Restriction map for
pF1KB4592
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BlnI BsaAI BsaHI
BsaWI BseAI BseRI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspMI
BsrBI BsrGI BssHII BstBI BstEII
BstPI BstXI Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 1053 ACATGT A^C[GA][CT]GT
AlwI 1510 GATCC GGATC(4/5)
AlwNI 935 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1466 GGGCCC GGGCC^C
AseI 1549 ATTAAT AT^TAAT
AsnI 1549 ATTAAT AT^TAAT
AvaI 19 CTCGGG C^[CT]CG[GA]G
BciVI 1036 GTATCC GTATCC(6/5)
BcnI 930 CCGGG CC^[GC]GG
BglII 377 AGATCT A^GATCT
BpmI 844 CTCCAG CTGGAG(16/14)
BsgI 109 GTGCAG GTGCAG(16/14)
BsiEI 71 CGATCG CG[GA][CT]^CG
BsoBI 19 CTCGGG C^[CT]CG[GA]G
BspHI 1433 TCATGA T^CATGA
BspLU11I 1053 ACATGT A^CATGT
BsrDI 98 GCAATG GCAATG(2/0)
BsrFI 25 ACCGGC [GA]^CCGG[CT]
BssSI 1387 CTCGTG CACGAG(-5/-1)
Bst1107I 1399 GTATAC GTA^TAC
BstYI 377 AGATCT [GA]^GATC[CT]
BstZ17I 1399 GTATAC GTA^TAC
Cfr10I 25 ACCGGC [GA]^CCGG[CT]
DrdI 1750 GACATGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 854 CCATGG C^C[GA][CT]GG
EarI 334 GAAGAG CTCTTC(1/4)
EcoT14I 854 CCATGG C^C[AT][AT]GG
Ksp632I 334 GAAGAG CTCTTC(1/4)
MaeIII 350 GTGAC ^GT[ACGT]AC
MfeI 146 CAATTG C^AATTG
MflI 377 AGATCT [GA]^GATC[CT]
MunI 146 CAATTG C^AATTG
NciI 930 CCGGG CC^[GC]GG
NcoI 854 CCATGG C^CATGG
PshBI 1549 ATTAAT AT^TAAT
PvuI 71 CGATCG CGAT^CG
RcaI 1433 TCATGA T^CATGA
ScaI 1722 AGTACT AGT^ACT
SfcI 1726 CTACAG C^T[GA][CT]AG
SmlI 754 CTTGAG C^T[CT][GA]AG
StuI 1565 AGGCCT AGG^CCT
StyI 854 CCATGG C^C[AT][AT]GG
Tsp45I 350 GTGAC ^GT[GC]AC
XbaI 1686 TCTAGA T^CTAGA
XcmI 1256 CCATAGTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 377 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1092 GTCTAC GT^[AC][GT]AC
1399 GTATAC
AccII 75 CGCG CG^CG
808 CGCG
Asp700I 646 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1123 GAACATCTTC
BalI 1298 TGGCCA TGG^CCA
1655 TGGCCA
BbsI 103 GAAGAC GAAGAC(2/6)
1747 GAAGAC
BlpI 244 GCTGAGC GC^T[ACGT]AGC
437 GCTTAGC
Bpu1102I 244 GCTGAGC GC^T[ACGT]AGC
437 GCTTAGC
BpuAI 103 GAAGAC GAAGAC(2/6)
1747 GAAGAC
BsaBI 1042 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1218 GATTGGAATC
BsaI 991 GAGACC GGTCTC(1/5)
1625 GAGACC
BsmFI 22 GGGAC GGGAC(10/14)
366 GTCCC
BstUI 75 CGCG CG^CG
808 CGCG
CelII 244 GCTGAGC GC^T[ACGT]AGC
437 GCTTAGC
EaeI 1298 TGGCCA [CT]^GGCC[GA]
1655 TGGCCA
EcoRI 1345 GAATTC G^AATTC
1365 GAATTC
HapII 26 CCGG C^CGG
930 CCGG
HindIII 410 AAGCTT A^AGCTT
713 AAGCTT
HpaII 26 CCGG C^CGG
930 CCGG
MamI 1042 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1218 GATTGGAATC
MluNI 1298 TGGCCA TGG^CCA
1655 TGGCCA
MscI 1298 TGGCCA TGG^CCA
1655 TGGCCA
MspI 26 CCGG C^CGG
930 CCGG
MvnI 75 CGCG CG^CG
808 CGCG
NspI 475 GCATGT [GA]CATG^[CT]
1053 ACATGT
PleI 119 GACTC GAGTC(4/5)
364 GAGTC
SpeI 664 ACTAGT A^CTAGT
1440 ACTAGT
TaqI 86 TCGA T^CGA
780 TCGA
TthHB8I 86 TCGA T^CGA
780 TCGA
XmnI 646 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1123 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI