Restriction map for
pF1KB4802
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BbrPI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI KspI MamI MluI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1799 GTAGAC GT^[AC][GT]AC
AcsI 620 AAATTC [GA]^AATT[CT]
AflIII 970 ACATGT A^C[GA][CT]GT
AgeI 3269 ACCGGT A^CCGGT
Alw44I 878 GTGCAC G^TGCAC
ApaLI 878 GTGCAC G^TGCAC
ApoI 620 AAATTC [GA]^AATT[CT]
AspI 2022 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 775 TGCGCA TGC^GCA
BbeI 1097 GGCGCC GGCGC^C
BfaI 1873 CTAG C^TAG
BsmI 934 GAATGC GAATGC(1/-1)
BspHI 356 TCATGA T^CATGA
BspLU11I 970 ACATGT A^CATGT
EarI 2094 GAAGAG CTCTTC(1/4)
EcoT14I 3356 CCATGG C^C[AT][AT]GG
FspI 775 TGCGCA TGC^GCA
KasI 1097 GGCGCC G^GCGCC
Ksp632I 2094 GAAGAG CTCTTC(1/4)
MaeI 1873 CTAG C^TAG
MfeI 1032 CAATTG C^AATTG
MseI 572 TTAA T^TAA
MunI 1032 CAATTG C^AATTG
NarI 1097 GGCGCC GG^CGCC
NcoI 3356 CCATGG C^CATGG
NdeI 1013 CATATG CA^TATG
PflFI 2022 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3269 ACCGGT A^CCGGT
RcaI 356 TCATGA T^CATGA
SacI 3133 GAGCTC GAGCT^C
SfoI 1097 GGCGCC GGC^GCC
SmlI 648 CTTGAG C^T[CT][GA]AG
StuI 535 AGGCCT AGG^CCT
StyI 3356 CCATGG C^C[AT][AT]GG
Tru9I 572 TTAA T^TAA
Tth111I 2022 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 925 GACGCC G[GA]^CG[CT]C
1097 GGCGCC
AocI 1971 CCTCAGG CC^T[ACGT]AGG
2307 CCTGAGG
BamHI 1868 GGATCC G^GATCC
3311 GGATCC
BbsI 3324 GAAGAC GAAGAC(2/6)
2322 GTCTTC
BpuAI 3324 GAAGAC GAAGAC(2/6)
2322 GTCTTC
BsaHI 925 GACGCC G[GA]^CG[CT]C
1097 GGCGCC
BsmBI 1574 GAGACG CGTCTC(1/5)
2020 GAGACG
Bsu36I 1971 CCTCAGG CC^T[ACGT]AGG
2307 CCTGAGG
Eco81I 1971 CCTCAGG CC^T[ACGT]AGG
2307 CCTGAGG
EcoNI 2010 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2958 CCTGCGGCAGG
HgaI 925 GACGC GACGC(5/10)
2657 GACGC
Hin1I 925 GACGCC G[GA]^CG[CT]C
1097 GGCGCC
HindIII 2061 AAGCTT A^AGCTT
2296 AAGCTT
NaeI 385 GCCGGC GCC^GGC
856 GCCGGC
NgoMIV 385 GCCGGC G^CCGGC
856 GCCGGC
SfiI 2472 GGCCGGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2742 GGCCGTGCTGGCC
SmaI 1101 CCCGGG CCC^GGG
1629 CCCGGG
SphI 45 GCATGC GCATG^C
389 GCATGC
XmaCI 1101 CCCGGG C^CCGGG
1629 CCCGGG
XmaI 1101 CCCGGG C^CCGGG
1629 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco47III EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII