Restriction map for
pF1KB4505
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# NO CUT ENZYMES ( 79 enzymes) #
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Acc65I AccIII AclI AflII AlwNI
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BciVI BclI BfrI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BspEI BspHI BsrBI BssHII
Bst1107I BstBI BstXI BstZ17I CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI ScaI SexAI
SfiI SfuI SpeI SphI SspI
SwaI TfiI XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 2452 GTCTAC GT^[AC][GT]AC
AgeI 192 ACCGGT A^CCGGT
AhdI 277 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3231 CCTGAGG CC^T[ACGT]AGG
Asp700I 652 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 277 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2416 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 575 TGGCCA TGG^CCA
BbvCI 210 CCTCAGC CCTCAGC(-5/-2)
BcgI 3063 CGACGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 631 CTAG C^TAG
BlpI 867 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 867 GCTCAGC GC^T[ACGT]AGC
BsaWI 192 ACCGGT [AT]^CCGG[AT]
Bsp1407I 2024 TGTACA T^GTACA
BspDI 1477 ATCGAT AT^CGAT
BspLU11I 947 ACATGT A^CATGT
BsrDI 1748 GCAATG GCAATG(2/0)
BsrGI 2024 TGTACA T^GTACA
Bsu36I 3231 CCTGAGG CC^T[ACGT]AGG
CelII 867 GCTCAGC GC^T[ACGT]AGC
ClaI 1477 ATCGAT AT^CGAT
DraI 1036 TTTAAA TTT^AAA
Eam1105I 277 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2275 CTCTTC CTCTTC(1/4)
Eco81I 3231 CCTGAGG CC^T[ACGT]AGG
HincII 1831 GTCAAC GT[CT]^[GA]AC
HindII 1831 GTCAAC GT[CT]^[GA]AC
Ksp632I 2275 CTCTTC CTCTTC(1/4)
MaeI 631 CTAG C^TAG
MluNI 575 TGGCCA TGG^CCA
MscI 575 TGGCCA TGG^CCA
MseI 1037 TTAA T^TAA
PflFI 2416 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 192 ACCGGT A^CCGGT
PshAI 2437 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 547 CTGCAG CTGCA^G
PvuII 1008 CAGCTG CAG^CTG
SbfI 546 CCTGCAGG CCTGCA^GG
SgrAI 191 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 3043 TACGTA TAC^GTA
Sse8387I 546 CCTGCAGG CCTGCA^GG
SspBI 2024 TGTACA T^GTACA
StuI 1592 AGGCCT AGG^CCT
Tru9I 1037 TTAA T^TAA
Tth111I 2416 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 652 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 421 GACGTC GACGT^C
1225 GACGTC
AcsI 1033 AAATTT [GA]^AATT[CT]
2172 AAATTC
Alw44I 2344 GTGCAC G^TGCAC
2521 GTGCAC
ApaLI 2344 GTGCAC G^TGCAC
2521 GTGCAC
ApoI 1033 AAATTT [GA]^AATT[CT]
2172 AAATTC
BamHI 1538 GGATCC G^GATCC
2901 GGATCC
BbeI 121 GGCGCC GGCGC^C
1462 GGCGCC
BbrPI 490 CACGTG CAC^GTG
1017 CACGTG
BsmI 1105 GAATGC GAATGC(1/-1)
3502 GAATGC
BssSI 804 CTCGTG CACGAG(-5/-1)
856 CTCGTG
BstAPI 1235 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1418 GCAAGAATTGC
EcoT14I 1333 CCTTGG C^C[AT][AT]GG
2897 CCTTGG
HaeII 121 GGCGCC [GA]GCGC^[CT]
1462 GGCGCC
KasI 121 GGCGCC G^GCGCC
1462 GGCGCC
KspI 135 CCGCGG CCGC^GG
359 CCGCGG
NarI 121 GGCGCC GG^CGCC
1462 GGCGCC
PmaCI 490 CACGTG CAC^GTG
1017 CACGTG
PmlI 490 CACGTG CAC^GTG
1017 CACGTG
SacI 835 GAGCTC GAGCT^C
916 GAGCTC
SacII 135 CCGCGG CCGC^GG
359 CCGCGG
SfoI 121 GGCGCC GGC^GCC
1462 GGCGCC
SmaI 1553 CCCGGG CCC^GGG
1677 CCCGGG
StyI 1333 CCTTGG C^C[AT][AT]GG
2897 CCTTGG
XmaCI 1553 CCCGGG C^CCGGG
1677 CCCGGG
XmaI 1553 CCCGGG C^CCGGG
1677 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoO65I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII