Restriction map for
pF1KB3963
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BalI BamHI BbeI BbrPI BciVI
BfaI BfrI BglI BlnI BsaAI
BsaWI BseAI BsiWI BspEI BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MaeI
MluI MluNI MroI MscI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI StuI XbaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1357 GACGTC GACGT^C
AccI 1636 GTCGAC GT^[AC][GT]AC
AccII 2070 CGCG CG^CG
AflIII 890 ACATGT A^C[GA][CT]GT
AhdI 1618 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 99 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1618 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2037 GTGCTC G[AT]GC[AT]^C
AspI 919 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2112 CCTCAGC CCTCAGC(-5/-2)
BlpI 2126 GCTTAGC GC^T[ACGT]AGC
BpmI 1723 CTGGAG CTGGAG(16/14)
Bpu1102I 2126 GCTTAGC GC^T[ACGT]AGC
BseRI 2110 CTCCTC GAGGAG(10/8)
BsgI 78 CTGCAC GTGCAG(16/14)
BsiEI 724 CGACCG CG[GA][CT]^CG
BsiHKAI 2037 GTGCTC G[AT]GC[AT]^C
BsmI 1497 GAATGC GAATGC(1/-1)
Bsp1407I 1649 TGTACA T^GTACA
BspHI 1052 TCATGA T^CATGA
BspLU11I 890 ACATGT A^CATGT
BsrBI 16 CCGCTC CCGCTC(-3/-3)
BsrFI 681 GCCGGT [GA]^CCGG[CT]
BsrGI 1649 TGTACA T^GTACA
BssSI 325 CTCGTG CACGAG(-5/-1)
BstUI 2070 CGCG CG^CG
BstXI 1558 CCAGCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2126 GCTTAGC GC^T[ACGT]AGC
Cfr10I 681 GCCGGT [GA]^CCGG[CT]
DraI 373 TTTAAA TTT^AAA
DsaI 1502 CCACGG C^C[GA][CT]GG
Eam1105I 1618 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 605 CCTTGG C^C[AT][AT]GG
FauI 1690 CCCGC CCCGC(4/6)
MfeI 1670 CAATTG C^AATTG
MunI 1670 CAATTG C^AATTG
MvnI 2070 CGCG CG^CG
NspI 890 ACATGT [GA]CATG^[CT]
PflFI 919 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 756 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1052 TCATGA T^CATGA
SalI 1636 GTCGAC G^TCGAC
SbfI 2042 CCTGCAGG CCTGCA^GG
SmaI 1219 CCCGGG CCC^GGG
Sse8387I 2042 CCTGCAGG CCTGCA^GG
SspBI 1649 TGTACA T^GTACA
SspI 1573 AATATT AAT^ATT
StyI 605 CCTTGG C^C[AT][AT]GG
SwaI 372 ATTTAAAT ATTT^AAAT
Tth111I 919 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 756 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1604 CCACAGCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1219 CCCGGG C^CCGGG
XmaI 1219 CCCGGG C^CCGGG
XmnI 99 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 867 GACGCC G[GA]^CG[CT]C
1357 GACGTC
ApaI 1419 GGGCCC GGGCC^C
2078 GGGCCC
BanI 684 GGTGCC G^G[CT][GA]CC
781 GGCACC
BbsI 1339 GAAGAC GAAGAC(2/6)
1426 GTCTTC
BcgI 479 GCAAGGTAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
665 GCAAAACTCTCG
BcnI 1219 CCCGG CC^[GC]GG
1220 CCGGG
BglII 1898 AGATCT A^GATCT
2019 AGATCT
BpuAI 1339 GAAGAC GAAGAC(2/6)
1426 GTCTTC
BsaBI 1050 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1822 GATAAGGATC
BsaHI 867 GACGCC G[GA]^CG[CT]C
1357 GACGTC
BsaI 646 GAGACC GGTCTC(1/5)
201 GGTCTC
BsmBI 588 CGTCTC CGTCTC(1/5)
1359 CGTCTC
BspDI 901 ATCGAT AT^CGAT
1057 ATCGAT
BstEII 770 GGTCACC G^GT[ACGT]ACC
1449 GGTGACC
BstPI 770 GGTCACC G^GT[ACGT]ACC
1449 GGTGACC
CfoI 943 GCGC GCG^C
2085 GCGC
ClaI 901 ATCGAT AT^CGAT
1057 ATCGAT
EaeI 753 CGGCCA [CT]^GGCC[GA]
1037 CGGCCA
EarI 1660 CTCTTC CTCTTC(1/4)
2119 CTCTTC
EcoNI 36 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
907 CCTGAGAAAGG
EcoO65I 770 GGTCACC G^GT[ACGT]ACC
1449 GGTGACC
EcoRI 127 GAATTC G^AATTC
2049 GAATTC
HgaI 867 GACGC GACGC(5/10)
587 GCGTC
HhaI 943 GCGC GCG^C
2085 GCGC
Hin1I 867 GACGCC G[GA]^CG[CT]C
1357 GACGTC
HinP1I 943 GCGC G^CGC
2085 GCGC
Ksp632I 1660 CTCTTC CTCTTC(1/4)
2119 CTCTTC
MamI 1050 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1822 GATAAGGATC
MspA1I 122 CAGCTG C[AC]G^C[GT]G
1607 CAGCTG
NciI 1219 CCCGG CC^[GC]GG
1220 CCGGG
PaeR7I 204 CTCGAG C^TCGAG
673 CTCGAG
PpuMI 1463 AGGACCT [GA]G^G[AT]CC[CT]
1553 AGGACCC
PvuII 122 CAGCTG CAG^CTG
1607 CAGCTG
XhoI 204 CTCGAG C^TCGAG
673 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BanII
BbvI BclI BmyI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII