##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AatII       AccI        AclI        AflII       AgeI        
AhdI        AscI        AseI        AsnI        AspEI       
AspI        BclI        BfrI        BsiWI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CpoI        
DraI        Eam1105I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        MfeI        MluI        MunI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SalI        SfuI        SgrAI       
SnaBI       SspI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I       1796  GGTACC               G^GTACC
AccIII       3620  TCCGGA               T^CCGGA
AcsI         5713  AAATTC               [GA]^AATT[CT]
AflIII       5063  ACATGT               A^C[GA][CT]GT
AocI         4966  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      6188  AGCGCT               AGC^GCT
ApoI         5713  AAATTC               [GA]^AATT[CT]
Asp718I      1796  GGTACC               G^GTACC
AviII         323  TGCGCA               TGC^GCA
AvrII        4398  CCTAGG               C^CTAGG
BamHI        3617  GGATCC               G^GATCC
BbrPI        5170  CACGTG               CAC^GTG
BciVI        3438  GGATAC               GTATCC(6/5)
BlnI         4398  CCTAGG               C^CTAGG
BsaAI        5170  CACGTG               [CT]AC^GT[GA]
BsaBI         321  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        3620  TCCGGA               T^CCGGA
BsmBI        4468  GAGACG               CGTCTC(1/5)
BspDI        3994  ATCGAT               AT^CGAT
BspEI        3620  TCCGGA               T^CCGGA
BspLU11I     5063  ACATGT               A^CATGT
BsrDI        1294  GCAATG               GCAATG(2/0)
BssHII       2113  GCGCGC               G^CGCGC
BssSI        1330  CTCGTG               CACGAG(-5/-1)
Bsu36I       4966  CCTGAGG              CC^T[ACGT]AGG
ClaI         3994  ATCGAT               AT^CGAT
Eco47III     6188  AGCGCT               AGC^GCT
Eco81I       4966  CCTGAGG              CC^T[ACGT]AGG
FspI          323  TGCGCA               TGC^GCA
KpnI         1796  GGTACC               GGTAC^C
MamI          321  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         3620  TCCGGA               T^CCGGA
NaeI         6507  GCCGGC               GCC^GGC
NdeI         5594  CATATG               CA^TATG
NgoMIV       6507  GCCGGC               G^CCGGC
NheI          927  GCTAGC               G^CTAGC
PmaCI        5170  CACGTG               CAC^GTG
PmlI         5170  CACGTG               CAC^GTG
ScaI         4763  AGTACT               AGT^ACT
SexAI        2800  ACCAGGT              A^CC[AT]GGT
SpeI         1161  ACTAGT               A^CTAGT
Tsp509I      5714  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

Alw44I       2866  GTGCAC               G^TGCAC
             5509  GTGCAC              
ApaLI        2866  GTGCAC               G^TGCAC
             5509  GTGCAC              
BglII        1973  AGATCT               A^GATCT
             2084  AGATCT              
BsaWI        1283  ACCGGA               [AT]^CCGG[AT]
             3620  TCCGGA              
BspHI        4547  TCATGA               T^CATGA
             5822  TCATGA              
BstXI        2732  CCAACAGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6662  CCAAGAACCTGG        
KspI         4594  CCGCGG               CCGC^GG
             6514  CCGCGG              
RcaI         4547  TCATGA               T^CATGA
             5822  TCATGA              
SacII        4594  CCGCGG               CCGC^GG
             6514  CCGCGG              


#########################################
# 3 OR MORE CUT ENZYMES (  152 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbsI        BbvCI       BbvI        BcgI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspMI       BsrBI       
BsrFI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PaeR7I      PflMI       
PleI        PpuMI       PshAI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
SbfI        ScrFI       SfaNI       SfcI        SfiI        
SfoI        SmaI        SmlI        SphI        Sse8387I    
SspBI       StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      TspRI       TthHB8I     
Van91I      XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI