACGT]ACC
BstPI 708 GGTCACC G^GT[ACGT]ACC
DdeI 1099 CTCAG C^T[ACGT]AG
DraII 699 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 987 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 487 CCACGG C^C[GA][CT]GG
EcoO109I 699 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 708 GGTCACC G^GT[ACGT]ACC
EcoRV 458 GATATC GAT^ATC
EcoT14I 524 CCTTGG C^C[AT][AT]GG
FbaI 980 TGATCA T^GATCA
HgaI 1056 GCGTC GACGC(5/10)
Hin1I 1055 GGCGTC G[GA]^CG[CT]C
HincII 391 GTCAAC GT[CT]^[GA]AC
HindII 391 GTCAAC GT[CT]^[GA]AC
HinfI 786 GATTC G^A[ACGT]TC
MslI 960 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1066 CAGCGG C[AC]G^C[GT]G
PmaCI 373 CACGTG CAC^GTG
PmlI 373 CACGTG CAC^GTG
SacI 745 GAGCTC GAGCT^C
ScaI 1025 AGTACT AGT^ACT
SmaI 740 CCCGGG CCC^GGG
StyI 524 CCTTGG C^C[AT][AT]GG
TfiI 786 GATTC G^A[AT]TC
Tsp509I 496 AATT ^AATT
XmaCI 740 CCCGGG C^CCGGG
XmaI 740 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 346 GTAC GT^AC
1026 GTAC
AlwI 642 GGATC GGATC(4/5)
734 GGATC
Asp700I 612 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1023 GAAGTACTTC
AvaII 140 GGACC G^G[AT]CC
819 GGTCC
AviII 251 TGCGCA TGC^GCA
401 TGCGCA
BanI 864 GGCACC G^G[CT][GA]CC
1113 GGTGCC
BanII 699 GGGCCC G[GA]GC[CT]^C
745 GAGCTC
BglI 277 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
433 GCCTTGCTGGC
BglII 614 AGATCT A^GATCT
629 AGATCT
BsmAI 409 GTCTC GTCTC(1/5)
421 GTCTC
BsmBI 408 CGTCTC CGTCTC(1/5)
420 CGTCTC
BsmFI 763 GTCCC GGGAC(10/14)
1058 GTCCC
EarI 673 CTCTTC CTCTTC(1/4)
1126 GAAGAG
FauI 236 CCCGC CCCGC(4/6)
1068 GCGGG
FspI 251 TGCGCA TGC^GCA
401 TGCGCA
HapII 138 CCGG C^CGG
741 CCGG
HpaII 138 CCGG C^CGG
741 CCGG
Ksp632I 673 CTCTTC CTCTTC(1/4)
1126 GAAGAG
MseI 41 TTAA T^TAA
77 TTAA
MspI 138 CCGG C^CGG
741 CCGG
RsaI 346 GTAC GT^AC
1026 GTAC
Tru9I 41 TTAA T^TAA
77 TTAA
XcmI 428 CCAATGCCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1121 CCACAGAAGAGGTGG
XmnI 612 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1023 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AspHI BalI BbvI
BcnI BmyI BsaAI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HphI ItaI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII