################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI AlwI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI Sau96I SbfI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 94 AAATTT [GA]^AATT[CT] AflII 109 CTTAAG C^TTAAG Alw44I 218 GTGCAC G^TGCAC AlwNI 20 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 218 GTGCAC G^TGCAC ApoI 94 AAATTT [GA]^AATT[CT] AseI 354 ATTAAT AT^TAAT AsnI 354 ATTAAT AT^TAAT BanII 329 GAGCTC G[GA]GC[CT]^C BbvI 471 GCTGC GCAGC(8/12) BciVI 185 GTATCC GTATCC(6/5) BfaI 349 CTAG C^TAG BfrI 109 CTTAAG C^TTAAG BpmI 332 CTCCAG CTGGAG(16/14) BsaJI 321 CCTTGG C^C[ACGT][ACGT]GG BsmI 512 GCATTC GAATGC(1/-1) BsrDI 307 CATTGC GCAATG(2/0) BssKI 286 CCTGG ^CC[ACGT]GG BstF5I 227 CATCC GGATG(2/0) BstNI 286 CCTGG CC^[AT]GG EcoRII 286 CCTGG ^CC[AT]GG EcoT14I 321 CCTTGG C^C[AT][AT]GG FokI 227 CATCC GGATG(9/13) HinfI 484 GATTC G^A[ACGT]TC MaeI 349 CTAG C^TAG MnlI 103 GAGG CCTC(7/6) MvaI 286 CCTGG CC^[AT]GG MwoI 311 GCGTTGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PshBI 354 ATTAAT AT^TAAT SacI 329 GAGCTC GAGCT^C ScaI 336 AGTACT AGT^ACT ScrFI 286 CCTGG CC^[ACGT]GG SexAI 285 ACCTGGT A^CC[AT]GGT SfaNI 382 GATGC GCATC(5/9) SmlI 109 CTTAAG C^T[CT][GA]AG StuI 318 AGGCCT AGG^CCT StyI 321 CCTTGG C^C[AT][AT]GG TaqI 6 TCGA T^CGA TfiI 484 GATTC G^A[AT]TC TseI 471 GCTGC G^C[AT]GC Tsp45I 34 GTCAC ^GT[GC]AC TthHB8I 6 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 337 GTAC GT^AC 388 GTAC AspHI 218 GTGCAC G[AT]GC[AT]^C 329 GAGCTC BmyI 218 GTGCAC G[AGT]GC[ACT]^C 329 GAGCTC BsiHKAI 218 GTGCAC G[AT]GC[AT]^C 329 GAGCTC Bsp1286I 218 GTGCAC G[AGT]GC[ACT]^C 329 GAGCTC BsrI 248 ACTGG ACTGG(1/-1) 334 CCAGT Cac8I 316 GCAGGC GC[ACGT]^[ACGT]GC 428 GCAAGC HaeIII 203 GGCC GG^CC 319 GGCC MaeIII 34 GTCAC ^GT[ACGT]AC 124 GTTAC MboII 280 GAAGA GAAGA(8/7) 379 GAAGA NlaIII 48 CATG CATG^ 190 CATG RsaI 337 GTAC GT^AC 388 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AciI AluI Fnu4HI ItaI MseI Tru9I Tsp509I