Restriction map for
pF1KB3965
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BfaI BglI BlnI BlpI
Bpu1102I BseAI BsgI BsiEI BsiWI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI BstBI BstUI
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FbaI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MaeI MfeI MluI MroI MunI
MvnI NarI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 492 GACGTC GACGT^C
AcsI 320 AAATTC [GA]^AATT[CT]
AcyI 492 GACGTC G[GA]^CG[CT]C
AflII 778 CTTAAG C^TTAAG
AflIII 695 ACGTGT A^C[GA][CT]GT
AlwI 1596 GATCC GGATC(4/5)
AocI 1602 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1553 AGCGCT AGC^GCT
ApoI 320 AAATTC [GA]^AATT[CT]
BalI 1098 TGGCCA TGG^CCA
BciVI 1032 GGATAC GTATCC(6/5)
BcnI 2118 CCGGG CC^[GC]GG
BfrI 778 CTTAAG C^TTAAG
BglII 1808 AGATCT A^GATCT
BsaAI 244 TACGTA [CT]AC^GT[GA]
BsaBI 1809 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 492 GACGTC G[GA]^CG[CT]C
BsaWI 1589 ACCGGA [AT]^CCGG[AT]
BsmBI 74 CGTCTC CGTCTC(1/5)
Bsp1407I 551 TGTACA T^GTACA
BsrFI 6 GCCGGC [GA]^CCGG[CT]
BsrGI 551 TGTACA T^GTACA
BstEII 2169 GGTTACC G^GT[ACGT]ACC
BstPI 2169 GGTTACC G^GT[ACGT]ACC
Bsu36I 1602 CCTCAGG CC^T[ACGT]AGG
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
DrdI 106 GACTTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1712 CCATGG C^C[GA][CT]GG
EaeI 1098 TGGCCA [CT]^GGCC[GA]
Eco47III 1553 AGCGCT AGC^GCT
Eco81I 1602 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2169 GGTTACC G^GT[ACGT]ACC
FauI 1346 CCCGC CCCGC(4/6)
HaeII 1553 AGCGCT [GA]GCGC^[CT]
HgaI 1761 GACGC GACGC(5/10)
Hin1I 492 GACGTC G[GA]^CG[CT]C
MamI 1809 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1098 TGGCCA TGG^CCA
MscI 1098 TGGCCA TGG^CCA
NaeI 6 GCCGGC GCC^GGC
NciI 2118 CCGGG CC^[GC]GG
NcoI 1712 CCATGG C^CATGG
NgoMIV 6 GCCGGC G^CCGGC
NspI 1612 GCATGT [GA]CATG^[CT]
PpuMI 50 GGGACCT [GA]G^G[AT]CC[CT]
ScaI 1439 AGTACT AGT^ACT
SnaBI 244 TACGTA TAC^GTA
SspBI 551 TGTACA T^GTACA
SspI 229 AATATT AAT^ATT
XcmI 1976 CCAAAATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BanII 370 GAGCTC G[GA]GC[CT]^C
1105 GAGCTC
BpmI 736 CTGGAG CTGGAG(16/14)
1549 CTGGAG
BsaI 1723 GAGACC GGTCTC(1/5)
2191 GGTCTC
BsmI 507 GAATGC GAATGC(1/-1)
945 GAATGC
BsrDI 527 GCAATG GCAATG(2/0)
1292 GCAATG
Bst1107I 670 GTATAC GTA^TAC
1527 GTATAC
BstAPI 21 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
918 GCAGAGCTTGC
BstYI 1595 AGATCC [GA]^GATC[CT]
1808 AGATCT
BstZ17I 670 GTATAC GTA^TAC
1527 GTATAC
DraII 50 GGGACCT [GA]G^G[ACGT]CC[CT]
1470 AGGCCCT
EcoNI 963 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2051 CCTTCCAGAGG
EcoO109I 50 GGGACCT [GA]G^G[ACGT]CC[CT]
1470 AGGCCCT
MflI 1595 AGATCC [GA]^GATC[CT]
1808 AGATCT
MspA1I 22 CAGCTG C[AC]G^C[GT]G
271 CAGCTG
NdeI 1164 CATATG CA^TATG
1902 CATATG
PflMI 1454 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2196 CCACAACATGG
PstI 19 CTGCAG CTGCA^G
265 CTGCAG
PvuII 22 CAGCTG CAG^CTG
271 CAGCTG
SacI 370 GAGCTC GAGCT^C
1105 GAGCTC
StuI 732 AGGCCT AGG^CCT
2048 AGGCCT
TaqI 38 TCGA T^CGA
1457 TCGA
TthHB8I 38 TCGA T^CGA
1457 TCGA
Van91I 1454 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2196 CCACAACATGG
XhoII 1595 AGATCC [GA]^GATC[CT]
1808 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccI AciI AfaI AluI AlwNI
AspHI AvaII BbsI BbvI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI