Restriction map for
pF1KB5063
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AvrII
BalI BbeI BbvCI BcgI BglI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BstBI ClaI
CpoI DraI EagI EclXI Eco52I
EcoNI EcoRV EcoT22I FseI HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SspBI SspI SwaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1423 GACGTC GACGT^C
AccI 1263 GTATAC GT^[AC][GT]AC
AclI 419 AACGTT AA^CGTT
AcsI 1375 GAATTC [GA]^AATT[CT]
AflII 685 CTTAAG C^TTAAG
AflIII 2160 ACACGT A^C[GA][CT]GT
AhdI 13 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 262 AGCGCT AGC^GCT
ApoI 1375 GAATTC [GA]^AATT[CT]
Asp700I 558 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 13 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 648 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1169 TGCGCA TGC^GCA
BbrPI 2161 CACGTG CAC^GTG
BciVI 192 GGATAC GTATCC(6/5)
BclI 491 TGATCA T^GATCA
BfrI 685 CTTAAG C^TTAAG
BsaWI 599 ACCGGA [AT]^CCGG[AT]
BsrBI 322 GAGCGG CCGCTC(-3/-3)
BsrFI 398 ACCGGC [GA]^CCGG[CT]
BssHII 89 GCGCGC G^CGCGC
BssSI 1697 CACGAG CACGAG(-5/-1)
Bst1107I 1263 GTATAC GTA^TAC
BstAPI 1510 GCAACTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1986 GGTGACC G^GT[ACGT]ACC
BstPI 1986 GGTGACC G^GT[ACGT]ACC
BstZ17I 1263 GTATAC GTA^TAC
Cfr10I 398 ACCGGC [GA]^CCGG[CT]
DrdI 508 GACATGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 13 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 262 AGCGCT AGC^GCT
EcoO65I 1986 GGTGACC G^GT[ACGT]ACC
EcoRI 1375 GAATTC G^AATTC
FbaI 491 TGATCA T^GATCA
FspI 1169 TGCGCA TGC^GCA
HindIII 2275 AAGCTT A^AGCTT
MspA1I 1787 CAGCTG C[AC]G^C[GT]G
NdeI 998 CATATG CA^TATG
PflFI 648 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2161 CACGTG CAC^GTG
PmlI 2161 CACGTG CAC^GTG
PpuMI 515 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 419 AACGTT AA^CGTT
PvuII 1787 CAGCTG CAG^CTG
SmaI 1610 CCCGGG CCC^GGG
SnaBI 791 TACGTA TAC^GTA
StuI 2024 AGGCCT AGG^CCT
Tth111I 648 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1610 CCCGGG C^CCGGG
XmaI 1610 CCCGGG C^CCGGG
XmnI 558 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 200 GGCGTC G[GA]^CG[CT]C
1423 GACGTC
AfaI 790 GTAC GT^AC
794 GTAC
AocI 1585 CCTGAGG CC^T[ACGT]AGG
1911 CCTGAGG
ApaI 531 GGGCCC GGGCC^C
1956 GGGCCC
AvaI 458 CTCGGG C^[CT]CG[GA]G
1610 CCCGGG
BamHI 158 GGATCC G^GATCC
2253 GGATCC
BbsI 2308 GAAGAC GAAGAC(2/6)
1834 GTCTTC
BlpI 118 GCTCAGC GC^T[ACGT]AGC
258 GCTAAGC
Bpu1102I 118 GCTCAGC GC^T[ACGT]AGC
258 GCTAAGC
BpuAI 2308 GAAGAC GAAGAC(2/6)
1834 GTCTTC
BsaAI 791 TACGTA [CT]AC^GT[GA]
2161 CACGTG
BsaHI 200 GGCGTC G[GA]^CG[CT]C
1423 GACGTC
BsaI 1127 GAGACC GGTCTC(1/5)
1795 GAGACC
BsmBI 202 CGTCTC CGTCTC(1/5)
64 GAGACG
BsoBI 458 CTCGGG C^[CT]CG[GA]G
1610 CCCGGG
BsrDI 1165 GCAATG GCAATG(2/0)
1412 GCAATG
BstXI 693 CCACAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1754 CCAAGTCCTTGG
Bsu36I 1585 CCTGAGG CC^T[ACGT]AGG
1911 CCTGAGG
CelII 118 GCTCAGC GC^T[ACGT]AGC
258 GCTAAGC
DraIII 2035 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2161 CACGTGGTG
EaeI 947 CGGCCA [CT]^GGCC[GA]
1452 TGGCCG
EarI 25 CTCTTC CTCTTC(1/4)
1392 CTCTTC
Eco81I 1585 CCTGAGG CC^T[ACGT]AGG
1911 CCTGAGG
HaeII 262 AGCGCT [GA]GCGC^[CT]
1883 AGCGCC
Hin1I 200 GGCGTC G[GA]^CG[CT]C
1423 GACGTC
Ksp632I 25 CTCTTC CTCTTC(1/4)
1392 CTCTTC
MslI 1704 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1767 CATCCAGGTG
NcoI 1136 CCATGG C^CATGG
1703 CCATGG
PstI 1939 CTGCAG CTGCA^G
1992 CTGCAG
RsaI 790 GTAC GT^AC
794 GTAC
SacI 1042 GAGCTC GAGCT^C
1090 GAGCTC
SapI 24 GCTCTTC GCTCTTC(1/4)
1391 GCTCTTC
TfiI 750 GAATC G^A[AT]TC
821 GAATC
Tsp509I 853 AATT ^AATT
1376 AATT
XbaI 9 TCTAGA T^CTAGA
1656 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII