Restriction map for
pF1KB4102
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AvrII
BamHI BbrPI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrDI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI HincII HindII HindIII HpaI
KpnI Ksp632I MamI MfeI MroI
MseI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SspI
SwaI TfiI Tru9I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 2116 GAATTC [GA]^AATT[CT]
AocI 1091 CCTCAGG CC^T[ACGT]AGG
ApoI 2116 GAATTC [GA]^AATT[CT]
AspHI 1549 GAGCAC G[AT]GC[AT]^C
AspI 306 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 34 GAGCCC G[GA]GC[CT]^C
BbeI 1372 GGCGCC GGCGC^C
BbsI 1510 GTCTTC GAAGAC(2/6)
BglII 1730 AGATCT A^GATCT
BpuAI 1510 GTCTTC GAAGAC(2/6)
BsaAI 139 TACGTG [CT]AC^GT[GA]
BsaWI 296 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1549 GAGCAC G[AT]GC[AT]^C
BsmBI 130 GAGACG CGTCTC(1/5)
Bsp1407I 219 TGTACA T^GTACA
BsrGI 219 TGTACA T^GTACA
BstXI 38 CCAACGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1091 CCTCAGG CC^T[ACGT]AGG
DrdI 1684 GACCAGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1091 CCTCAGG CC^T[ACGT]AGG
EcoRI 2116 GAATTC G^AATTC
EcoT22I 1969 ATGCAT ATGCA^T
KasI 1372 GGCGCC G^GCGCC
KspI 56 CCGCGG CCGC^GG
MluI 17 ACGCGT A^CGCGT
NaeI 1017 GCCGGC GCC^GGC
NarI 1372 GGCGCC GG^CGCC
NgoMIV 1017 GCCGGC G^CCGGC
NsiI 1969 ATGCAT ATGCA^T
PflFI 306 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 369 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 56 CCGCGG CCGC^GG
SfoI 1372 GGCGCC GGC^GCC
SphI 1971 GCATGC GCATG^C
SspBI 219 TGTACA T^GTACA
Tth111I 306 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 369 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1376 CCATGTGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1162 GACGCC G[GA]^CG[CT]C
1372 GGCGCC
AflIII 17 ACGCGT A^C[GA][CT]GT
1838 ACGTGT
AlwI 288 GATCC GGATC(4/5)
486 GATCC
AviII 1781 TGCGCA TGC^GCA
1946 TGCGCA
BfaI 79 CTAG C^TAG
1219 CTAG
BsaHI 1162 GACGCC G[GA]^CG[CT]C
1372 GGCGCC
BsgI 1666 CTGCAC GTGCAG(16/14)
1380 GTGCAG
BsrBI 24 CCGCTC CCGCTC(-3/-3)
427 GAGCGG
BsrFI 1017 GCCGGC [GA]^CCGG[CT]
2012 ACCGGC
BstYI 485 AGATCC [GA]^GATC[CT]
1730 AGATCT
Cfr10I 1017 GCCGGC [GA]^CCGG[CT]
2012 ACCGGC
EcoNI 174 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2049 CCTGAATGAGG
FspI 1781 TGCGCA TGC^GCA
1946 TGCGCA
HaeII 539 AGCGCC [GA]GCGC^[CT]
1372 GGCGCC
HgaI 1162 GACGC GACGC(5/10)
2042 GACGC
Hin1I 1162 GACGCC G[GA]^CG[CT]C
1372 GGCGCC
HinfI 272 GAGTC G^A[ACGT]TC
1294 GAGTC
MaeI 79 CTAG C^TAG
1219 CTAG
MaeII 140 ACGT A^CGT
1838 ACGT
MflI 485 AGATCC [GA]^GATC[CT]
1730 AGATCT
NcoI 1505 CCATGG C^CATGG
1517 CCATGG
NspI 1448 ACATGC [GA]CATG^[CT]
1971 GCATGC
PleI 272 GAGTC GAGTC(4/5)
1294 GAGTC
PpuMI 1043 AGGTCCT [GA]G^G[AT]CC[CT]
1070 AGGACCT
SfiI 555 GGCCCTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1305 GGCCTTGGTGGCC
SmlI 1360 CTCAAG C^T[CT][GA]AG
1822 CTCAAG
StuI 474 AGGCCT AGG^CCT
1007 AGGCCT
Tsp509I 965 AATT ^AATT
2117 AATT
XhoII 485 AGATCC [GA]^GATC[CT]
1730 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BalI BanI BbvCI BbvI
BcgI BcnI BglI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI Sse8387I StyI TaqI TseI
Tsp45I TspRI TthHB8I