################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AccI AclI AflII AgeI AhdI AlwNI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsiWI Bsp1407I BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MaeI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 2750 GACGTC GACGT^C Acc65I 137 GGTACC G^GTACC AccIII 3734 TCCGGA T^CCGGA AflIII 152 ACGTGT A^C[GA][CT]GT Alw44I 581 GTGCAC G^TGCAC ApaLI 581 GTGCAC G^TGCAC Asp718I 137 GGTACC G^GTACC BalI 4043 TGGCCA TGG^CCA BamHI 2803 GGATCC G^GATCC BsaBI 4185 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3734 TCCGGA T^CCGGA BsiEI 3504 CGGTCG CG[GA][CT]^CG BsmI 2183 GAATGC GAATGC(1/-1) BspDI 4318 ATCGAT AT^CGAT BspEI 3734 TCCGGA T^CCGGA BstXI 4134 CCAGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 4318 ATCGAT AT^CGAT DraIII 1709 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 3807 GAAGAG CTCTTC(1/4) EcoNI 4149 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 4357 GAATTC G^AATTC KpnI 137 GGTACC GGTAC^C Ksp632I 3807 GAAGAG CTCTTC(1/4) MamI 4185 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 4043 TGGCCA TGG^CCA MroI 3734 TCCGGA T^CCGGA MscI 4043 TGGCCA TGG^CCA NspI 3966 GCATGT [GA]CATG^[CT] ScaI 3818 AGTACT AGT^ACT SfcI 4221 CTACAG C^T[GA][CT]AG SgrAI 321 CACCGGCG C[GA]^CCGG[CT]G SmaI 2845 CCCGGG CCC^GGG StuI 4082 AGGCCT AGG^CCT XmaCI 2845 CCCGGG C^CCGGG XmaI 2845 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcsI 521 GAATTT [GA]^AATT[CT] 4357 GAATTC Aor51HI 2599 AGCGCT AGC^GCT 3577 AGCGCT ApoI 521 GAATTT [GA]^AATT[CT] 4357 GAATTC BsgI 4242 CTGCAC GTGCAG(16/14) 2201 GTGCAG BsmBI 149 GAGACG CGTCTC(1/5) 3077 GAGACG BstEII 3100 GGTGACC G^GT[ACGT]ACC 3325 GGTCACC BstPI 3100 GGTGACC G^GT[ACGT]ACC 3325 GGTCACC DrdI 3461 GACTCAACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3551 GACCTCCTGGTC Eco47III 2599 AGCGCT AGC^GCT 3577 AGCGCT EcoO65I 3100 GGTGACC G^GT[ACGT]ACC 3325 GGTCACC MslI 3628 CACGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4317 CATCGATGTG Tsp509I 522 AATT ^AATT 4358 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AocI ApaI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PaeR7I PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfiI SfoI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI XhoI XhoII