Restriction map for
pF1KB4144
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstNI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRII EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI Ksp632I
MaeI MaeII MamI MboI MboII
MfeI MflI MluI MroI MunI
MvaI NaeI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AfaI 330 GTAC GT^AC
AlwNI 115 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 365 CCCGGG C^[CT]CG[GA]G
AvaII 370 GGACC G^G[AT]CC
BalI 392 TGGCCA TGG^CCA
BanI 378 GGCGCC G^G[CT][GA]CC
BanII 430 GAGCTC G[GA]GC[CT]^C
BbeI 378 GGCGCC GGCGC^C
BlpI 69 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 69 GCTCAGC GC^T[ACGT]AGC
BsaI 127 GGTCTC GGTCTC(1/5)
BsiYI 359 CCGTGGCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 359 CCGTGGCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 128 GTCTC GTCTC(1/5)
BsmFI 369 GGGAC GGGAC(10/14)
BsoBI 365 CCCGGG C^[CT]CG[GA]G
BspMI 119 ACCTGC ACCTGC(4/8)
BsrBI 238 GAGCGG CCGCTC(-3/-3)
BssHII 352 GCGCGC G^CGCGC
BstEII 144 GGTGACC G^GT[ACGT]ACC
BstF5I 464 CATCC GGATG(2/0)
BstPI 144 GGTGACC G^GT[ACGT]ACC
CelII 69 GCTCAGC GC^T[ACGT]AGC
DdeI 70 CTCAG C^T[ACGT]AG
DraII 369 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 369 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 144 GGTGACC G^GT[ACGT]ACC
FokI 464 CATCC GGATG(9/13)
KasI 378 GGCGCC G^GCGCC
KspI 183 CCGCGG CCGC^GG
MluNI 392 TGGCCA TGG^CCA
MscI 392 TGGCCA TGG^CCA
MseI 31 TTAA T^TAA
NarI 378 GGCGCC GG^CGCC
NspI 296 GCATGC [GA]CATG^[CT]
PpuMI 369 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 198 CAGCTG CAG^CTG
RsaI 330 GTAC GT^AC
SacI 430 GAGCTC GAGCT^C
SacII 183 CCGCGG CCGC^GG
SfoI 378 GGCGCC GGC^GCC
SmaI 365 CCCGGG CCC^GGG
SmlI 175 CTCAAG C^T[CT][GA]AG
SphI 296 GCATGC GCATG^C
Tru9I 31 TTAA T^TAA
XmaCI 365 CCCGGG C^CCGGG
XmaI 365 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 313 GACGCC G[GA]^CG[CT]C
378 GGCGCC
AspHI 67 GTGCTC G[AT]GC[AT]^C
430 GAGCTC
BpmI 235 CTGGAG CTGGAG(16/14)
304 CTGGAG
BsaHI 313 GACGCC G[GA]^CG[CT]C
378 GGCGCC
BsiHKAI 67 GTGCTC G[AT]GC[AT]^C
430 GAGCTC
Cfr13I 363 GGCCC G^G[ACGT]CC
370 GGACC
DsaI 183 CCGCGG C^C[GA][CT]GG
359 CCGTGG
EaeI 194 CGGCCA [CT]^GGCC[GA]
392 TGGCCA
EcoT14I 134 CCAAGG C^C[AT][AT]GG
386 CCAAGG
HaeII 336 GGCGCT [GA]GCGC^[CT]
378 GGCGCC
HapII 64 CCGG C^CGG
366 CCGG
Hin1I 313 GACGCC G[GA]^CG[CT]C
378 GGCGCC
HpaII 64 CCGG C^CGG
366 CCGG
MaeIII 145 GTGAC ^GT[ACGT]AC
217 GTGAC
MslI 93 CATGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
441 CACCTCCGTG
MspA1I 183 CCGCGG C[AC]G^C[GT]G
198 CAGCTG
MspI 64 CCGG C^CGG
366 CCGG
NlaIII 93 CATG CATG^
297 CATG
NlaIV 369 GGGACC GG[ACGT]^[ACGT]CC
378 GGCGCC
PstI 256 CTGCAG CTGCA^G
340 CTGCAG
Sau96I 363 GGCCC G^G[ACGT]CC
370 GGACC
SfaNI 206 GCATC GCATC(5/9)
463 GCATC
SfcI 256 CTGCAG C^T[GA][CT]AG
340 CTGCAG
StyI 134 CCAAGG C^C[AT][AT]GG
386 CCAAGG
Tsp45I 145 GTGAC ^GT[GC]AC
217 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BmyI BsaJI BseRI Bsp1286I BssKI
BstUI Cac8I CfoI FauI Fnu4HI
HaeIII HgaI HhaI HinP1I HphI
ItaI MnlI MvnI MwoI NciI
ScrFI TseI