Restriction map for
pF1KB4158
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BssHII BstAPI BstBI BstUI Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SwaI Tth111I Van91I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AclI 821 AACGTT AA^CGTT
AvaI 1449 CTCGGG C^[CT]CG[GA]G
BanII 985 GAGCCC G[GA]GC[CT]^C
BciVI 630 GTATCC GTATCC(6/5)
BcnI 1413 CCGGG CC^[GC]GG
BglI 787 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 476 GCTTAGC GC^T[ACGT]AGC
BmyI 985 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 476 GCTTAGC GC^T[ACGT]AGC
BsaAI 557 TACGTA [CT]AC^GT[GA]
BseRI 52 GAGGAG GAGGAG(10/8)
BsgI 18 CTGCAC GTGCAG(16/14)
BsiYI 17 CCTGCACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 17 CCTGCACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 1449 CTCGGG C^[CT]CG[GA]G
Bsp1286I 985 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 1296 TGTACA T^GTACA
BspHI 228 TCATGA T^CATGA
BspMI 16 ACCTGC ACCTGC(4/8)
BsrGI 1296 TGTACA T^GTACA
BssSI 1397 CTCGTG CACGAG(-5/-1)
Bst1107I 347 GTATAC GTA^TAC
BstEII 1454 GGTCACC G^GT[ACGT]ACC
BstPI 1454 GGTCACC G^GT[ACGT]ACC
BstXI 634 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 347 GTATAC GTA^TAC
CelII 476 GCTTAGC GC^T[ACGT]AGC
DraII 404 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1377 GACAATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 808 CCATGG C^C[GA][CT]GG
EcoNI 17 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 404 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1454 GGTCACC G^GT[ACGT]ACC
EcoRV 536 GATATC GAT^ATC
HaeIII 1168 GGCC GG^CC
HapII 1413 CCGG C^CGG
HpaII 1413 CCGG C^CGG
MfeI 1052 CAATTG C^AATTG
MspA1I 452 CAGCTG C[AC]G^C[GT]G
MspI 1413 CCGG C^CGG
MunI 1052 CAATTG C^AATTG
NciI 1413 CCGGG CC^[GC]GG
NcoI 808 CCATGG C^CATGG
NheI 433 GCTAGC G^CTAGC
NspI 595 GCATGT [GA]CATG^[CT]
PpuMI 404 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 821 AACGTT AA^CGTT
PvuII 452 CAGCTG CAG^CTG
RcaI 228 TCATGA T^CATGA
SexAI 22 ACCAGGT A^CC[AT]GGT
SnaBI 557 TACGTA TAC^GTA
SspBI 1296 TGTACA T^GTACA
SspI 214 AATATT AAT^ATT
StuI 1167 AGGCCT AGG^CCT
XbaI 360 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 347 GTATAC GT^[AC][GT]AC
862 GTAGAC
AciI 1319 CCGC CCGC(-3/-1)
1410 CCGC
AvaII 405 GGACC G^G[AT]CC
498 GGACC
BanI 904 GGTGCC G^G[CT][GA]CC
1406 GGCACC
BbsI 298 GAAGAC GAAGAC(2/6)
649 GAAGAC
BpuAI 298 GAAGAC GAAGAC(2/6)
649 GAAGAC
BsmAI 856 GAGAC GTCTC(1/5)
1393 GAGAC
BsmI 1367 GAATGC GAATGC(1/-1)
70 GCATTC
BstYI 564 AGATCC [GA]^GATC[CT]
812 GGATCT
Cfr13I 405 GGACC G^G[ACGT]CC
498 GGACC
EarI 244 GAAGAG CTCTTC(1/4)
1390 GAAGAG
EcoT14I 443 CCAAGG C^C[AT][AT]GG
808 CCATGG
HincII 818 GTCAAC GT[CT]^[GA]AC
1029 GTCAAC
HindII 818 GTCAAC GT[CT]^[GA]AC
1029 GTCAAC
HindIII 145 AAGCTT A^AGCTT
1190 AAGCTT
Ksp632I 244 GAAGAG CTCTTC(1/4)
1390 GAAGAG
MaeII 558 ACGT A^CGT
822 ACGT
MflI 564 AGATCC [GA]^GATC[CT]
812 GGATCT
MseI 371 TTAA T^TAA
881 TTAA
MslI 382 CACCTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
704 CACCAGAATG
MwoI 787 GCCTCAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1321 GCCTGCATAGC
Sau96I 405 GGACC G^G[ACGT]CC
498 GGACC
SfcI 321 CTACAG C^T[GA][CT]AG
860 CTGTAG
StyI 443 CCAAGG C^C[AT][AT]GG
808 CCATGG
Tru9I 371 TTAA T^TAA
881 TTAA
XhoII 564 AGATCC [GA]^GATC[CT]
812 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BpmI BsaJI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI SfaNI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI