Restriction map for
pF1KB4159
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 349 AAATTT [GA]^AATT[CT]
AfaI 162 GTAC GT^AC
AgeI 65 ACCGGT A^CCGGT
AlwI 372 GATCC GGATC(4/5)
ApoI 349 AAATTT [GA]^AATT[CT]
AvaII 63 GGACC G^G[AT]CC
BanII 150 GGGCTC G[GA]GC[CT]^C
BbsI 103 GAAGAC GAAGAC(2/6)
BmyI 150 GGGCTC G[AGT]GC[ACT]^C
BpuAI 103 GAAGAC GAAGAC(2/6)
BsaWI 65 ACCGGT [AT]^CCGG[AT]
Bsp1286I 150 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 161 TGTACA T^GTACA
BsrFI 65 ACCGGT [GA]^CCGG[CT]
BsrGI 161 TGTACA T^GTACA
BstF5I 249 CATCC GGATG(2/0)
CfoI 230 GCGC GCG^C
Cfr10I 65 ACCGGT [GA]^CCGG[CT]
Cfr13I 63 GGACC G^G[ACGT]CC
EcoT14I 278 CCAAGG C^C[AT][AT]GG
FokI 249 CATCC GGATG(9/13)
HapII 66 CCGG C^CGG
HhaI 230 GCGC GCG^C
HinP1I 230 GCGC G^CGC
HpaII 66 CCGG C^CGG
HphI 267 GGTGA GGTGA(8/7)
MseI 403 TTAA T^TAA
MspI 66 CCGG C^CGG
NlaIV 151 GGCTCC GG[ACGT]^[ACGT]CC
NspI 310 GCATGT [GA]CATG^[CT]
PinAI 65 ACCGGT A^CCGGT
RsaI 162 GTAC GT^AC
Sau96I 63 GGACC G^G[ACGT]CC
SfaNI 270 GATGC GCATC(5/9)
SfcI 237 CTGTAG C^T[GA][CT]AG
SspBI 161 TGTACA T^GTACA
StyI 278 CCAAGG C^C[AT][AT]GG
Tru9I 403 TTAA T^TAA
Tsp45I 128 GTGAC ^GT[GC]AC
Tsp509I 350 AATT ^AATT
TspRI 83 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbvI 44 GCTGC GCAGC(8/12)
232 GCTGC
BsaJI 278 CCAAGG C^C[ACGT][ACGT]GG
425 CCCAGG
BsmAI 194 GAGAC GTCTC(1/5)
344 GAGAC
BsrI 84 ACTGG ACTGG(1/-1)
95 ACTGG
BssKI 297 CCTGG ^CC[ACGT]GG
426 CCAGG
BstNI 297 CCTGG CC^[AT]GG
426 CCAGG
DdeI 6 CTCAG C^T[ACGT]AG
202 CTGAG
DpnII 57 GATC ^GATC
372 GATC
EcoRII 297 CCTGG ^CC[AT]GG
426 CCAGG
Fnu4HI 44 GCTGC GC^[ACGT]GC
232 GCTGC
ItaI 44 GCTGC GC^[ACGT]GC
232 GCTGC
MaeIII 128 GTGAC ^GT[ACGT]AC
217 GTAAC
MboI 57 GATC ^GATC
372 GATC
MboII 103 GAAGA GAAGA(8/7)
72 TCTTC
MvaI 297 CCTGG CC^[AT]GG
426 CCAGG
NdeII 57 GATC ^GATC
372 GATC
Sau3AI 57 GATC ^GATC
372 GATC
ScrFI 297 CCTGG CC^[ACGT]GG
426 CCAGG
TseI 44 GCTGC G^C[AT]GC
232 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
AluI Cac8I MnlI NlaIII