##################################
# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DdeI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI Sau3AI Sau96I SbfI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 94 AAATTT [GA]^AATT[CT]
AflII 109 CTTAAG C^TTAAG
Alw44I 218 GTGCAC G^TGCAC
AlwNI 20 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 218 GTGCAC G^TGCAC
ApoI 94 AAATTT [GA]^AATT[CT]
AseI 354 ATTAAT AT^TAAT
AsnI 354 ATTAAT AT^TAAT
BanII 329 GAGCTC G[GA]GC[CT]^C
BbvI 471 GCTGC GCAGC(8/12)
BciVI 185 GTATCC GTATCC(6/5)
BfaI 349 CTAG C^TAG
BfrI 109 CTTAAG C^TTAAG
BpmI 332 CTCCAG CTGGAG(16/14)
BsaJI 321 CCTTGG C^C[ACGT][ACGT]GG
BsmI 512 GCATTC GAATGC(1/-1)
BsrDI 307 CATTGC GCAATG(2/0)
BssKI 286 CCTGG ^CC[ACGT]GG
BstF5I 227 CATCC GGATG(2/0)
BstNI 286 CCTGG CC^[AT]GG
EcoRII 286 CCTGG ^CC[AT]GG
EcoT14I 321 CCTTGG C^C[AT][AT]GG
FokI 227 CATCC GGATG(9/13)
HinfI 484 GATTC G^A[ACGT]TC
MaeI 349 CTAG C^TAG
MnlI 103 GAGG CCTC(7/6)
MvaI 286 CCTGG CC^[AT]GG
MwoI 311 GCGTTGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshBI 354 ATTAAT AT^TAAT
SacI 329 GAGCTC GAGCT^C
ScaI 336 AGTACT AGT^ACT
ScrFI 286 CCTGG CC^[ACGT]GG
SexAI 285 ACCTGGT A^CC[AT]GGT
SfaNI 382 GATGC GCATC(5/9)
SmlI 109 CTTAAG C^T[CT][GA]AG
StuI 318 AGGCCT AGG^CCT
StyI 321 CCTTGG C^C[AT][AT]GG
TaqI 6 TCGA T^CGA
TfiI 484 GATTC G^A[AT]TC
TseI 471 GCTGC G^C[AT]GC
Tsp45I 34 GTCAC ^GT[GC]AC
TthHB8I 6 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 337 GTAC GT^AC
388 GTAC
AspHI 218 GTGCAC G[AT]GC[AT]^C
329 GAGCTC
BmyI 218 GTGCAC G[AGT]GC[ACT]^C
329 GAGCTC
BsiHKAI 218 GTGCAC G[AT]GC[AT]^C
329 GAGCTC
Bsp1286I 218 GTGCAC G[AGT]GC[ACT]^C
329 GAGCTC
BsrI 248 ACTGG ACTGG(1/-1)
334 CCAGT
Cac8I 316 GCAGGC GC[ACGT]^[ACGT]GC
428 GCAAGC
HaeIII 203 GGCC GG^CC
319 GGCC
MaeIII 34 GTCAC ^GT[ACGT]AC
124 GTTAC
MboII 280 GAAGA GAAGA(8/7)
379 GAAGA
NlaIII 48 CATG CATG^
190 CATG
RsaI 337 GTAC GT^AC
388 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AciI AluI Fnu4HI ItaI MseI
Tru9I Tsp509I