Restriction map for
pF1KB5021
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstEII BstPI BstUI Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 847 GTATAC GT^[AC][GT]AC
AfaI 1282 GTAC GT^AC
AflIII 894 ACATGT A^C[GA][CT]GT
AhdI 893 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 893 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 309 GTATCC GTATCC(6/5)
BsiYI 348 CCTTCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 348 CCTTCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 854 GAGAC GTCTC(1/5)
Bsp1407I 1281 TGTACA T^GTACA
BspLU11I 894 ACATGT A^CATGT
BsrGI 1281 TGTACA T^GTACA
Bst1107I 847 GTATAC GTA^TAC
BstAPI 1432 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1418 CCAGTGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 378 AGATCC [GA]^GATC[CT]
BstZ17I 847 GTATAC GTA^TAC
DraI 180 TTTAAA TTT^AAA
DsaI 17 CCATGG C^C[GA][CT]GG
Eam1105I 893 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 84 GAAGAG CTCTTC(1/4)
EcoRI 286 GAATTC G^AATTC
EcoRV 1333 GATATC GAT^ATC
EcoT22I 1439 ATGCAT ATGCA^T
HindIII 703 AAGCTT A^AGCTT
HphI 235 GGTGA GGTGA(8/7)
Ksp632I 84 GAAGAG CTCTTC(1/4)
MaeII 630 ACGT A^CGT
MflI 378 AGATCC [GA]^GATC[CT]
MslI 328 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1266 CAGCTG C[AC]G^C[GT]G
NcoI 17 CCATGG C^CATGG
NsiI 1439 ATGCAT ATGCA^T
NspI 894 ACATGT [GA]CATG^[CT]
PleI 1152 GAGTC GAGTC(4/5)
PvuII 1266 CAGCTG CAG^CTG
RsaI 1282 GTAC GT^AC
SfaNI 1438 GATGC GCATC(5/9)
SspBI 1281 TGTACA T^GTACA
TaqI 1206 TCGA T^CGA
TthHB8I 1206 TCGA T^CGA
XhoII 378 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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AlwI 247 GATCC GGATC(4/5)
379 GATCC
AspHI 637 GTGCTC G[AT]GC[AT]^C
778 GAGCAC
BanI 1034 GGTGCC G^G[CT][GA]CC
1213 GGTGCC
BmyI 637 GTGCTC G[AGT]GC[ACT]^C
778 GAGCAC
BsiHKAI 637 GTGCTC G[AT]GC[AT]^C
778 GAGCAC
BsmFI 447 GGGAC GGGAC(10/14)
967 GGGAC
Bsp1286I 637 GTGCTC G[AGT]GC[ACT]^C
778 GAGCAC
HaeIII 204 GGCC GG^CC
1142 GGCC
MaeIII 576 GTTAC ^GT[ACGT]AC
824 GTTAC
SfcI 1022 CTGTAG C^T[GA][CT]AG
1406 CTATAG
SspI 228 AATATT AAT^ATT
871 AATATT
TspRI 116 CAGTG CA[GC]TG[ACGT][ACGT]^
1419 CAGTG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
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AcsI AluI ApoI Asp700I AvaII
BbsI BbvI BfaI BpuAI BsaJI
BsmI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HinfI ItaI MaeI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI StyI TfiI Tru9I
TseI Tsp509I XmnI