Restriction map for
pF1KB4360
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# NO CUT ENZYMES ( 92 enzymes) #
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Acc65I AclI AflII AflIII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbsI BbvCI BclI
BfrI BglII BlnI BpuAI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoNI EcoRI EcoRV FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MflI MluI MunI
NcoI NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1324 GACGTC GACGT^C
AccIII 2363 TCCGGA T^CCGGA
AlwI 1111 GATCC GGATC(4/5)
AlwNI 443 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1542 CCTCAGG CC^T[ACGT]AGG
Aor51HI 594 AGCGCT AGC^GCT
ApaI 2184 GGGCCC GGGCC^C
AviII 1868 TGCGCA TGC^GCA
BbeI 1704 GGCGCC GGCGC^C
BbrPI 1972 CACGTG CAC^GTG
BcgI 2074 GCATTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1683 GTATCC GTATCC(6/5)
BpmI 1053 CTCCAG CTGGAG(16/14)
BsaAI 1972 CACGTG [CT]AC^GT[GA]
BseAI 2363 TCCGGA T^CCGGA
BsmI 194 GAATGC GAATGC(1/-1)
BspEI 2363 TCCGGA T^CCGGA
BssHII 188 GCGCGC G^CGCGC
Bsu36I 1542 CCTCAGG CC^T[ACGT]AGG
EagI 1733 CGGCCG C^GGCCG
EarI 2095 CTCTTC CTCTTC(1/4)
EclXI 1733 CGGCCG C^GGCCG
Eco47III 594 AGCGCT AGC^GCT
Eco52I 1733 CGGCCG C^GGCCG
Eco81I 1542 CCTCAGG CC^T[ACGT]AGG
EcoT22I 196 ATGCAT ATGCA^T
FspI 1868 TGCGCA TGC^GCA
KasI 1704 GGCGCC G^GCGCC
Ksp632I 2095 CTCTTC CTCTTC(1/4)
KspI 1602 CCGCGG CCGC^GG
MroI 2363 TCCGGA T^CCGGA
NaeI 2278 GCCGGC GCC^GGC
NarI 1704 GGCGCC GG^CGCC
NdeI 818 CATATG CA^TATG
NgoMIV 2278 GCCGGC G^CCGGC
NheI 12 GCTAGC G^CTAGC
NsiI 196 ATGCAT ATGCA^T
NspI 1844 GCATGT [GA]CATG^[CT]
PaeR7I 797 CTCGAG C^TCGAG
PmaCI 1972 CACGTG CAC^GTG
PmlI 1972 CACGTG CAC^GTG
PpuMI 2224 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 151 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1810 CAGCTG CAG^CTG
SacII 1602 CCGCGG CCGC^GG
SapI 2094 GCTCTTC GCTCTTC(1/4)
SbfI 1566 CCTGCAGG CCTGCA^GG
SfoI 1704 GGCGCC GGC^GCC
Sse8387I 1566 CCTGCAGG CCTGCA^GG
XcmI 1904 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 797 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 1044 GTCTAC GT^[AC][GT]AC
2059 GTCTAC
BalI 134 TGGCCA TGG^CCA
176 TGGCCA
BglI 136 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1564 GCCCTGCAGGC
BsaI 1317 GGTCTC GGTCTC(1/5)
1506 GGTCTC
BsaWI 2263 ACCGGA [AT]^CCGG[AT]
2363 TCCGGA
BseRI 607 GAGGAG GAGGAG(10/8)
2142 GAGGAG
BspMI 1487 ACCTGC ACCTGC(4/8)
2006 ACCTGC
BsrFI 241 ACCGGC [GA]^CCGG[CT]
2278 GCCGGC
BstEII 1452 GGTCACC G^GT[ACGT]ACC
1752 GGTGACC
BstPI 1452 GGTCACC G^GT[ACGT]ACC
1752 GGTGACC
BstXI 1757 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2325 CCAGGGCGCTGG
Cfr10I 241 ACCGGC [GA]^CCGG[CT]
2278 GCCGGC
DsaI 1602 CCGCGG C^C[GA][CT]GG
1823 CCACGG
EcoO65I 1452 GGTCACC G^GT[ACGT]ACC
1752 GGTGACC
EcoT14I 846 CCAAGG C^C[AT][AT]GG
1757 CCAAGG
HgaI 1654 GACGC GACGC(5/10)
1897 GACGC
MluNI 134 TGGCCA TGG^CCA
176 TGGCCA
MscI 134 TGGCCA TGG^CCA
176 TGGCCA
SacI 217 GAGCTC GAGCT^C
619 GAGCTC
SexAI 1748 ACCTGGT A^CC[AT]GGT
2063 ACCTGGT
SfaNI 808 GATGC GCATC(5/9)
2199 GCATC
SmaI 1727 CCCGGG CCC^GGG
1831 CCCGGG
SmlI 797 CTCGAG C^T[CT][GA]AG
1816 CTCAAG
StyI 846 CCAAGG C^C[AT][AT]GG
1757 CCAAGG
XmaCI 1727 CCCGGG C^CCGGG
1831 CCCGGG
XmaI 1727 CCCGGG C^CCGGG
1831 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BlpI BmyI Bpu1102I BsaHI BsaJI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfcI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I